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Synergy™ 2 is a multi-mode microplate reader with performance,
speed and sensitivity. Synergy 2 challenges the more expensive
instruments, and is truly “best-in-class”, offering enhanced
sensitivity in fluorescence intensity, time-resolved fluorescence,
luminescence and UV-Vis absorbance and the addition of
fluorescence polarization.
Synergy 2 optimizes performance in all detection modes by using
dedicated optical elements for each individual technique. This
approach offers uncompromised performance and the lowest limits
of detection. Modular architecture provides for scalable instrument
options that result in acquisition cost savings and allow for future
upgrades. This combination of superior performance, flexibility and
cost-effectiveness makes Synergy 2 the best value in multi-detection
microplate readers.
Features:
• Detection Modes: Fluorescence intensity, time-resolved 		
	 fluorescence, fluorescence polarization, AlphaScreen®
, glow and 	
	 flash luminescence, UV-Vis absorbance, FRET, TR-FRET, BRET, area
scanning, spectral scanning
• Unique Optical System: A narrow band monochromator, 		
	 optimized filter/dichroic mirror pairing, and three broad spectrum 	
	 light sources achieve superior performance in all read modes
• High Sensitivity: Dedicated optical elements optimized for each 	
	 specific modality result in the lowest limits of detection available 	
	 in one compact instrument
• Modular Design: Purchase only what you need and upgrade 	
	 later as experimental requirements expand
• Compatible with Take3 plate with 2 µL microspots for low-volume 	
assays
• Wide Range of Applications: With built-in temperature control, 	
	 shaking, and optional dual reagent injector, virtually any 		
	 microplate-based application is possible
• Gen5™ Software: Integrates reader control, data analysis, 	
graphing and exporting tools in one powerful platform
	
Low-Volume Assays
Wide Range of Applications
	
Compatible with Take3™ plate with 2
µL microspots which enable low-volume
nucleic acid quantification.
Dual reagent injector for ion channel
assays, flash luminescence and fast
enzyme kinetics.
www.biotek.com
IRAN BioTek , Viragene Akam Co. Tel : +9821 88611552-3 Fax : +9821 88044577
Configurations:
Synergy 2: Detection systems and injectors available as individual
	 modules
See Web site or price list for configurations and descriptions.
Optional Accessories:
• Take3™ Micro-Volume Plate
• Absorbance Test Plate
• Fluorescence Test Plate
• Luminescence Test Plate
• Patented BioCell™ 1 cm quartz vessel
• Product Qualification Package
• Gen5™ Secure (for 21 CFR Part 11 Compliance)
BioTek Instruments, Inc.
Highland Park, P.O. Box 998
Winooski, Vermont 05404-0998, USA
Phone: 802-655-4040 • Toll-Free: 888-451-5171
Outside the USA: 802-655-4740
www.biotek.com
Gen5™ Data Analysis Software
The Synergy™ 2 features a software platform with a modern and
intuitive user interface.
Typical Applications:
• Nucleic acid quantification
• Protein quantification
• Enzyme kinetics
• Biomarker quantification
• ELISAs
• Genetic analysis
• Drug discovery
• Cell proliferation
• Cytotoxicity
• Drug absorption and metabolism
• Biologics drug discovery and development
• Food safety
• Biofuels research
• Environmental monitoring
AlphaScreen®
/AlphaLISA®
are registered trademarks of PerkinElmer.
Specifications:
General
Detection mode:	 FL, TRF, FP, Lum., UV-Vis Abs., AlphaScreen®
/AlphaLISA®
Read method:	 End point, kinetic, spectral scanning, well area scanning	
Microplate types:	 6 to 1536 wells (luminescence 1 to 384 wells)
		 PCR plates
		 Compatible with Take3™ Micro-Volume Plate	
Temperature control:	 4-Zone™ incubation from 4 °C above ambient to 65 °C;
			 ±0.2 °C at 37 °C
Shaking:	Yes
Software: 	 Gen5™ Data Analysis Software
Automation:	 Compatible with BioStack™ and 3rd party automation
AlphaScreen®
/AlphaLISA®
	 	
