Rajesh Resume2016
- 1. CV: Rajesh Kumar Gupta Rajesh Kumar Gupta Contact Number: 91-9818602532; 91-9868656031 E-Mail ID: rajesh.raj.25784@gmail.com,dr.rajeshbt@rediffmail.com Objective- To seek ample opportunities for development of self-contributing effectively to Crop improvement. Education S.No. Name of the Examination Name of the Board/ University Year of passing Grade/Division & % of marks in aggregate Subject taken 1. Ph. D NRC on Plant Biotechnology, New Delhi 2010- Pursuing Biotechnology 2. M.Sc. CCS University, Meerut 2009 71.15 Biotechnology, Biochemistry, Biostatistics, Genetics etc 3. B.Sc. CSJM University, Kanpur 2006 53.25 Chemistry, Zoology & Botany 4. Intermediate U.P. Board 2001 54.0 Hindi, English, Physics, Chemistry & Biology 5. High School U.P. Board 1999 65.33 Hindi, Eng., Math, Science, Social Science Awards ICMR-SRF-: March 2013- March 2016. GATE Qualified-: 2010 (Biotechnology). Present Status Presently I am working as Senior Research Scholar in ICAR- NRC on Plant Biotechnology, IARI, New Delhi in molecular biology lab involving in project “modification of wheat gluten proteins responsible for allergy in human” under supervision of Dr. PK Mandal, Principal Scientist (also my PhD co- supervisior). Technical Skills Molecular Biology: Genomic DNA, Plasmid DNA and RNA isolations from plant cell, Nano Drop Spectrophotometer, CDNA synthesis, PCR, Real Time PCR, Agarose Gel Electrophoresis, Gel Documentation System, Transformation, Cloning, Restriction digestion & Colony PCR. Biochemistry Techniques: Extraction of enzyme/protein, Enzyme assay, SDS-PAGE, Nitrogen estimation, UV-Spectrophotometer, Titration (Neutralization, Complexometric & Precipitation), TLC, Liquid column chromatography, Affinity column chromatography, HPLC, Gas Chromatography (GC), GC-MS and Formulation. Page 1
- 2. CV: Rajesh Kumar Gupta Immuno Techniques: ELISA test, RID (Radial-immuno diffusion), Affinity column chromatography, Ultramicrotome & Light microscope. Bioinformatics: Primer designing, Analysis of molecular data using NTSYS pc software, Knowledge of using on-server bioinformatics tools like NCBI, CLUSTAL W, PROTEIN MODELLING tools, ZDOCK and Expasy. Knowledge of using off-server bioinformatics tools like Bio edit, Gene runner and Good exposure to internet browsing through Internet explorer search engines. Biostatistics: t-test, Chi squared test, Regression & ANOVA. Publications A.K. Pathak, Rajesh Kr Gupta, Sunil Kr Singh, P. Ray. choudhury and Asit. B Mandal (2016) Assessment of DNA polymorphism and genetic diversity among heat tolerant and susceptible wheat (Triticum aestivum L.) varieties by ISSR markers. Plant Genetic Resources- Characterization and utilization, April 2016 (communicated; Impact factor 0.58) Jayanthi Madhavan, Jankiram Tolety, Rajesh Gupta and Pranab Kumar Mandal (2016). Plantlet regeneration via two different pathways and detection of culture induced variation using molecular markers in chrysanthemum cv Pusa centenary. Horticulture, Environment, and Biotechnology. Aug 2016 (communicated; Impact factor 0.458) Rajesh Kr Gupta, Madhu Gupta, P. Ray Choudhury, and Asit B. Mandal (2016) Transferability of rice microsatellite primers (SSR) across six major. Advances in Life Sciences, 5(2): 377-383 (NASS Rating 3.56) Rajesh Kumar Gupta, Vijay Yadav, Partha Roy Choudhury and Asit B. Mandal (2016). Assessment of genetic purity of rice hybrids and their parental lines using SSR markers. Advances in life sciences, 5(3): 970-975 (NASS Rating 3.56) Shashi Bhushan Chauhan, Rajesh Kumar Gupta, Partha Roy Choudhury and Asit B. Mandal (2016). Molecular characterization of cotton hybrids and their parental lines using SSR markers. Advances in Life Sciences, 5(8): 2999-3007 (NASS Rating 3.56) Rajesh Kr Gupta, Madhu Gupta, P. Ray Choudhury, and Asit B. Mandal (2016). Identification and classification of Scented and non Scented rice cultivars based on DNA fingerprinting. Advances in Life Sciences 5(7): 2917-2925 (NASS Rating 3.56) Rajesh Kr Gupta, Dipti Kamal, P. Ray Choudhury, and Asit B. Mandal (2016). Genetic Diversity Among Heat Tolerant and Susceptible Wheat (Triticum aestivum L.) Cultivars by Employing RAPD Markers. Advances in Life Sciences, 5(7): 2824-2830 (NASS Rating 3.56) Avinash Pathak, Rajesh Kr. Gupta, P. Ray Choudhury, and Asit B. Mandal (2015) Transferability of Rice Microsatellite Markers (STMS) Across Cool Season Crops. Trends in Biosciences, 8(22): 6217-6221(NASS Rating 2.74) Page 2
