Shedding Light on Optogenetics
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The document discusses optogenetics, a method of manipulating neurons using light and genetically modified cells. It outlines its historical context, advantages like specificity and speed, and applications in neuroscience, particularly for pain relief and studying fear responses. A bibliography of relevant studies and reviews is also included.
![Historical Methods of Neural Manipulation
● Lesioning
● Genetic Modification
● Physical Stimulation
● Electrical Stimulation
● Drugs
[2] H-Y. Lai et al., 2012
Specificity?
Invasiveness?
Duration? Speed?](https://image.slidesharecdn.com/raywardoptogenetics-190610194019/85/Shedding-Light-on-Optogenetics-2-320.jpg)
![Introducing Optogenetics
Cells genetically modified to respond to light
Specificity?
Invasiveness?
Duration? Speed?
[3] S. B. Goncalves
[4] Deisseroth, 2010](https://image.slidesharecdn.com/raywardoptogenetics-190610194019/85/Shedding-Light-on-Optogenetics-3-320.jpg)
![How it Works
[1] Buchen, 2010](https://image.slidesharecdn.com/raywardoptogenetics-190610194019/85/Shedding-Light-on-Optogenetics-4-320.jpg)
![Introducing Optogenetics
Cells genetically modified to respond to light
Specificity?
Invasiveness?
Duration? Speed?
[3] Buchen, 2017
[4] Deisseroth, 2010](https://image.slidesharecdn.com/raywardoptogenetics-190610194019/85/Shedding-Light-on-Optogenetics-5-320.jpg)
![Optogenetics in Neuroscience
Images and Video by Karl Deisseroth
[1] Buchen, 2010](https://image.slidesharecdn.com/raywardoptogenetics-190610194019/85/Shedding-Light-on-Optogenetics-6-320.jpg)
![Applications of Neurogenetics
Epidural Optogenetics for
Analgesia
Genetic dissection of an amygdala
microcircuit that gates conditioned fear
[5] W. Haubensak
[6] R. Bonin, 2016](https://image.slidesharecdn.com/raywardoptogenetics-190610194019/85/Shedding-Light-on-Optogenetics-7-320.jpg)
![Bibliography
[1] L. Buchen “Neuroscience: Illuminating the Brain” Nature vol. 465, 26-28, MAY 5, 2010
[2] H-Y. Lai, L-D Liao, C-T. Lin, et al. “Design, simulation and experimental validation of a novel flexible neural probe for deep brain stimulation and
multichannel recording.“ Journal of Neural Engineering, vol. 9, APR 10, 2012
[3] S. B. Goncalves, J. F. Ribeiro, A. F. Silva, et al. “Design and Manufacturing Challenges of Optogenetics Neural Interfaces: A Review” Journal of Neural
Engineering, vol. 14.4, MAY 19, 2017
[4] K. Deisseroth “Optogenetics” Nature: Methods, vol. 8, 26-29, DEC 20, 2010
[5] W. Haubensak, P. Kunwar, H. Cai, et al. “Genetic dissection of an amygdala microcircuit that gates conditioned fear” Nature vol. 468, 270-276, NOV 11,
2010
[6] R. Bonin, F. Wang, M. Desrochers-Couture, et al. “Epidural optogenetics for controlled analgesia” Molecular Pain vol. 12, 1-11, MAR 9, 2016](https://image.slidesharecdn.com/raywardoptogenetics-190610194019/85/Shedding-Light-on-Optogenetics-8-320.jpg)
Shedding Light on Optogenetics
- 1. OptogeneticsShedding Light on Optogenetics Ray Ward
- 2. Historical Methods of Neural Manipulation ● Lesioning ● Genetic Modification ● Physical Stimulation ● Electrical Stimulation ● Drugs [2] H-Y. Lai et al., 2012 Specificity? Invasiveness? Duration? Speed?
- 3. Introducing Optogenetics Cells genetically modified to respond to light Specificity? Invasiveness? Duration? Speed? [3] S. B. Goncalves [4] Deisseroth, 2010
- 4. How it Works [1] Buchen, 2010
- 5. Introducing Optogenetics Cells genetically modified to respond to light Specificity? Invasiveness? Duration? Speed? [3] Buchen, 2017 [4] Deisseroth, 2010
- 6. Optogenetics in Neuroscience Images and Video by Karl Deisseroth [1] Buchen, 2010
- 7. Applications of Neurogenetics Epidural Optogenetics for Analgesia Genetic dissection of an amygdala microcircuit that gates conditioned fear [5] W. Haubensak [6] R. Bonin, 2016
- 8. Bibliography [1] L. Buchen “Neuroscience: Illuminating the Brain” Nature vol. 465, 26-28, MAY 5, 2010 [2] H-Y. Lai, L-D Liao, C-T. Lin, et al. “Design, simulation and experimental validation of a novel flexible neural probe for deep brain stimulation and multichannel recording.“ Journal of Neural Engineering, vol. 9, APR 10, 2012 [3] S. B. Goncalves, J. F. Ribeiro, A. F. Silva, et al. “Design and Manufacturing Challenges of Optogenetics Neural Interfaces: A Review” Journal of Neural Engineering, vol. 14.4, MAY 19, 2017 [4] K. Deisseroth “Optogenetics” Nature: Methods, vol. 8, 26-29, DEC 20, 2010 [5] W. Haubensak, P. Kunwar, H. Cai, et al. “Genetic dissection of an amygdala microcircuit that gates conditioned fear” Nature vol. 468, 270-276, NOV 11, 2010 [6] R. Bonin, F. Wang, M. Desrochers-Couture, et al. “Epidural optogenetics for controlled analgesia” Molecular Pain vol. 12, 1-11, MAR 9, 2016