RP-HPLC Method Development and Validation for the Simultaneous Estimation of Metoprolol and Telmisartan in Tablet Dosage Form

75
* Corresponding author: K.Pranitha
E-mail address: kankanalapranitha@gmail.com
IJPAR |Volume 3 | Issue 1 | Jan-Mar-2014 ISSN: 2320-2831
Available Online at: www.ijpar.com
[Research article]
RP-HPLC Method Development and Validation for the
Simultaneous Estimation of Metoprolol and Telmisartan
in Tablet Dosage Form
*K.Pranitha, Tabassum, J.Priya, N.Ramathilagam.
Department of Pharmaceutical Analysis and Quality Assurance, Smt.Sarojini Ramulamma
College of Pharmacy, Sheshadrinagar, Mahabubnagar - 509001, Andhrapradesh, India.
ABSTRACT
A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and
validated for simultaneous determination of Metoprolol and Telmisartan in pharmaceutical tablet dosage form.
Chromatographic analysis was performed on a Symmetry X-terra C8 (4.6 mm x 100 mm, 5 m) column at
ambient temperature with a mixture of ortho phosphoric acid buffer and Acetonitrile, Methanol in the ratio
45:10:45 v/v as mobile phase, at a flow rate of 0.7 mL min-1
. UV detection was performed at 226 nm. The
retention times of Metoprolol and Telmisartan were 2.473 and 3.407 min, respectively. The correlation
coefficient of Metoprolol and Telmisartan was found to be 0.999. Calibration plots were linear over the
concentration ranges 12.5–62.5 μg mL-1
and 10-50 μg mL-1
for Metoprolol and Telmisartan, respectively. The
Limit of detection was 0.667 and 0.846µg mL-1
and the quantification limit was 2.021 µg mL-1
and 2.565 µg
mL-1
for Metoprolol and Telmisartan, respectively. The accuracy of the proposed method was determined by
recovery studies and found to be 99.93% to 101.09%.The method was validated for accuracy, linearity,
sensitivity, precision, robustness, system suitability Commercial tablet formulation was successfully analyzed
using the developed method and the proposed method is applicable to routine analysis of determination of
Metoprolol and Telmisartan in pharmaceutical tablet dosage form.
Keywords: Metoprolol, Telmisartan, RP-HPLC, Validation.
INTRODUCTION
Metoprolol is chemically described as: (RS)-1-
(Isopropyl amino)-3-[4-(2-methoxyethyl) phenoxy]
propan-2-ol. It is used mainly in treatment of
several diseases of the cardiovascular system,
especially hypertension it is a beta-adrenergic
receptor blocking agent. Telmisartan is chemically
described as: 2-(4-{[4-methyl-6-(1-methyl-1 H-1,
3-benzodiazol-2-yl)-2-propyl-1H-1, 3-benzodiazol-
1-yl] methyl} phenyl) benzoic acid. It is used as
antihypertensive and orally active nonpeptide
angiotensin II antagonist that acts on the AT1
receptor subtype. Angiotensin II is the principal
precursor agent of the renin-angiotensin system,
with effects that include vasoconstriction,
stimulation of synthesis and release of aldosterone,
cardiac stimulation and renal reabsorption of
sodium. Fix dosage combination containing
Metoprolol (50 mg) and Telmisartan (40mg)
available in market. A new combination
formulation of metoprolol and Telmisartan seems
to be beneficial in the treatment and management

