Rna splicing
- 2. • the removal of introns from a primary transcript and the subsequent joining of exons in the production of a mature RNA molecule. RNA splicing transcriptio n exons intron s Rna splicing translation DNA PRE- MRNA MATURED RNA Polypeptid e
- 3. history phillip Allen Sharp is an American geneticist and molecular biologist who co-discovered RNA splicing. He shared the 1993 Nobel Prize with Richard J. Roberts for "the discovery that genes in eukaryotes are not contiguous strings but contain introns, and that the splicing of messenger RNA to delete those introns can occur in different ways, yielding different proteins from the same DNA sequence". phillip Allen Sharp Richard J. Roberts
- 4. 1 2 4 5 6 8 9 103 7 1 2 5 7 6 8 9 104 51 4 6 4
- 5. 1 2 4 5 6 8 9 103 7 1 4 52 1 4 6 7 64 8 9 105 1 2 5 7 6 8 9 104 51 4 6 4 1 4 52 1 4 6 7 64 8 9 105
- 7. BREAKING PHOSPHODIESTE R BOND DUE TO ADDITION OF –OH GROUP FORMATION OF PHOSPHODIESTE R BOND – formation of loop
- 8. but what would help to do these Spliceoso me
- 9. • It is a large and complex molecular machine which helps to remove the introns from pre-Mrna molecule Spliceosome ●The complexes of snRNA and proteins are called small nuclear ribonuclear proteins (snRNP, pronounces “snurps”). There are 5 snrnp’s U1, U2, U4, U5, U6 ● Spliceosome act by formation of- COMPLEX E COMPLEX A COMPLEX B COMPLEX C
- 10. u1 u2 u4 u5 u6
- 11. E complex a complex a complex b complex b complex c complex u1 bbp u2af65 u2af36 u1 u1 u1 u1 bbp u1 u2 u2 u2af65 u2af36 u2 U6, u4, u5 u4 U6, u4, u5 u2
- 12. Rna splicing Introduction Why intron are important process Spliceosome Complex
- 13. Reference http://genetics.thetech.org/ask/ask326 http://www2.oakland.edu/biology/chaudhry/pics/chapter24f.pdf http://www.dnalc.org/resources/animations/rna-splicing.html http://highered.mheducation.com/olcweb/cgi/pluginpop.cgi?it=swf::535::535::/site s/dl/free/0072437316/120077/bio30.swf::How+Spliceosomes+Process+RNA http://www.evolutionnews.org/2013/09/the_spliceosome_1076371.html http://www.evolutionnews.org/2011/12/finding_treasur054241.html