Presentation jayshree
- 1. M.V.P.Samaj’s K.T.H.M. COLLEGE, GANGAPUR ROAD, NASHIK – 422 002 DEPARTMENT OF ZOOLOGY WELCOMES
- 2. TOXICITY EVALUATION OF PYRIDONE DERIVATIVES ON Sitophilus Oryzae Linn
- 3. By Mrs. Jayshree Vitthal Shinde Under the guidance of Dr. A. E. Desai
- 4. INTRODUCTION • Insect existed at least 20 million yrs ago. • Grain pests e.g. Rodents, Nematodes, Fungi • Classification of pests • Plants extracts/ botanical insecticides • Synthetic chemical compounds
- 5. Aim Of This Project • Toxicity Evaluation of Synthesized Chemical Compounds on Stored Grain Pests. • To Protect Stored Grains.
- 6. Material & Methods • Collection & Culture Of Sitophilus oryzae Linn • Synthesis of chemical compounds.
- 7. Observation Table Table no 1: 2,5dihydro-7,8dimethoxy-4(3-methoxyphenyl)-2-oxo-1H-in deno (1,2-b)pyridine-3carbonitrile. Sr.no. Dose No.of Mortality in insects for ppm released 6hrs 12hrs 18hrs 24hrs 1 5 10 1 2 3 6 2 10 10 2 3 3 7 3 15 10 2 3 4 8 4 20 10 3 3 4 9
- 8. Table no 2: 2,5dihydro-7,8dimethoxy-4(3,4-dimethoxyphenyl)-2- oxo-1H-in deno (1,2-b)pyridine-3carbonitrile. Sr.no. Dose No.of Mortality in insects for ppm released 6hrs 12hrs 18hrs 24hrs 1 5 10 2 2 2 5 2 10 10 2 3 3 6 3 15 10 3 3 3 8 4 20 10 2 3 4 9
- 9. Table no 3: 2,5dihydro-7,8dimethoxy-4(3,4,5-trimethoxyphenyl)-2-oxo-1H- in deno (1,2-b)pyridine-3carbonitrile. Sr.no. Dose No.of Mortality in insects for ppm released 6hrs 12hrs 18hrs 24hrs 1 5 10 1 2 3 6 2 10 10 2 3 3 8 3 15 10 2 3 4 9 4 20 10 3 3 3 9
- 10. Table no 4: 2,5dihydro-7,8dimethoxy-4(thiophen-2-yl)oxo-1H-in deno (1,2-b)pyridine-3carbonitrile. Sr.no. Dose No.of Mortality in insects for ppm released 6hrs 12hrs 18hrs 24hrs 1 5 10 2 2 3 6 2 10 10 2 3 3 7 3 15 10 2 3 4 9 4 20 10 3 2 3 9
- 11. Result Table No. 9 - Comparative Results Chemical Time in Mortality LC50 ppm Regression eqn Heterogencity VarianceFinancial 2 Comp Hrs. ± S.E. Y-a+b x (n-1) Limit 0 5 10 15 20 A=y-bx Comp I 24 0 60 70 80 90 1.5515 ± 0.9982 4.438 2.46 0.997 m1=0.0951 (101) m2=3.0075 Comp 2 24 0 50 60 80 90 1.3357 ± 0.7354 -2.539 3.908 0.54 m1=0.1044 (104) m2=2.567 Comp 3 24 0 60 80 90 90 1.5885 ± 0.9567 2.983 4.447 0.905 m1=0.1034 (105) m2=3.0736 Comp 4 24 0 60 70 90 90 1.5134 ± 0.8654 2.936 4.315 0.802 m1=0.0311 (106) m2=2.9957
- 12. Graph
- 13. Discussion • There is significant increase in mortality from low to high dose of chemical compound. • S. oryzae, R. dominica, T. castaneum shows high mortality when exposed to biogass in PVC bins (Bennett, 2003). • Azadirachtin exhibited good efficiency against Musca nobulo L, Culex fatigans, Tribolium castaneum (Paul et, al.1963). • PN101, LC50=1.5515+0.9982, PN104, LC50=1.3357+0.7354, PN105,LC50=1.5134+0.8954, PN106, LC50=1.5885+0.9567.
- 14. References • Bennett S. (2003). Stored Product insects, available on: www.thepiedpiper.co.uk/th7.htm. (Accessed 22march 2012) • Finney D.(1971). Probit analysis Cambridge University press, London. • Paul, C.F, R.S.Dixit and P.N. Agarwal. (1963). Evaluation of the insecticidal properties of the seed oil and leaf extract of the common Indian neem, Azadirachta indica L. Sci. Cult. 29:412-413