Preparation of sliver and selenium n ps and its characterization by dls and sem

© 2018 Journal of Microscopy and Ultrastructure | Published by Wolters Kluwer - Medknow182
Abstract
Original Article
Introduction
Nanosized materials possess desirable novel and superior
properties to macrosized materials, and homogeneous
nanoparticle suspensions play an important role in many
scientific and industrial applications, for example, in
colloidal science,[1]
nanotoxicological studies,[2]
high thermal
conductivity fluids,[3]
and nanofluidics.[4]
In these applications,
the recent experience has identified the need for a well‑defined
and accurate method of characterizing nanoparticles in
suspension. Among the available experimental methods (e.g.,
photocentrifuges, X‑ray disc centrifuges, scanning electron
microscopy (SEM), and scanning mobility particle sizer),
dynamic light scattering can provide information about
particles with sizes in the range from a few nanometers to
several micrometers. Even though the application of DLS to
particle sizing in dilute solutions has become increasingly
common because the diffusion of particles in a solution is
influenced by the kinetic and hydrodynamic conditions,
the repeatability and the uncertainty of DLS measurements
are often insufficient to accurately evaluate the real size of
nanoparticles.
Silver and its compounds are known to have antimicrobial
properties. Early in the 19th
century, 0.5% AgNO3
was used
for the treatment and prevention of microbial infections such
as ophthalmia neonatorum (by German obstetrician Carl
Aim: In our study, two different methods were used to determine the size and size distribution of the sliver and selenium nanoparticles via
dynamic light scattering (DLS) and scanning electron microscopy (SEM). Background: Nanotechnology dealing with metal and metalloid
nanoparticles has been usually applied in nearly each field of science, engineering, and technology including biology and medicine etc due
to presence of size and shape dependent unusual physical and chemical properties. In the most recent decade, numerous groups including
appreciably developed metal and metalloid nanoparticles based theranostic approaches for the treatment of almost human diseases. Amongst
many nanoparticles, recently silver and selenium nanoparticles have been broadly used in the antimicrobial coatings, textiles, paints, keyboards,
engineering, food industry, electronics, cosmetics, bio-sensing, wound dressings, and even in biomedical devices. Methods: In our study,
silver nanoparticles were prepared by using the chemical reduction method. Selenium nanoparticles (SeNPs) were synthesized by the chemical
reduction of sodium selenite by glutathione (reduced form) and stabilized by bovine serum albumin (BSA). Characterization of silver and
selenium nanoparticles samples were analyzed by dynamic light scattering (DLS) and Scanning Electron Microscopy (SEM).Conclusions: Due
to characterization by DLS technique, nanoparticles size was found the range of 79.22 nm and 178 nm for Sliver and Selenium Nanoparticles
respectively. Sliver nanoparticles shown morphological average size and shape with SEM reveals spherical shape particles with the size of
80.32 nm whereas Selenium nanoparticles shown rod shape particles with the size of 74.29 nm.
Keywords: Biomedical devices, dynamic light scattering, microbial infections, nanotechnology, scanning electron microscopy, selenium
nanoparticles
Address for correspondence: Ms. Poonam Verma,
School of Biotechnology, IFTM University, Moradabad, Uttar Pradesh, India.
E‑mail: poonam.phdbiotech@gmail.com
Access this article online
Quick Response Code:
Website:
http://www.jmau.org/
DOI:
10.4103/JMAU.JMAU_3_18
This is an open access journal, and articles are distributed under the terms of the Creative
Commons Attribution‑NonCommercial‑ShareAlike 4.0 License, which allows others to
remix, tweak, and build upon the work non‑commercially, as long as appropriate credit
is given and the new creations are licensed under the identical terms.
For reprints contact: reprints@medknow.com
How to cite this article: Verma P, Maheshwari SK. Preparation of sliver and
selenium nanoparticles and its characterization by dynamic light scattering
and scanning electron microscopy. J Microsc Ultrastruct 2018;6:182-7.
Preparation of Sliver and Selenium Nanoparticles and Its
Characterization by Dynamic Light Scattering and Scanning
Electron Microscopy
Poonam Verma, Sanjiv Kumar Maheshwari1
Department of Biotechnology, IFTM University, Moradabad, 1
Department of Bio Science and Technology, Shri Ramswaroop Memorial University,
Lucknow-Deva Road, Lucknow, Uttar Pradesh, India
[Downloaded free from http://www.jmau.org on Saturday, October 27, 2018, IP: 10.232.74.27]