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K.Pranitha et al / Int. J. of Pharmacy and Analytical Research Vol-3(1) 2014 [75-82]
www.ijpar.com
of essential hypertension in terms of its
convenience and patient compliance. Literature
survey revealed HPLC8-10, HPTLC13, and
simultaneous UV spectrophotometric methods15
have been reported for the estimation of MET, TEL
either alone or in combination with other drugs like
Hydrochlorothiazide 10, Indapamide13, Ramipril
14, etc. The present research work describes the
rapid, accurate, sensitive and reproducible RP-
HPLC method for simultaneous estimation of
Metoprolol and Telmisartan from the tablet
formulation.
.
METOPROLOL TELMISARTAN
MATERIALS AND METHODS
Chemicals/ Reagents and Solvents
Metoprolol -50mg and Telmisartan-40mg were
obtained from, GLENMARK Pharmaceutical Ltd.
Mumbai. Double Distilled Water (HPLC grade),
Methanol (HPLC grade), Acetonitrile (HPLC
grade), orthophosphoric acid and Potassium-
dihydrogen phosphate were of reagent grade. The
pharmaceutical preparations of combination of
Metoprolol and Telmisartan that is TELMAXX 50
tablet (GLENMARK Pharmaceutical Ltd.
Mumbai.).
Instrumentation and Equipments
The HPLC analysis was accomplished on
WATERS high pressure liquid chromatography
outfitted with 515 reciprocating dual column HPLC
pump, a manually operating Rheodyne injector
with 20 μL sample loop, X-terra C8 4.6 mm x 150
mm analytical column reversed-phase material of
5μ size and a 2487 model UV-Visible detector. All
the parameters of HPLC were controlled by N 2000
chromatographic system software. Other
instruments used were TECHCOMP UV-Vis
spectrophotometer of model 2310, Shimadzu
electronic balance of model XEX-200, ADWA of
model AD102U digital pH meter and ENERTECH
of model SE60US ultrasonic bath sonicator.
ANALYTICAL METHOD
DEVELOPMENT
Optimization of UV conditions
A symmetry X-terra C8(4.6mm x150mm,5m) was
used for chromatographic separation. The mobile
phase composed of pH 3 Buffer (Ortho phosphoric
acid):Acetonitrile:Methanol (45:10:45) at flow rate
0.7 mL/min with run time 6 mins. Mobile phase
and sample solution were filtered through a 0.45
μm membrane filter and degassed. The detection of
both drugs was carried out at 226 nm.
Figure-1. Isobestic point of Metoprolol and Telmisartan.

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Optimized Method Parameters
MobilePhase : Phosphate buffer (3.0 pH): Acetonitrile: Methanol (45:10:45)
Column (Stationary Phase): X-terra (C8) (4.6mm x 150mm, 5m)
Flow rate (ml/min): 0.7
Column temperature (°C): Ambient
Volume of injection loop (l): 20
Detection wavelength (nm):226
Drug RT (min): Metoprolol- 2.4, Telmisartan- 3.4
Figure 1.1.Optimized chromatogram
Fig: 1 Optimized chromatogram
PROCEDURE FOR PREPARATION OF
SOLUTION
Preparation of buffer
Take 1000ml of HPLC grade water. Dissolve 2.72
grams of Potassium di hydrogen phosphate salt and
Adjusted the pH to 3.0 with orthophosphoric acid.
Preparation of mobile phase
A mixture of above prepared buffer 450 ml (45%),
450 ml of HPLC grade Methanol and 100 mL of
Acetonitrile (10%) were mixed and degassed in
ultrasonic water bath for 5 minutes. The mobile
phase was filterred through 0.45 µ filter under
vacuum.
Diluent Preparation
Use Mobile phase Diluent Phase
ASSAY
Preparation of the Metoprolol And
Telmisartan standard & sample solution
Preparation of Standard Solution
Accurately weighed and transferred 12.5 mg of
Metoprolol and 10 mg of Telmisartan working
standard into a 100 ml clean dry volumetric flask
and added about 70 ml of diluent. It was sonicated
to dissolve completely and made volume up to the
mark with the same diluent. (Stock solution)
From this, 3 ml of the solution was pipetted into
another 10 ml volumetric flask and diluted up to
the mark with diluent.
Sample Solution Preparation
Accurately weighed and transferred tablet powder
equivalent to 12.5 mg of Metoprolol and 10 mg of
Telmisartan (136.3 mg) into a 100ml clean dry
volumetric flask and added about 70ml of diluent.
It was sonicated to dissolve completely and made
volume up to the mark with the same diluent.
(Stock solution)
From this, 3 ml of the solution was pipetted into
another 10 ml volumetric flask and diluted up to
the mark with diluent
Procedure
20 µL of the standard and sample solutions were
injected into the chromatographic system and areas
for the Metoprolol and Telmisartan peaks were
measured. % Assay was calculated by using the
formulae.
Calculation:
Assay % =
AT WS DT P Avg. Wt
--------- x -------- -x ------- x ------- x ------------- X 100
AS DS WT 100 Label Claim

