Stock solution calculation
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To prepare 300ml of 1x TAE buffer solution from a 50x stock solution, calculate the volume of stock solution needed using the formula C1V1=C2V2. Plugging in 50x for C1, 1x for C2, 300ml for V2 gives a value of 6ml for V1, the volume of 50x stock solution required. Take 6ml of the 50x TAE stock solution and dilute it with deionized water to a total volume of 300ml to obtain the desired 1x TAE buffer solution.
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Stock solution calculation
- 1. STOCK SOLUTION CALCULATION Dr. S. SIVASANKARA NARAYANI
- 2. FORMULA • C1V1 = C2V2 C1 = Concentration of the stock solution C2 = Concentration of Working solution V = Volume
- 3. PROB • You are provided with 50X TAE Buffer. From that have to prepare 1x TAE for 300ml. How?!!
- 4. CALCULATION C1*V1 = C2*V2 50x * V1 = 1X * 300ml V1 = 1X * 300/50x V1 = 6 ml
- 5. RESULT • Have to take 6 ml of 50xTAE and make up the volume till 300 ml with Deionized water