Verma and Maheshwari: Preparation and characterization of sliver and selenium nanoparticles
Journal of Microscopy and Ultrastructure ¦ Volume 6 ¦ Issue 4 ¦ October-December 2018 183
Crede). When the era of antibiotics began with the discovery
of penicillin, the use of silver slowly diminished;[5]
however,
currently, due to the lack of effectiveness of conventional
drugs, the use of silver for treating infections has regained
importance. However, the use of ionic silver has one major
flaw: it is easily inactivated by complexation and precipitation.
As a result, the use of silver ions has been limited.[6]
Silver
nanoparticles (AgNPs), which are zerovalent, can be a valuable
alternative to ionic silver (Sintubin et al.,).[7]
AgNPs are a
nontoxic and safe antibacterial agent[8,9]
for the human body.
In addition, nano‑Ags are also reported to possess antifungal
activity,[10]
anti‑inflammatory properties,[11]
antiviral activity,[12]
and antiangiogenic activity.[13]
Nano‑Ags can be applied safely
in therapy when the effective concentrations against various
types of organisms have been determined.
Selenium (Se) is an essential element in human and animal
body in low concentration. It is a necessary dietary constituent
of at least 25 human selenoproteins and enzymes‑containing
selenocysteine.[14]
In the environment, it exists in many
oxidation states (−2, 0, +4, and +6) and forms such as ionic
selenite (Na2
SeO3
) and selenate (Na2
SeO4
), solid‑state
Se (0), and selenomethionine (SeMet)/selenocysteine.[15,16]
Selenium nanoparticles (SeNPs) can be synthesized through
physical methods such as laser ablation, UV radiation, and
hydrothermal techniques.[17,18]
Chemical synthesis is mediated
by precipitation, acid decomposition, and catalytic reduction
using ascorbic acid, glucose, sulfur dioxide, and sodium
dodecyl sulfate.[19,20]
Metal and metalloid nanoparticles have been frequently useful
in almost every field of science, and technology including
biomedical, due to the presence of small size‑dependent
and shape‑dependent curious physical and chemical
properties. In last decades, applications of Ag‑based and
Se‑based nanoparticles were discussed in the field of
infectious diseases treatment such as biofilms on medical
devices[21,22]
and as an antimicrobial.[8,9]
In other aspect,
other researchers Haris and Khan[23]
studied that novel
SeNPs‑enhanced photodynamic therapy of toluidine blue
O against biofilm forming Streptococcus mutans bacterial
isolates. Haris and Ahmad[24]
were studied the impact of
metal oxide (ZnO and TiO2
) nanoparticles on beneficial
soil microorganisms and their secondary metabolites. The
antibacterial potential of nanoparticles was determined by
growth kinetics of Pseudomonas aeruginosa, Pseudomonas
fluorescens, and Bacillus amyloliquefaciens. Soni[25]
was
discussed the biodegradable nanoparticles for delivering
drugs and silencing multiple genes or gene activation in
diabetic nephropathy. Chauhan et al.[26]
were also discussed the
applications of nanotechnology in forensic investigation that
reveals the hidden evidence, which can prove to be helpful for
the forensic scientists to give an outcome to their investigation.
Materials and Methods
Chemicals
All chemicals of silver and selenium nanoparticles used in
this experiment were highest purified and purchased from
Central Drug House (P) Ltd, Delhi, India. Distilled water
was also used for the preparation of reagents. Further study
was done at Department of Biotechnology, IFTM University
Moradabad, India.
Preparation of nanoparticles using the chemical reduction
method
Preparation of silver nanoparticles
In this study, silver colloid was prepared by using the chemical
reduction method. All solutions of reacting materials were
prepared in distilled water.
• Solution 1: 0.0849 g of silver nitrate (AgNO3
) was
dissolved in 500 ml of distilled water, and then the solution
was heated to boiling
• Solution 2: Then, 1 g of trisodium citrate (C6
H5
O7
Na3
)
was dissolved in 100 ml of distilled water
• Working solution: Five ml of trisodium citrate was
added to 500 ml of AgNO3
after boiling (drop by drop).
During the process, the solution was forcefully mixed.
The solution was left on hot plate for 2 h at 90°C for
heated (uncontinuous manner) then it was cooled at room
temperature, the color was reddish‑green[27]
appeared.
PreparedAgNPs were collected in 1.5 ml Eppendorf tube
and kept at room temperature for further experiment.
Preparation of selenium nanoparticles
SeNPs were synthesized by the chemical reduction of sodium
selenite by glutathione (reduced form) and stabilized by bovine
serum albumin (BSA). Specifically, 3 ml of 25 mM Na2
SeO3
,
3 mL of 100 mM GSH, and 0.15 g BSA were added to 9 ml of
double‑distilled water (dH2
O) in a sterile cabinet.All solutions
were made in a sterile environment using a sterile cabinet and
double‑distilled water. After mixing the reactant solution,
1M NaOH was added to bring the pH of the solution to the
alkaline media. SeNPs were formed immediately following
the addition of NaOH as visualized by a color change of the
reactant solution from clear white to clear red color. Then,
SeNPs were collected by centrifuging (Cooling centrifuge
C‑24 BL) the solution at 13,000 rpm at −4°C temperature with
30‑min duration sterilized by ultraviolet light exposure, before
use in bacteria experiment.[28]
Prepared SeNPs were collected
in 1.5 ml Eppendorf tube and were kept at room temperature
for further experiment.
Characterization of silver and selenium nanoparticles
Particle size analyzer (dynamic light scattering)
DLS was used to measure the hydrodynamic diameter. The
particlesizeoftheAgNPssamplewasanalyzedbyDLSusingthe
Litesizer™ 500 under the following conditions (at wavelength
658 nm,particleabsorptioncoefficient0.01,diffusioncoefficient
6.2 µm²/s, water refractive index 1.33, and intensity 79.67 nm).
To measure the particle size, a volume of 0.1 ml of the AgNPs
solution in 2 ml of deionized water (DI H2
O) was placed in a
polystyrene cuvette and measured at 25°C.[29]
SeNP sample was analyzed by DLS using the Microtrac,
Bluewave model, Germany, under the following conditions