Light source:	 Tungsten halogen lamp
Sensitivity:	 100 amol of biotinylated-LCK-P peptide, 25 µL/well
			 in 384-well plate
Dynamic range:	 >6 decades
Detection system:	 Ultra low noise PMT
Absorbance 	 	
Light source:	 SQ xenon flash lamp
Wavelength selection:	 Monochromator
Wavelength range:	 200 – 999 nm, 1 nm increments
Bandpass: 	 2.4 nm
Dynamic range:	 0 – 4.0 OD
Resolution:	 0.0001 OD
Pathlength correction:	 Yes
OD accuracy:	 <1 % at 2.0 OD
		 <3% at 3.0 OD
OD repeatability:	 <0.5% at 2.0 OD
Reading speed:	 96 wells: 11 seconds
		 384 wells: 22 seconds
		 1536 wells: 42 seconds
Fluorescence Intensity 		
Sensitivity (SF):	 Top: 1 pM (0.2 fmol/well 96-well plate; 0.1 fmol/well
			 384-well plate)
		 Bottom: 5 pM (1 fmol/well 96-well plate; 0.5 fmol/well
			 384-well plate)
Light source:	 Tungsten halogen
		 High energy DPR xenon flash (optional)
Wavelength selection:	 Deep blocking bandpass filters/dichroic mirrors
Wavelength range:	 300 – 700 nm with tungsten lamp (850 nm option)
		 200 – 700 nm with xenon lamp (850 nm option)
Dynamic range: 	 >6 decades
Bandpass:	 Filter dependent
Detection system:	 PMT
Luminescence				 	
Sensitivity:	 10 amol ATP (flash)
Wavelength range:	 300 – 700 nm
Dynamic range: 	 >6 decades
detection system: 	 Low noise PMT	 	
Fluorescence Polarization 	 	
Light source:	 Tungsten halogen
		 High energy DPR xenon flash (optional)
Sensitivity:	 3 mP at 1 nM fluorescein
Wavelength selection:	 Deep blocking filters/dichroic mirrors
Wavelength range:	 400 – 700 nm (320 – 850 nm option)
	
Time-Resolved Fluorescence 	 	
Light source:	 High energy DPR xenon flash
Sensitivity:	 Europium 60 fM (12 amol/well 96-well plate; 6 amol/well
			 384-well plate)
Wavelength selection:	 Deep blocking filters/dichroic mirrors
Wavelength range:	 200 – 700 nm (850 nm option)
Reagent Dispensers 	 	
Number:	 2 syringe pumps
Dispense volume:	 5 – 1000 µL in 1 µL increments
Minimum prime volume:	 1.1 mL, 100 µL with back flush		
Physical Characteristics	 	
Power:	 100 – 240 Volts AC. 50/60 Hz
Dimensions:	 17”W x 17.5”D x 14.5”H (43.5 x 44.5 x 37.3 cm)
Weight: 	 60 lbs (27 kg)
Regulatory
For In Vitro Diagnostic use. CE and TUV marked, RoHS compliant.
Performance values represent the average observed factory test values.
*Specifications subject to change. Rev. 10/21/14
IRAN BioTek , Viragene Akam Co. Tel : +9821 88611552-3 Fax : +9821 88044577

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Synergy 2 single sheet english