- 3. CV: Rajesh Kumar Gupta Rajesh K Gupta, Madhu Gupta, P. Ray Choudhury, and Asit B. Mandal (2015) Detection of DNA polymorphism among rice landraces and cultivars using STMS and RAPD markers. Trends in Biosciences, 8(24): 6800-6807 (NASS Rating 2.74) International/National Conferences Abstracts R.K. Gupta, Monika Dalal, S.K. Sinha, G. Thomas P.R. Choudhury, A.K. Ahlawat, and A. Mahendru Singh3 and P.K. Mandal (2015). A modified protocol for sequential extraction of gliadins, glutenins and other proteins from wheat seed. In: Society for Plant Biochemistry and Biotechnology (SPBB) organized a National Symposium on Germplasm to Genes: Harnessing Biotechnology for Food Security and Health during 9-11 August, 2015 at NAAS Complex, New Delhi R.K. Gupta, Monika Dalal, S.K. Sinha, G. Thomas, A. Mahendru, P.R. Choudhury and P.K. Mandal (2014). Celiac Disease: Wheat Gliadin Protein as a Key Player. In: SCIENCE OF OMICS FOR AGRICULTURAL PRODUCTIVITY : FUTURE PERSPECTIVES, March 4 – 6, 2014 organized by Dept of Biotechnology, Pantnagar University, UK. India. Subodh Kumar Sinha, Niketa Bansal, Gaytri, Rajesh Gupta and PK Mandal (2013). Genotype variation of wheat seedling in response to low N condition on growth under hydroponics. In: International conference on role of plant biochemistry and technology in food and nutritional security dated December 11-14, 2013 organized by Dept of Biochemistry, Sri Venkateswara University, Tirupati, 517502 AP, India Gupta, R.K., P. Ray Choudhury and Asit B. Mandal (2009) Cross species transferability of rice microsatellite markers in major crops. In: International Conference on Emerging Trends in Biotechnology (ETBT-2009) and 6th Annual Convention of The Biotech Research Society, India (BRSI). Organized by: BHU and IIVR (Varanasi); Dec 4-6.2009.Published. Gupta, R.K., Choudhury, P. Ray and Mandal, Asit. B (2010) Detection of polymorphic SSR loci among landraces grown in northern part and some elite rice varieties. In: National Conference of Plant Physiology on “Physiological and Molecular Approaches for crop Improvement under Changing Environment” November 25-27, 2010 at Banaras Hindu University, Varanasi, India. Published. Gupta R. K., pathak, A. K., Choudhury, P. Ray, Dhandapani, R and Mandal, Asit. B (2011) Molecular analysis among scented, non scented and rice landraces of eastern U.P by using SSR marker. In: National Seed Congress on Quality Seeds for prosperity-28-30 january,2011,College of Agriculture, Pune (M.S) India, organized by National Seed Research and Training Centre Varanasi and Mahatma Phule Krishi Vidyapeeth, Rahuri, Dist. Ahmednagar, Maharashtra. Published. Pathak, A. K., Gupta R. K., Choudhury, P. Ray, Dhandapani, R and Mandal, Asit. B (2011) Transferability of Rice Microsatellite Markers in Cool Season Crop. In: National Seed Congress on Quality Seeds for prosperity-28-30 january, 2011,College of Agriculture, Pune (M.S) India, organized by National Seed Research and Training Centre Varanasi and Mahatma Phule Krishi Vidyapeeth, Rahuri, Dist. Ahmednagar, Maharashtra. Published. Page 3
- 4. CV: Rajesh Kumar Gupta Gupta, R. K., P. Ray Choudhury and G.Thomas. (2011). Identification of polymorphic SSR loci among landraces and some elite rice varieties. In: ”National Symposium On Biodiversity & Food Security-Challenges & Devising Strategies December 10-11, 2011 organized by Society for Advancement of Science & Rural Development and Indian Institute of Pulses Research, Kanpur -208024. Published Gupta, R. K., P. Ray Choudhury and G. Thomas (2012). Studied of genetic diversity between scented and non scented rice includind landraces using ISSR Markrs. In: International conference on mycology and plant pathology biotechnological approaches-2012, Feb 27-29, 2012 organized by centre of advanced study in botany, BHU, Varanasi-221005. Published