Journal of Microscopy and Ultrastructure ¦ Volume 6 ¦ Issue 4 ¦ October-December 2018184
(at water refractive index 1.33 and wavelength 780 nm). To
measure the particle size, a volume of 0.1 mL of the SeNPs
solution in 2 mL of deionized water (DI H2
O) was placed in
a polystyrene cuvette and calculated at 25°C temperature.[29]
Scanning electron microscopy
SEM is a type of electron microscope that takes the image to
the sample by scanning with a high‑energy beam of electrons in
a raster scan pattern. The electrons interact with atoms that can
make up the sample producing signals that hold information
about the sample’s surface topography, composition, and other
properties such as electrical conductivity. Nanoparticles of the
silver colloid were prepared using chemical reduction method
whereas SeNPs were synthesized by the reduction of sodium
selenite by glutathione (reduced form) and stabilized by BSA.
Nanoparticles (Ag and Se) were sterilized by ultraviolet light
in laminar air flow. The sterilized nanoparticles were carefully
mounted on SEM stubs by using adhesive tape and uniformly
coated with Gold. Both nanoparticles samples were placed
in a sample chamber of SEM (JEOL JSM‑6490 LV, Japan)
and scanning was performed under different magnifications
ranging from ×15,000 to ×35,000 and voltage 20–30 kV.[27‑30]
Results
Preparation of silver and selenium nanoparticles
AgNPs synthesis adopting chemical reduction process was
primarily confirmed by the color change in the reaction
mixture from pale yellow to reddish‑green clearly indicating
to the synthesis of AgNPs.[27]
The reddish‑green color’s
formation was observed within 10 min after the dropwise
addition of trisodium citrate to the silver nitrate solution. The
antibacterial activity of AgNPs (consisting of silver nitrate)
and nanoparticle‑coated face masks to protect against biofilm
forming infectious agent Staphylococcus aureus. AgNPs are
now extensively used as antimicrobial agents and utilized in
the development of antimicrobial dressings and anti‑microbial
medical devices.
SeNPs were synthesized by the reduction of sodium selenite by
glutathione (reduced form) and formed immediately following
the addition of NaOH (make alkaline medium) as visualized
by a color change of the reactant solution from clear white
to clear red.[28]
SeNPs were then collected by centrifuging
(Cooling centrifuge C‑24) at 13,000 rpm at −4°C for 30 min
sterilized by ultraviolet light exposure.
Particle size analyzer (dynamic light scattering)
Particle size was determined using DLS measurement. The
hydrodynamic diameters of AgNPs and SeNPs in aqueous
solution were determined using DLS.
The particle size of theAgNPs sample was analyzed using DLS
using the Litesizer™ 500 found 79.22 nm [Table 1] size under
the following conditions: diffusion coefficient 6.2 μm²/s, water
refractive index 1.33, intensity 79.67 nm, and temperature
25°C[31]
under the 658‑nm laser wavelength with an angle
detection of 175° (backscatter). DLS method has several
pitfalls, in particular, with respect to the influence of dust
particles or small amounts of large aggregates in addition to a
main component of distinctly smaller size.[32]
In many chemical
companies, sedimentation velocity analysis in the analytical
ultracentrifuge has been a standard technique for studying size
of the colloidal particles, frequently using noncommercial
apparatus.[33]
Figure 1 shows the size distribution by the
intensity of AgNPs in solution and correlation function of
AgNPs shows in Figure 2.
SeNP size sample was analyzed using DLS using the
Microtrac, Bluewave model, Germany, in the range of 178 nm
[Table 1], under the following conditions: laser wavelength 780
nm, water refractive index 1.33 at room temperature (25°C).[31]
Figure 3 shows the size distribution by particles number of
SeNPs in solution and size distribution by volume particles
of SeNPs shows in Figure 4.
Scanning electron microscopy
The shape and size of the silver and SeNPs were measured
using SEM. Particle morphology shape and size with SEM
reveal that spherical shape with the size of the particle was
in the range 80.32 nm for AgNPs [Figure 5, Table 1] whereas
rods shape particles with the size of the particle were in the
range 74.29 nm [Table 1] for SeNPs [Figure 6]. Smaller‑sized
Ag and Se nanoparticles have many positive attributes, such
as good conductivity, chemical stability, and catalytic and
antibacterial activities, which would make them suitable for
many practical applications.
A scanning electron microscope (SEM) is a type of electron
microscope that produces the sample images due to scanning
the surface of the object with the focused beam of electrons
and widely considered the gold standard for nanoparticle
characterization.
Discussion
Silver nanoparticles and selenium nanoparticles
characterization by particle size analyzer (dynamic light
scattering)
Two main types of particle size analyzers, i.e., DLS and laser
Figure 1: Dynamic light‑scattering image of silver nanoparticles size
distribution by the intensity in solution

