Study of Biosurfactant

COMPARATIVE STUDY OF BACTERIA
FOR BIOSURFACTANT PRODUCTION
USING DIFFERENT CARBON SOURCES
BY:
-ABHISHEK M. SIRSIKAR
-ANSHUL KUMAR GUPTA
-SAMEER S. HADAWALE
GUIDED BY:
DR.RAJENDRAN N.
VIT UNIVERSITY, VELLORE

INTRODUCTION
• Biosurfactants are microbially produced surface
active compounds. They are amphiphilic molecules
with both hydrophilic and hydrophobic regions.
• Biosurfactants possess different chemical
structures-lipopeptides, glycolipids, neutral lipids
and fatty acids.
• They are nontoxic biomolecules that are
biodegradable.

• Environment is generally known to be
oligotrophic in nature .
• Concentration and characteristics of carbon
sources in environment are variable and they
may influence the production of biosurfatctants.
• Therefore there is a need to check the influence
of different carbon sources on biosurfactant
production by bacteria.

OBJECTIVES
To study the influence of different
concentrations of various carbon sources on
bacterial growth and biosurfactant production.
To do comparative study between bacterial
strains for biosurfactant production.

MATERIALS AND METHODS
 Sample collection and isolation of microorganisms
 Soil sample collection,
 Serial dilution,
 Morphological study,
 Strain1 and Strain2.
 Preparation of medium using different carbon sources
 Carbon sources used - Dextrose, peptone, yeast and beef.
 Concentrations used -1.5%,1.0% and 0.5 %.
 Qualitative tests performed
- Haemolytic activity
- Oil displacement
- Emulsification index
- Biosurfactant yield

RESULTS AND CONCLUSION
0
0.5
1
1.5
2
2.5
Dextrose Peptone Yeast Beef
Oildisplacementindex(cm)
Carbon sources
1.5%
1.0%
0.5%
Fig.1.Comparison of oil displacement index
with different carbon sources at different
concentrations of Strain1
Strain 1
0
10
20
30
Emulsificationindex(%) Carbon sources
1.5%
1.0%
0.5%
Fig.2.Comparion of emulsification index

0
0.4
0.8
1.2
1.6
Haemolyticactivity(cm)
Carbon sources
1.5%
1.0%
0.5%
Fig.3.Comparison of haemolytic activity for
different carbon sources in different
0
50
100
150
200
250
300
350
400
450
Biosurfactantyield(mg)
Carbon sources
1.5%
1.0%
0.5%
Fig.4.Comparison of biosurfactant yield
Strain 1

0
0.2
0.4
0.6
0.8
Carbon sources
1.5%
0.5%
0
10
20
30
Emulsificationindex(%) Carbon sources
1.5%
0.5%
Fig.6. Comparison of emulsification index
for different carbon sources in different
concentrations of Strain 2
Fig.5. Comparison of oil displacement activity
with different carbon sources in different
concentrations of Strain 2
Strain 2

Strain 2
0
50
100
150
200
Carbon sources
1.5%
0.5%
Fig.6. Comparison of haemolytic activity
concentrations of strain 2
Fig.8.Comparison of biosurfactant yield
concentrations of strain 2
0
50
100
150
200
Carbon sources
1.5%
0.5%

Comparison between
Strain 1 and Strain 2

0
0.5
1
1.5
2
2.5
Carbon sources
1.5%
0.5%
0
0.5
1
1.5
2
2.5
Carbon sources
1.5%
0.5%
Emulsification index
0
10
20
30
Emulsificationindex(%)
Carbon sources
1.5%
0.5%Strain1
0
10
20
30
Emulsificationindex(%)
Carbon sources
1.5%
0.5%
Oil displacement
Strain1 Strain2
Strain2

Biosurfactant yield
0
50
100
150
200
250
300
350
400
450
Carbon sources
1.5%
0.5%
0
50
100
150
200
250
300
350
400
450
Dextrose Peptone Yeast BeefBiosurfactantyield(mg)
Carbon sources
1.5%
0.5%
Strain1 Strain 2

DISCUSSION
As the concentration of carbon-sources increased, the
production of biosurfactant increased indicating that increase
in concentration of carbon source (from 0.5% to 1.5%) might
have favored the production of biosurfactant.
Dextrose showed maximum biosurfactant yield, suggesting
that bacteria grow more effectively in the presence of
dextrose and thus biosurfactant production was also high as
compared to other carbon sources.
Strain 1 showed good biosurfactant production as compared
to strain 2.

Acknowledgment
We sincerely thanks to the honorable Dr. G. Viswanathan, VIT
University chancellor, for allowing us to carry out project in
VIT campus. Special thank to program chair person, our guide
and support staff for their much needed comments, help,
suggestion and support.

REFERENCES
[1] B.Anandraj,P.Thivakalan,Isolation and Production of Biosurfactant
producing organism from oil spilled soil, Biosci tech,vol.1(3),pp.120-126.
[2] N.Youssef, Comparison of methods to detect biosurfactant production by
diverse microorganisms, Journal of Microbiological methods 56(2004),
pp.339-347.
[3] S. Ray, Production of biosurfactant using an isolated bacterial strain of
Bacillus sp(m28),Journal of microbiology and Biotechnology
Research,vol.2,issue no 3,2012,pp.402-415.
[4].Vandanasingh, Biosurfactant-Isolation, Production, Purification
&significance, International Journal of scientific and Research Publications,
vol.2, Issue 7, July 2012.

[5].G.George,Sung-Chyr Lin, M.Sharma, Surface active compounds from
microorganisms, Austin: University of Texas.
[6].M.Bustamante, N.Duran, M.C.Diez, Biosurfactants are useful tools for
the bioremediation of contaminated soil: a review, Journal of soil science and
plant nutrition, vol.4 Temuco dic.2012.
[7].K. Bence, Bacterial production of antimicrobial biosurfactants by Bacillus
subtilis, Stellenbosch: Stellenbosch University, Dec 2011.
[8].V.Walten,C.Syldatk,R.Hausmann, Screening concepts for the isolation of
Biosurfactant producing microorganisms, Advances in Experimental
Medicine and Biology,vol.672,2010,pp.1-13.
[9].S. Viramontes-Ramos, et.al, selection of biosurfactant/bioemulsifier-
producing bacteria from hydrocarbon-contaminated soil, Brazilian Journal of
Microbiology, vol.41, oct.2010.

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