Participation in workshop and Training Participated in workshop on “Sustainable Agriculture: Harnessing Technologies- Harvesting Prosperity”29 January, 2016 at FICCI, New Delhi Participated in workshop on “Safe use of unseal radiation source” at the National Research Laboratory, IARI, New Delhi from 11-12 march 2014. Participated in workshop on “recent trends in Bioinformatics, System Biology & Biomolecular interaction” held at center of bioinformatics, University of Allahabad from 8-10 January 2012. Participated in Training program on “computational tool & techniques for predicting immunogenicity” at The Bioinformatica Solution, Lucknow from 10 Aug-10 Sep 2012. Directorate of Seed Research (ICAR), Mau, conducted a Model Training course on “Molecular approaches for genetic purity of seeds” in collaboration with directorate of extension, Government of India, New Delhi, duration: 7-14 February, 2010. Research Experience (A): InIn ICAR- NRC on Plant Biotechnology, IARI, April 2012- till date working as a Ph. D Scholar in molecular biology lab involving in project “modification of wheat gluten proteins responsible for allergy in human. (B): In ICAR-DSR, Aug 2010-April 2011, worked on in-house project “Assessment of Genetic Purity of Major Cereals Crops (Rice, Wheat & Soybean) and Hybrids (Rice & Cotton) through Molecular Tools & Techniques” We have identified two reproducible marker for quick diagnostics of genetic and Rice Hybrids purity (PSD-3, DRRH-2 and DRRH-3) and their parental line, two reproducible marker for Cotton Hybrids (CSHH 238 &CSHH 198) with SSR marker. (C): In ICAR-DSR, Three months dissertation for M.Sc. Thesis entitled “search of common microsatellite markers that show transferability in major crops”. We focused on Highly polymorphic Page 4
- 5. CV: Rajesh Kumar Gupta and transferable molecular markers in oryza can be useful for exploiting the genetic resources of this genus and detecting allelic variants in loci associated with other important crops. Area of interest Molecular Biology Functional Genomics Structural Biology Transcriptomics & Metabolomics Personal Information Fathers’ Name- Bhola Gupta Mother’s Name – Champa Devi Date of Birth: 25/07/1984 Sex: Male Nationality: Indian Marital Status: Married Permanent Address: Vill + Post – Deochandpur. Distt. – Ghazipur Pin Code-233304, U.P., India Mobile-09415222899 (Home) Corresponding Address: WZ-277A, Inderpuri, Behind KB’s Fairprice, New Delhi - 110012, India. Contact Information: Mb: 91-9818602532; 91-9868656031 E-Mail: rajesh.raj.25784@gmail.com dr.rajeshbt@rediffmail.com Membership Life time member of The Indian Science Congress Association (L23569). Life time member of Society for Plant biochemistry and biotechnology (L727). Page 5
- 6. CV: Rajesh Kumar Gupta References Dr. P. K. Mandal Principal scientist Lab No. 221, NRCPB, IARI, Pusa, New Delhi. pranabkumarmandal@gmail.com Prof. (Dr.) George Thomas Head of Department, Department of Molecular and Cellular Engineering SHIATS, (Allahabad Agriculture Institute, Deemed University) Allahabad, UP, India. Mobile: 09935749711 E mail-gthomas61@yahoo.com Dr. P. Ray Choudhury Principal Scientist (Plant Biotech.) Crop Science Division ICAR Head Quarters, New Delhi Mobile: 08826549971 Email-prc71@rediffmail.com Declaration I confirm that the information provided by me is true to the best of my knowledge and belief. Place: New Delhi (Rajesh Kr. Gupta) Date: 15.08.2016 Page 6
- 7. CV: Rajesh Kumar Gupta References Dr. P. K. Mandal Principal scientist Lab No. 221, NRCPB, IARI, Pusa, New Delhi. pranabkumarmandal@gmail.com Prof. (Dr.) George Thomas Head of Department, Department of Molecular and Cellular Engineering SHIATS, (Allahabad Agriculture Institute, Deemed University) Allahabad, UP, India. Mobile: 09935749711 E mail-gthomas61@yahoo.com Dr. P. Ray Choudhury Principal Scientist (Plant Biotech.) Crop Science Division ICAR Head Quarters, New Delhi Mobile: 08826549971 Email-prc71@rediffmail.com Declaration I confirm that the information provided by me is true to the best of my knowledge and belief. Place: New Delhi (Rajesh Kr. Gupta) Date: 15.08.2016 Page 6