particle analyzers. DLS, one of the most popular light‑scattering
modalities can probe the size distribution of small particles at
the scale from submicron down to one nanometer in solution
or suspension using a monochromatic light source.[34]
DLS was
used to measure the hydrodynamic diameter. DLS analyzers
measure the particles in solutions in the 0.6 nm–6000 nm range
of any nanoparticle. The main advantage of DLS is the short
time required to perform the measurement and the relatively
low cost of the apparatus, therefore, DLS become the preferred
method for nanoparticle sizing.[32]
The DLS technique measures the diffusion coefficient of
suspended nanoparticles undergoing Brownian motion in a
solution by analyzing the fluctuating scattered intensity from
the nanoparticles in the solution. The scattered light from
the suspension of nanoparticles fluctuates in a characteristic
time scale, i.e., inversely proportional to the particle diffusion
coefficient. This can provide information about particles
with sizes in the range from a few nanometers to several
micrometers. Even though the application of DLS to particle
sizing in dilute solutions has become increasingly common
because the diffusion of particles in a solution is influenced by
the kinetic and hydrodynamic conditions, the repeatability and
the uncertainty of DLS measurements are often insufficient to
accurately evaluate the real size of nanoparticles. In the case of
a concentrated solution, these effects become more significant,
and therefore, a process, extrapolating the concentration and
scattering angle to extremely low values, is used to obtain the
real size of nanoparticles in solutions.[35,36]
Figures 1and3showtheDLSpicturesofAgNPssizedistribution
by the intensity in solution and SeNPsize distribution by particle
number, respectively, as well as Figures 2 and 4 show the DLS
pictures ofAgNPs of the correlation function and DLS image of
SeNPs size distribution by particles volume, respectively. The
particle size determined by DLS detector was slightly larger
than the nominal size, probably because both methods measure
a hydrodynamic size, rather than physical size.
In our result, we noticed that the synthesized AgNPs were
polydispersed and polydispersity index was found in 18.9%,
and AgNPs size 79.22 nm was analyzed. With respect to
the observations made in the present study most literature
report a similar particle size ranging of AgNPs from 55 to
85 nm by DLS[37]
whereas for SeNP size found 178 nm was
analyzed. Certain reports have, however, documented a very
less particle size range 3–18 nm[38]
and closely similar size
range (30–150 nm) was found by Sarkar et al.[31]
Silver nanoparticles and selenium nanoparticles
characterization by scanning electron microscopy
Nowadays,numerousmicroscopictechniquesarecommercially
available, whenever transmission electron microscopy and
SEM are the most popular microscopes for the analysis of the
nanoparticles.
The shape and size of the silver and SeNPs were measured
using SEM. The particle morphology shape and size with
SEM reveal that spherical shape with the size of the particles
Figure 2: Dynamic light‑scattering image of silver nanoparticles of
correlation function Figure 3: Dynamic light‑scattering image of selenium nanoparticles size
distribution by number of particles
Figure 4: Dynamic light‑scattering image of selenium nanoparticles size
distribution by volume of particles
Figure 5: Scanning electron microscopy image of silver nanoparticles