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Where:
AT = Average area counts of sample preparation.
AS = Average area counts of standard
preparation.
WS = Weight of working standard taken in mg.
P = Percentage purity of working standard
LC = LABEL CLAIM mg/ml.
ANALYTICAL METHOD VALIDATION
The HPLC method was validated in accordance
with ICH guidelines.
Accuracy
Accuracy was carried out by % recovery studies at
three different concentration levels. To the pre-
analyzed sample solution of METO and TELM a
known amount of standard drug powder of METO
and TELM were added at 80, 100 and 120 % level.
Precision
The system precision of the method was verified by
five replicate injections of standard solution
containing METO and TELM. The method
precision was carried out the analyte five times
using the proposed method. Repeatability was
measured by multiple injections of a homogenous
sample of METO and TELM.
Linearity
The linearity was determined separately for METO
and TELM Linearity of the method was studied by
injecting 5 concentrations of both drugs prepared
in methanol and calibration curves were
constructed by plotting peak area against the
respective concentrations.
Limit of detection and Limit of quantitation
Sensitivity of the proposed method was estimated
in terms of Limit of Detection (LOD) and Limit of
Quantitation (LOQ). LOD = 3.3 x ASD/S and LOQ
= 10 x ASD/S, Where, ‘ASD’ is the average
standard deviation and ‘S’ is the slope of the line.
Robustness
Robustness was evaluated by making deliberate
variations in method parameters such as variation
of wave length; flow rate and change in mobile
phase composition. The robustness of the method
was studied for METO and TELM
RESULTS
Selection of Chromatographic Conditions
and Optimization of Mobile Phase
Mobile phase was optimized to separate METO
and TELM using Symmetry C8 column (150 mm x
4.6 mm i.d., 5μm). Initially, ACN and phosphate
buffer and methanol in the Equal proportions were
tried as mobile phase but the splitting of the peaks
for both these drugs was observed. Therefore, after
adjustment of pH of mixed phosphate buffer to 3.0
with ortho-phosphoric acid, and mobile phase
composition (phosphate buffer, ACN and methanol
in 45:10:45 % v/v) was tried for resolution of both
drugs. Good resolution and symmetric peaks were
obtained for both drugs when the pH of the mobile
phase (buffer) was adjusted to 3.0. The flow rate of
the mobile phase was 0.7 mL min-1. Under
optimum chromatographic conditions, the retention
time for METO and TELM was found to be 2.473
and 3.407 min, respectively when the detection was
carried out at 226nm. A typical chromatogram of
two drugs is shown in (Figure 1).
Table-1 : ACCURACY DATA
Drugs Amount Added
(mg)
Amount Found
(mg)
% Recovery % of mean recovery
Metoprolol
67.5 67.43 99.8
99.9375 76.3 102
82 81.55 98
Telmisartan
54 53.86 99.42
101.0960 60.41 101.37
66 66.90 102.50

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Table-2 : PRECISION
Table-3 METHOD PRECISION
S.NO RT AREA RT AREA
1
2.490 1723623 3.423 5925015
2
2.515 1709983 3.448 5864329
3
2.492 1720330 3.418 5891368
4
2.488 1723516 3.423 5924883
5
2.498 1720296 3.423 5909737
Average 2.4966 1719549.6 3.427 5903066
Standard Deviation 0.010 5590.41 0.011 25688.72
% RSD 0.4 0.33 0.32 0.44
Table-4: LINEARITY RESULTS OF METOPROLOL AND TELMISARTAN
METOPROLOL TELMISARTAN
Conc(mcg/ml) Mean Area Conc(mcg/ml) Mean Area
12.5 319799 10 1677519
25 1003211 20 3739380
37.5 1755032 30 6084698
50 2551909 40 8174726
62.5 3150848 50 10315646
S.NO RT METOPROLOL AREA RT TELMISARTAN AREA
1 2.472 1745269 3.397 6017053
2 2.543 1744223 3.477 6061793
3 2.498 1755032 3.440 6084698
4 2.473 1745274 3.398 6017163
5 2.548 1744271 3.482 6061885
Average 2.5068 1746614 3.4388 6048520
Standard Deviation 0.0368 4726.359 0.041 30151.48
% RSD 1.4 0.27 1.2 0.50

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Fig 2: LINEARITY GRAPHS OF METOPROLOL AND TELMISARTAN
Table-5 LOD AND LOQ RESULTS
S.No Drug name Standard deviation Slope LOD LOQ
1 Metoprolol 11659 57686 0.66 2.02
2 Telmisartan 55707 217116 0.84 2.56
Table-6: ROBUSTNESS RESULTS
S.No
Parameter Metoprolol Telmisartan
Theoretical
plates per
column
Tailing
factor
Resolution Theoretical
plates per
column
Tailing
factor
Resolution
1 Less
flow(0.63ml/min)
2122 1.287 - 4101 1.139 5.462
2 More
flow(0.77ml/min)
2095 1.295 - 3952 1.149 5.326
3 %10 Less organic 2101 1.255 - 4028 1.134 5.404
4 %10 More organic 2042 1.245 - 3892 1.124 5.323
RESULTS AND DISCUSSION
Accuracy
The accuracy of the method studied at three
different concentration levels i.e. 80%, 100 % and
120 % showed acceptable % recoveries in the range
of 99.93% for METO and 101.09% for TELM. The
results are shown in Table 1.
Precision
The precision study was evaluated on the basis of
% RSD value was found to be in the range 0.27–
1.4 and 0.32-0.4 %, respectively. As the RSD
values were < 2% therefore developed method was
precise. Results of precision study are shown in
Table 2 & 3.

