RFLPs (Restriction Fragment Length Polymerase).pdf
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Restriction fragment length polymorphism (RFLP) involves genetic markers from variations in DNA fragment lengths produced by restriction endonuclease digestion. Advantages include co-dominance and widespread applicability, while disadvantages include labor intensity and time consumption. RFLPs are part of a larger set of DNA markers, including hybridization and PCR-based markers.
RFLPs (Restriction Fragment Length Polymerase).pdf
- 2. • Genetic markers resulting from the variation or change in the length of defined DNA fragments produced by digestion of the DNA sample with restriction endonucleases. • Use: Southern blots
- 6. Advantages • Variants are Co-dominant • Measures variation at the level of DNA sequence, not protein sequence • Ubiquitous ( large number of markers , probe –enzyme combinations .) Disadvantages • Labor intensive • Requires relatively large amounts of DNA • Time consuming .
- 7. DNA markers Hybridization based markers RFLPs Minisatellites Microsatellites PCR-based markers RAPDs MP-PCR AFLPs SSRs SCoT