Journal of Microscopy and Ultrastructure ¦ Volume 6 ¦ Issue 4 ¦ October-December 2018186
was in the range 80.32 nm forAgNPs [Figure 5] showed close
similarity with our result ranging from 60 to 80 nm[39‑42]
whereas
rods‑shape particles with the size of the particles was in the
range 74.29 nm for SeNPs [Figure 6], showed similarity with
our result ranging from 50 to 150 nm.[43]
Conclusion
Silver and SeNPs were successfully prepared by using the
chemical reduction method. Both nanoparticles preparation
confirmation was done by qualitative analysis, i.e., AgNPs
appeared as a reddish‑green color and SeNPs appeared as
a clear white to clear red color, and characterization of NPs
was shown by particle size analyzer (DLS) as well as SEM.
By DLS, nanoparticles size was found the range of 79.22 nm
and 178 nm for AgNPs and SeNPs, respectively, and through
SEM, nanoparticles size was found the range of 80.32 nm
with spherical as well as 74.29 nm with rods shape of AgNPs
and SeNPs, respectively. These nanoparticles can be used as
a therapeutic drug for various diseases such as antibacterial,
antifungal, antiviral, biofilm eradication, and cancer treatment.
Smaller sizes of nanoparticles have great capacity to eradicate
any disease due to the high surface area.
Financial support and sponsorship
Nil.
Conflicts of interest
There are no conflicts of interest.
References
1. Brambilla G, Masri D E, Pierno M, Berthier L, Cipelletti L, Petekidis G,
SchofieldAB.Probingtheequilibriumdynamicsofcolloidalhardspheres
above the mode-coupling glass transition Phys. Rev. Lett 2009;102:1-4.
2. Iker MB, Dorota W, Patrick H, Jonathan S, Iseult L, Kenneth D.
Characterisation of nanoparticle size and state prior to nanotoxicological
studies J. Nanopart. Res 2010;12:47–53.
3. Madrid AP, Lapas LC, Rubi JM. Heat exchange between two interacting
nanoparticles beyond the fluctuation-dissipation regime Phys. Rev. Lett
2009;103:1-4.
4. Schoch RB, Han J, Renaud P. Transport phenomena in nanofluidics Rev.
Mod. Phys 2008;80:839-883.
5. Klasen HJ. Historical review of the use of silver in the treatment of
burns. I. Early uses. Burns 2000;26:117-130.
6. Atiyeh BS, Costagliola M, Hayek SN, Dibo SA. Effect of silver on burn
wound infection control and healing: review of the literature. Burns
2007;33:139-148.
7. Sintubin L, De Windt W, Dick J, Mast J, Van Der Ha D, Verstraete W,
et al. Lactic acid bacteria as reducing and capping agent for the fast and
efficient production of silver nanoparticles. Appl Microbiol Biotechnol
2009;84:741-9.
8. Verma P. A Review on synthesis and their antibacterial activity of Silver
and Selenium nanoparticles against biofilm forming Staphylococcus
aureus. World Journal of Pharmacy and Pharmaceutical Sciences
2015;4:652-77.
9. Verma P, Maheshwari SK. Minimum Biofilm Eradication Concentration
(MBEC) assay of Silver and Selenium Nanoparticles against Biofilm
forming Staphylococcus aureus. JMSCR 2017;5:20213-22.