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Linearity
The linearity was determined separately for METO
and TELM. Linearity of the method was studied by
injecting 5 concentrations of both drugs prepared in
methanol and calibration curves were constructed
by plotting peak area against the respective
concentrations. The METO and TELM followed
linearity in the concentration range of 12.5– 62.5
μg mL-1 and 10-50μg mL-1; respectively. The
results are shown in Table 4.and Fig no 2.
Limit of detection and Limit of quantitation
The LOD for METO and TELM was found to be
0.667 and 0.846 μg, respectively. The LOQ for
METO and TELM was found to be 2.021 and
2.565 μg, respectively. The low values of LOD and
LOQ indicates high sensitivity of the method. The
results are shown in Table 5.
Robustness study
Robustness of the method was studied by making
deliberate changes in the chromatographic
conditions and the effects on the results were
examined. The low value changes of theoretical
plates, tailing factor indicating robustness of the
method. The results are shown in Table 6.
Analysis of marketed tablet formulation
3 replicates of the samples solutions (20 μL) were
injected for quantitative analysis. The amounts of
METO and TELM estimated were found to 99.09
% and 98.42 %, respectively. A good separation
and resolution of both drugs indicates that there
was no interference from the excipients commonly
present in pharmaceutical formulations. The results
are shown in Table 7.
System Suitability Test
The system suitability parameters such as
resolution, number of theoretical plates and tailing
factor were studied and were summarized in Table
8.
Table 7: ASSAY RESULTS
Assay Results Drug Amount present/tablet % of Assay
METOPROLOL 50mg 99.09
TELMISARTAN 40 mg 98.42
Table 8: SYSTEM SUITABILITY PARAMETERS
System suitability Parameters METOPROLOL TELMISARTAN
Tailing Factor 1.275 1.145
Theoritical plates 2076 3997
Resolution -- 5.381
CONCLUSION
The developed RP-HPLC method is simple,
precise, accurate, selective and reproducible. The
method has been found to be adequately in linearty
and robustness can be used for simultaneous
determination of Metoprolol and Telmisartan in
tablet formulation. The method was validated as
per ICH guidelines.
ACKNOWLEDGEMENT
I like to thankful to Pharm Tech Solution,
Cherlapally, Hyderabad, India for providing the
gift samples of Metoprolol and Telmisartan. And
also to the principal Dr.K.Rajeswar Dutt
Smt.Sarojini Ramulamma College of Pharmacy,
Mahabubnagar , Andhrapradesh and special thanks
for Mrs.J.PRIYA Madam & Ms.Ramathilagam
madam as well as my friends who helped during
the project work.
REFERENCES
[1] British Pharmacopoeia 2010, London-HMSO Publication. Vol II p.1419,1042.

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[2] Brirtish Pharmacopoeia: Medicines and Healthcare products Regulatory Agency (MHRA): Telmisartan:
5872
[3] Indian Pharmacopoeia 2010, Government of India, Ministry Of Health and Welfare. Published by Indian
Pharmacopoeia commission, 2010, 1681-1682, 2186-2187.
[4] The United States Pharmacopoeia: London: The United States Pharmacopoeial Convention: Metoprolol:
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[5] Rang H.P, Dale M.M, Ritter J.N,Moore P.K,Pharmacology, 5th edn, New york, Churchill, Livingstone,
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[6] Laurence LB, Goodman and Gilman: The pharmacological basis of therapeutics:11th ed.: Mcgraw-hill
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[7] Martindale The Complete Drug Reference: Telmisartan: 951.3.
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[12]Prajakta S.Nawle et al; proposed normal and reverse phase HPTLC methods for simultaneous estimation of
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Telmisartan and Indapamide by RP-HPLC in pure and marketed formulations. Int J Pharm And Biomed
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RP-HPLC Method Development and Validation for the Simultaneous Estimation of Metoprolol and Telmisartan in Tablet Dosage Form

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RP-HPLC Method Development and Validation for the Simultaneous Estimation of Metoprolol and Telmisartan in Tablet Dosage Form