  • 1. Synergy™ 2 is a multi-mode microplate reader with performance, speed and sensitivity. Synergy 2 challenges the more expensive instruments, and is truly “best-in-class”, offering enhanced sensitivity in fluorescence intensity, time-resolved fluorescence, luminescence and UV-Vis absorbance and the addition of fluorescence polarization. Synergy 2 optimizes performance in all detection modes by using dedicated optical elements for each individual technique. This approach offers uncompromised performance and the lowest limits of detection. Modular architecture provides for scalable instrument options that result in acquisition cost savings and allow for future upgrades. This combination of superior performance, flexibility and cost-effectiveness makes Synergy 2 the best value in multi-detection microplate readers. Features: • Detection Modes: Fluorescence intensity, time-resolved fluorescence, fluorescence polarization, AlphaScreen® , glow and flash luminescence, UV-Vis absorbance, FRET, TR-FRET, BRET, area scanning, spectral scanning • Unique Optical System: A narrow band monochromator, optimized filter/dichroic mirror pairing, and three broad spectrum light sources achieve superior performance in all read modes • High Sensitivity: Dedicated optical elements optimized for each specific modality result in the lowest limits of detection available in one compact instrument • Modular Design: Purchase only what you need and upgrade later as experimental requirements expand • Compatible with Take3 plate with 2 µL microspots for low-volume assays • Wide Range of Applications: With built-in temperature control, shaking, and optional dual reagent injector, virtually any microplate-based application is possible • Gen5™ Software: Integrates reader control, data analysis, graphing and exporting tools in one powerful platform Low-Volume Assays Wide Range of Applications Compatible with Take3™ plate with 2 µL microspots which enable low-volume nucleic acid quantification. Dual reagent injector for ion channel assays, flash luminescence and fast enzyme kinetics. www.biotek.com IRAN BioTek , Viragene Akam Co. Tel : +9821 88611552-3 Fax : +9821 88044577
  • 2. Configurations: Synergy 2: Detection systems and injectors available as individual modules See Web site or price list for configurations and descriptions. Optional Accessories: • Take3™ Micro-Volume Plate • Absorbance Test Plate • Fluorescence Test Plate • Luminescence Test Plate • Patented BioCell™ 1 cm quartz vessel • Product Qualification Package • Gen5™ Secure (for 21 CFR Part 11 Compliance) BioTek Instruments, Inc. Highland Park, P.O. Box 998 Winooski, Vermont 05404-0998, USA Phone: 802-655-4040 • Toll-Free: 888-451-5171 Outside the USA: 802-655-4740 www.biotek.com Gen5™ Data Analysis Software The Synergy™ 2 features a software platform with a modern and intuitive user interface. Typical Applications: • Nucleic acid quantification • Protein quantification • Enzyme kinetics • Biomarker quantification • ELISAs • Genetic analysis • Drug discovery • Cell proliferation • Cytotoxicity • Drug absorption and metabolism • Biologics drug discovery and development • Food safety • Biofuels research • Environmental monitoring AlphaScreen® /AlphaLISA® are registered trademarks of PerkinElmer. Specifications: General Detection mode: FL, TRF, FP, Lum., UV-Vis Abs., AlphaScreen® /AlphaLISA® Read method: End point, kinetic, spectral scanning, well area scanning Microplate types: 6 to 1536 wells (luminescence 1 to 384 wells) PCR plates Compatible with Take3™ Micro-Volume Plate Temperature control: 4-Zone™ incubation from 4 °C above ambient to 65 °C; ±0.2 °C at 37 °C Shaking: Yes Software: Gen5™ Data Analysis Software Automation: Compatible with BioStack™ and 3rd party automation AlphaScreen® /AlphaLISA® Light source: Tungsten halogen lamp Sensitivity: 100 amol of biotinylated-LCK-P peptide, 25 µL/well in 384-well plate Dynamic range: >6 decades Detection system: Ultra low noise PMT Absorbance Light source: SQ xenon flash lamp Wavelength selection: Monochromator Wavelength range: 200 – 999 nm, 1 nm increments Bandpass: 2.4 nm Dynamic range: 0 – 4.0 OD Resolution: 0.0001 OD Pathlength correction: Yes OD accuracy: <1 % at 2.0 OD <3% at 3.0 OD OD repeatability: <0.5% at 2.0 OD Reading speed: 96 wells: 11 seconds 384 wells: 22 seconds 1536 wells: 42 seconds Fluorescence Intensity Sensitivity (SF): Top: 1 pM (0.2 fmol/well 96-well plate; 0.1 fmol/well 384-well plate) Bottom: 5 pM (1 fmol/well 96-well plate; 0.5 fmol/well 384-well plate) Light source: Tungsten halogen High energy DPR xenon flash (optional) Wavelength selection: Deep blocking bandpass filters/dichroic mirrors Wavelength range: 300 – 700 nm with tungsten lamp (850 nm option) 200 – 700 nm with xenon lamp (850 nm option) Dynamic range: >6 decades Bandpass: Filter dependent Detection system: PMT Luminescence Sensitivity: 10 amol ATP (flash) Wavelength range: 300 – 700 nm Dynamic range: >6 decades detection system: Low noise PMT Fluorescence Polarization Light source: Tungsten halogen High energy DPR xenon flash (optional) Sensitivity: 3 mP at 1 nM fluorescein Wavelength selection: Deep blocking filters/dichroic mirrors Wavelength range: 400 – 700 nm (320 – 850 nm option) Time-Resolved Fluorescence Light source: High energy DPR xenon flash Sensitivity: Europium 60 fM (12 amol/well 96-well plate; 6 amol/well 384-well plate) Wavelength selection: Deep blocking filters/dichroic mirrors Wavelength range: 200 – 700 nm (850 nm option) Reagent Dispensers Number: 2 syringe pumps Dispense volume: 5 – 1000 µL in 1 µL increments Minimum prime volume: 1.1 mL, 100 µL with back flush Physical Characteristics Power: 100 – 240 Volts AC. 50/60 Hz Dimensions: 17”W x 17.5”D x 14.5”H (43.5 x 44.5 x 37.3 cm) Weight: 60 lbs (27 kg) Regulatory For In Vitro Diagnostic use. CE and TUV marked, RoHS compliant. Performance values represent the average observed factory test values. *Specifications subject to change. Rev. 10/21/14 IRAN BioTek , Viragene Akam Co. Tel : +9821 88611552-3 Fax : +9821 88044577