10. Kim KJ, SungWS, Suh BK, Moon SK, Choi JS, Kim JG, et al.Antifungal
activity and mode of action of silver nano-particles on Candida albicans.
Biometals 2009;22:235-42.
11. Nadworny PL, Wang J, Tredget EE, Burrell RE. Antiinflammatory
activity of nanocrystalline silver in a porcine contact dermatitis model.
Nanomedicine 2008;4:241-51.
12. Rogers JV, Parkinson CV, Choi YW, Speshock JL, Hussain SM. A
preliminary assessment of silver nanoparticle inhibition of monkeypox
virus plaque formation. Nanoscale Res Lett 2008;3:129-33.
13. Gurunathan S, Lee KJ, Kalishwaralal K, Sheikpranbabu S,
Vaidyanathan R, Eom SH. Antiangiogenic properties of silver
nanoparticles. Biomaterials 2009;30:6341-50.
14. Zhang J, Spallholz J. Toxicity of selenium compounds and nano-
selenium particles. In: Casciano D, Sahu SC, editors. Handbook of
Systems Toxicology. West Sussex, UK: John Wiley and Sons 2011.
15. Biswas K, Barton L, Tsui W, Shuman K, Gillespie J, Eze C. A novel
method for the measurement of elemental selenium produced by
bacterial reduction of selenite. J Microbiol Methods 2011;86:140-4.
16. Bo Li D, Cheng Y, Wu C, Li W, Li N, Yang Z, et al. Selenite reduction
by Shewanella oneidensis MR-1 is mediated by fumarate reductase in
periplasm. Sci Rep 2014;4:3735.
17. Iranifam M, Fathinia M, Sadeghi T, Hanifehpour Y, Khataee A, Joo S. A
novel selenium nanoparticles-enhanced chemiluminescence system for
determination of dinitrobutylphenol. Talanta 2013 107:263-9.
18. Overschelde O, Guisbiers G, Snyders R. Green synthesis of selenium
nanoparticles by excimer pulsed laser ablation in water. Appl Mater
2013;1:042114.
19. Zhang Y, Wang J, Zhang L. Creation of highly stable selenium
nanoparticles capped with hyperbranched polysaccharide in water.
Langmuir 2010;26:17617-23.
20. Dwivedi C, Shah C, Singh K, Kumar M, Bajaj P.An organic acidinduced
synthesis and characterization of selenium. Nanopart J Nanotechnol
2011. p. 1-6.
21. Verma P, Maheshwari SK, Mathur A. A review on Bacterial Biofilm
Formation and Disassembly. Int. J Pharm Sci Res 2013;4:2900-6.
22. VermaP,SinghS.ClinicaldistributionofBiofilmformingStaphylococcus
aureus and its sensitivity against some antibiotics. World Journal of
Pharmacy and Pharmaceutical Sciences 2015;4:942-52.
23. Haris Z, Khan AU. Selenium Nanoparticle Enhanced Photodynamic
Therapy against Biofilm forming Streptococcus mutans. Int. J. Life. Sci.
Scienti. Res 2017;3:1287-94.
24. Haris Z, Ahmad I. Impact of Metal Oxide Nanoparticles on Beneficial
Soil Microorganisms and their Secondary Metabolites. Int. J. Life. Sci.
Scienti. Res 2017;3:1020-30.
Figure 6: Scanning electron microscopy image of selenium nanoparticles
Table 1: Measurement of the nanoparticles (Ag and Se)
shape and size under different microscope
Characteristics DLS SEM
AgNPs SeNPs AgNPs SeNPs
Size (nm) 79.22 178 80.32 74.29
Shape ‑ ‑ Spherical Rod
DLS: Dynamic light scattering, SEM: Scanning electron microscope,
AgNPs: Silver nanoparticles, SeNPs: Selenium nanoparticles

