Proposal presentation lab on chip
- 1. Brent Gifford Jerry Jackson John Trishler Nathaniel Haller BIEN 400 Louisiana Tech University
- 2. Cancer is the second leading cause of death in the United States Leukemia and Lymphoma are two significant blood cancers Cancer Treatments are expensive, low survivability, chance of resurfacing. Early Detection is Half the Battle Need for an early detection screening device
- 3. Leukemia is cancer of the blood cells, which starts in the bone marrow of the bones. Leukemia symptoms: fatigue, paleness, weight loss, repeated infection, bruising, nosebleeds. Sometimes no symptoms Hard to detect early
- 4. Our device would fill a need that would allow health care professionals to administer a screening process that would allow for early detection of blood cancers. Our device will address the need of a preliminary test that will greatly reduce the amount of test that the clinics will need to do, it will also address the need for a screen test to detect blood cancers earlier thus increasing survival rates.
- 5. Product Description Design Specification Separate cells in the Blood The micro-fluidic device should separate the blood cells from the abnormal blast cells which will be shunted to a separate channel. Detect threshold of Blast Cells Detector will then give a positive or negative result based on a threshold value in the device. Ease of Use The device must me simple enough that a health care professional with limited training can pick it up and use it with little instruction Reliability The device should give results with an accuracy range of at least 90%
- 6. Cancer Detection Technology Pros Cons Post Method Simple to design and manufacture Cells can becomes stuck between the posts blocking the filter Electrical Method Separates the cells into different channels Electrodes must be implanted into the due to electrical differences of the device, increases difficulty of membranes on the individual cells. manufacturing. Doughnut Method Capable of separating the cells through a Too much pressure can cause the cells to series of channels with pressure. lyses Electrical Detection Capable of determining positive or negative Electrodes must be implanted into the result based on electrical threshold from device, increases difficulty of membrane potential resistance manufacturing. Chemical Detection Use of dye to stain blast cells. Dye needs to be introduced previous to sampling, finger prick might not work.
- 7. Electrical Separation with Electrical Resistance Detection Use electrical charge to separate the cells Use electrical resistance across a threshold to detect Doughnut Filter with Electrical Resistance Detection Use doughnut filter with pressure to separate cells into channels Use electrical resistance across a threshold to detect Other Combinations If previous combinations fail
- 8. Red Blood Cells 6 to 8 um White Blood Cells Neutrophils 12-15 um Eosinophils 12-15 um Lymphocytes 6-18 um Monocytes 12-20 um Basophils 12-15 um Cancer Cells Abnormal White Blood Cells (BLAST cells) Massive in size in comparison to white blood cells Figure 1: A Wright's stained bone marrow aspirate smear from a patient with precursor B-cell acute lymphoblastic leukemia
- 9. Other Lab-On-Chip devices Genetic analysis Cell Separator Overall Micro-fluidic technology is viable
- 10. Math Modeling Giesekus Model Navier-Stokes Simulation spherical cell on rectangular microchannel Figure2: Schematic of lateral migration and deformation of a deformable drop in a microchannel.
- 11. Test various technologies for integration into device. Test micro-fluidic designs for best possible separation Using the micro-fluidic design that gives the best separation will allow us to more accurately detect blast cells that are a sign of blood cancer. Test Detection devices A reliable device that detects threshold values of blast cells to give a positive or negative result for cells collected in the micro- fluidic device. Integrate feasible technologies into a single device Disposable needle and chip The chip needs to collect and separate the blood and blast cells Reusable Detector The reusable device needs to give an output weather blast concentrations pass or fall below a specific threshold
- 12. Develop prototype The prototype should be developed with the main users, health care professionals in mind. The device should be quick to use with results given in a manner of minutes The chip and needle should separate from the detector with the detector working without malfunction for and extended number uses over a period of years. Field Testing The device should be tested by those without blood cancer and those with in a clinical setting for final viability.
- 13. Goal is to detect cancerous white blood cells in whole blood samples Detecting cancerous white blood cells to increase blood cancer detection rate Use both disposable and reusable systems Disposable- needle and microchannel chip Reusable- Electric detection device Yes or No
- 14. Based off of cell properties Main channel Smaller channels Electrical method Electrical Detector
- 15. Based off Cell Properties Doughnut Design Pressure Push Electrical Detector
- 16. Microfabrication Photolithography Masks Testing Nanoparticles Blood (non-cancerous) Blood (cancerous)
- 17. Price Per Number Total Our Cost Component Component Required Price($) ($) ($) Prototype to be developed UV Oven 1 500 500 Donated for $ 2750+ Includes donated Spin Coater 1 2000 2000 Donated components SU-8 Photoresist and Etchant TBD 20 TBD Donated Overall cost of product not Silicon Wafer to exceed $10000.00 TBD 300 TBD Donated Substrate depends on number of Peristaltic Pump TBD 350 TBD TBD chips made No direct competitors to Glass TBD 20 TBD Donated our design Electrodes TBD 40 TBD TBD Several traditional tests Other Expenses 200
- 18. Two teams of two One Team of four Incorporate technologies Team 1 into one device Micro-fluidic device Designing and prototyping the disposable part. Testing for reliability Team 2 Detector Field testing with group Electrical resistance design volunteers and blood Reusable with input for cultures. the chip and needle
- 19. Final Presentation Field Testing Development of Prototype and Initial Testing Design of Prototype Design of individual components Research and Initial Testing September October November December January February March April May