reference from INSERM DOMINIQUE
- 1. University of Burgundy Inserm Research Center U866 “Lipids, Nutrition, Cancers” “Chemotherapy, Lipid Metabolism and Antitumoral Immune Response ” Team 7 bd Jeanne d’Arc, 2100 Dijon, France Pr. Dominique DELMAS Tel 33 3 80 39 32 26 – Fax 33 3 80 39 34 34 E-mail: ddelmas@u-bourgogne.fr Dijon, June 11, 2015 Nicholas Nicou is a Bachelor level student who came to France performed a 9-month internship as Erasmus in the INSERM research center in Dijon. I welcomed this student in my research group working on tumoral resistance and the link between lipid metabolism abnormalities and the emergence of chemotherapy resistance. The research subject of his dissertation dealt with the effects of various anticancer drugs on the lipid metabolism in chemoresistant tumoral cells. His work began with studies on the effects of these anticancer drugs on various colon cancer cells that present different sensitivities. During this training, Nicholas has used different techniques such as cytotoxic tests (crystal violet) or apoptotic test by flux cytometry (7AAD/Annexin V). In a second part, he analyzed the impact of chemotherapeutic drug on lipid metabolism especially on phospholipid biosynthesis. This work required a molecular biology approach particularly immunoblotting experiments. Finally, he analyzed by immunofluorescence the content of of lipid in tumoral cells. During his training, I particularly appreciated the motivation and the work capacities of Nicholas. He conducted all the above described works with intellectual curiosity and enthusiasm. He is a conscientious and a pleasant man and integrated without difficulties in our research team. Pr. Dominique Delmas