25. Soni NO. Biodegradable Nanoparticles for Delivering Drugs and
Silencing Multiple Genes or Gene activation in Diabetic Nephropathy.
Int. J. Life. Sci. Scienti. Res 2017;3:1329-38.
26. Chauhan V, Singh V, Tiwari A. Applications of Nanotechnology in
Forensic Investigation. Int. J. Life. Sci. Scienti. Res 2017;3:1047-51.
27. Sileikaite A, Puiso J, Prosycevas I, Tamulevicius S. Investigation of
Silver Nanoparticles Formation Kinetics during Reduction of Silver
Nitrate with Sodium Citrate. Materials Science 2009;15:21-7.
28. Tran PA, Webster TJ. Selenium nanoparticles inhibit Staphylococcus
aureus growth. Int J Nanomedicine 2011;6:1553-8.
29. BootzA,VogelV,SchubertD,KreuterJ.Comparisonofscanningelectron
microscopy, dynamic light scattering and analytical ultracentrifugation
for the sizing of poly (butyl cyanoacrylate) nanoparticles. European
Journal of Pharmaceutics and Biopharmaceutics 2004;57:369-75.
30. Razi MK, Maamoury RS, Banihashemi S. Preparation of nanoselenium
particles by water solution phase method from industrial dust. Int. J.
Nano. Dim 2011;1:261-7.
31. Sarkar J, Dey P, Saha S, Acharya K. Mycosynthesis of selenium
nanoparticles. Micro & Nano Letters 2011;6:599-602.
32. Berne B, Pecora R. Dynamic Light Scattering with Applications to
Chemistry, Biology, and Physics, Dover Publications, Mineola, NY
2000.
33. MachtleW. High-resolution, submicron particle size distribution analysis
using gravitational-sweep sedimentation, Biophys. J 1999;76:1080-91.
34. Sapsford KE, Tyner KM, Dair BJ, Deschamps JR, Medintz IL.
Analyzing nanomaterial bioconjugates: a review of current and
emerging purification and characterization techniques. Anal Chem.
2011; 83:4453-88.
35. Johnson CS and Gabriel DA. Laser Light Scattering (New York: Dover),
1981.
36. Takahashi K, Kato H, Saito T, Matsuyama S, Kinugasa S. Precise
measurement of the size of nanoparticles by dynamic light scattering
with uncertainty analysis Part. Part. Syst. Charact 2008;25:31-8.
37. Mukherjee S, Chowdhury D, Kotcherlakota R, Patra S, Vinothkumar B,
Bhadra MP, Sreedhar B, Patra CR. Potential Theranostics Application
of Bio-Synthesized Silver Nanoparticles (4-in-1 System. Theranostics
2014;4:316-35.
38. Sharma G, Sharma AR, Bhavesh R, Park J, ET, et al. Biomolecule-
Mediated Synthesis of Selenium Nanoparticles using Dried Vitis
vinifera (Raisin) Extract. Molecules 2014;19:2761-70.
39. Namasivayam SKR, Preethi M, and Arvind Bharani RS. Biofilm
Inhibitory effect of Silver nanoparticles coated Catheter against
Staphylococcus aureus and Evaluation of its Synergistic effect with
Antibiotics. International Journal of Biological & Pharmaceutical
Research 2012;3:259-65.
40. El-Kheshen AA, El-Rab SFG. Effect of reducing and protecting agents
on size of silver nanoparticles and their anti-bacterial activity. Der
Pharma Chemica 2012;4:53-65.
41. DimitrijevicR,CvetkovicO,MiodragovicZ,SimicM,ManojlovicD,and
Jovic V. SEM/EDX and XRD characterization of silver nanocrystalline
Thin film prepared from organometallic solution precursor. J. Min.
Metall. Sect. B-Metall 2013;49B:91-5.
42. Devaraj P, Kumari P, Aarti C, Renganathan A. Synthesis and
Characterization of Silver Nanoparticles Using Cannonball Leaves
and Their Cytotoxic Activity against MCF-7 Cell Line. Journal of
Nanotechnology 2013;2013:1-5.
43. RamamurthyCH,SampathKS,ArunkumarP,KumarMS,SujathaV,Prem
Kumar K, Thirunavukkarasu C. Green synthesis and characterization of
selenium nanoparticles and its augmented cytotoxicity with doxorubicin
on cancer cells. Bioprocess Biosyst Eng 2013;36:1131-9.

Preparation of sliver and selenium n ps and its characterization by dls and sem

More Related Content

What's hot (20)

Similar to Preparation of sliver and selenium n ps and its characterization by dls and sem (20)

More from SSR Institute of International Journal of Life Sciences (20)

Recently uploaded (20)

Preparation of sliver and selenium n ps and its characterization by dls and sem