Recombinant DNA technology
Download as PPTX, PDF7 likes30,966 views
This document discusses diabetes and how recombinant DNA technology can be used to produce insulin. It begins by outlining what diabetes is and how insulin regulates blood glucose levels. It then explains that recombinant DNA involves combining DNA from different species and inserting it into a host organism, like E. coli bacteria, to produce useful genetic combinations. The key steps are isolating the human insulin gene, inserting it into a bacterial plasmid using DNA-modifying enzymes and ligase, and transforming the bacteria so it can reproduce and produce large quantities of insulin.
Related topics:1 of 13
Downloaded 840 times
More Related Content
What's hot (20)
Ad
Viewers also liked (19)
Ad
Similar to Recombinant DNA technology (20)
Recombinant DNA technology
- 2. Today we willUnderstand what Diabetes is and how it affects the personUnderstand how insulin can be produced by recombinant DNA technology
- 3. The problem - DiabetesWhat do you know?Symptoms? Cause? Treatment?
- 4. Insulin– what is it exactly?A hormone (chemical messenger) that allows glucose to pass from the blood into your cells.
- 5. How it works
- 6. The solution - how can we getb lots of insulin?Other animals?Get something less cute to make it for us using the gene for insulin.
- 7. Recombinant DNA technologyJoining together DNA molecules from two different species. This is then inserted into a host organism which can produce new genetic combinations that are useful to us
- 9. Bacteria – E. coliTransformation
- 10. And this is how…
- 11. Key wordsIsolated gene Sticky ends Vector Plasmid Restriction enzyme DNA ligaseRecombinant plasmidTransformationSteps (not in order)The insulin gene is inserted into the bacterial plasmid using DNA ligaseThe gene for insulin is isolated andremoved from human DNA leaving it with sticky endsThe recombinant plasmid is taken up by the bacteria by transformationA plasmid from the bacteria E coli is removed and cut open using restriction enzymesThe bacteria reproduces, making copies of the gene each time, allowing lots of insulin to be produced
- 12. What else?