The Eukaryotic Cell Cycle ppt
- 2. • Eukaryotes have two major types of cell division: mitosis and meiosis • Mitosis is used to produce new body cells for growth and healing. • Meiosis is used to produce sex cells (eggs and sperm).
- 3. • Is an ordered series of events involving cell growth and cell division that produces two new daughter cells via mitosis. • The length of the cell cycle is highly variable even within the cells of an individual organism. In humans, the frequency of cell turnover ranges from a few hours in early embryonic development to an average of two to five days for epithelial cells, or to an entire human lifetime spent without dividing in specialized cells such as cortical neurons or cardiac muscle cells
- 4. • There is also variation in the time that a cell spends in each phase of the cell cycle. • The cell cycle has two major phases: interphase and the mitotic phase . • During interphase, the cell grows and DNA is replicated. • During the mitotic phase, the replicated DNA and cytoplasmic contents are separated and the cell divides.
- 6. • During interphase, the cell undergoes normal processes while also preparing for cell division. For a cell to move from interphase to the mitotic phase, many internal and external conditions must be met. The three stages of interphase are called G1, S, and G2. G1 PHASE • The first stage of interphase is called the G1 phase (first gap) . • during the G1 stage, the cell is quite active at the biochemical level.
- 7. • The cell is accumulating the building blocks of chromosomal DNA and the associated proteins as well as accumulating sufficient energy reserves to complete the task of replicating each chromosome in the nucleus. S PHASE (SYNTHESIS OF DNA) • In the S phase, DNA replication can proceed through the mechanisms that result in the formation of identical pairs of DNA molecules—sister chromatids— that are firmly attached to the centromeric region • The centrosome is also duplicated during the S phase.
- 8. • The two centrosomes will give rise to the mitotic spindle, the apparatus that regulated the movement of chromosomes during mitosis. • At the center of each animal cell, the centrosomes of animal cells are associated with a pair of rod-like objects, the centrioles, which are at right angles to each other.
- 9. • Centrioles help organize cell division. • Centrioles are not present in the centrosomes of other eukaryotic species, such as plants and most fungi.
- 10. G2 PHASE • The cell restore its energy and synthesizes proteins necessary for chromosome manipulation. • Some cell organelles are duplicated, and the cytoskeleton is dismantled to provide resources for the mitotic phase • The final preparations for the mitotic phase must be completed before the cell is able to enter the first stage of mitosis.
- 11. • The mitotic phase is a multistep process during which the duplicated chromosomes are aligned, separated, and moved to opposite poles of the cell, and then the cell is divided into two new identical daughter cells. • The first portion of the mitotic phase, mitosis, is composed of five stages, which accomplish nuclear division • The second portion of the mitotic phase, called cytokinesis, is the physical separation of the cytoplasmic components into two daughter cells.
- 13. • The nuclear envelope starts to dissociate into small vesicles, and the membranous organelles (such as the Golgi apparatus and endoplasmic reticulum), fragment and disperse toward the edges of the cell. • The nucleolus disappears. • The centrosomes begin to move to opposite poles of the cell. • Microtubules that will form the mitotic spindle extend between the centrosomes, pushing them farther apart as the microtubule fibers lengthen.
- 14. • The sister chromatids begin to coil more tightly with the aid of condensin proteins and become visible under a light microscope.
- 15. • The mitotic spindle continues to develop as more microtubules assemble and stretch across the length of the former nuclear area. • Chromosomes become more condensed and discrete. • Each sister chromatid develops a protein structure called a kinetochore in the centromeric region. • The proteins of the kinetochore attract and bind mitotic spindle microtubules.
- 16. • the spindle microtubules extend from the centrosomes, some of these microtubules come into contact with and firmly bind to the kinetochores . • a mitotic fiber attaches to a chromosome, the chromosome will be oriented until the kinetochores of sister chromatids face the opposite poles. • microtubules overlap each other midway between the two poles and contribute to cell elongation
- 18. • All the chromosomes are line up in a plane called the metaphase plate, or the equatorial plane, midway between the two poles of the cell. • The sister chromatids are still tightly attached to each other by cohesin proteins. At this time, the chromosomes are maximally condensed.
- 19. • The sister chromatids separate at the centromere. • Each chromatid, now called a chromosome, is pulled rapidly toward the centrosome to which its microtubule is attached. • The cell becomes visibly elongated (oval shaped) as the polar microtubules slide against each other at the metaphase plate where they overlap.
- 20. • The chromosomes reach the opposite poles and begin to decondense (unravel), relaxing into a chromatin configuration. • The mitotic spindles are depolymerized into tubulin monomers that will be used to assemble cytoskeletal components for each daughter cell. • Nuclear envelopes form around the chromosomes, and nucleosomes appear within the nuclear area.
- 21. • Cytokinesis, or “cell motion,” is the second main stage of the mitotic phase during which cell division is completed via the physical separation of the cytoplasmic components into two daughter cells. • The process of cytokinesis is quite different for eukaryotes that have cell walls, such as plant cells. • In cells such as animal cells that lack cell walls, cytokinesis follows the onset of anaphase. A contractile ring composed of actin filaments forms just inside the plasma membrane at the former metaphase plate
- 22. • The actin filaments pull the equator of the cell inward, forming a crack is called the cleavage furrow. The furrow deepens as the actin ring contracts, and eventually the membrane is cleaved in two. • In plant cells, a new cell wall must form between the daughter cells. • During interphase, the Golgi apparatus accumulates enzymes, structural proteins, and glucose molecules prior to breaking into vesicles and dispersing throughout the dividing cell
- 23. • During telophase, these Golgi vesicles are transported on microtubules to form a phragmoplast (a vesicular structure) at the metaphase plate. There, the vesicles fuse and coalesce from the center toward the cell walls; this structure is called a cell plate • As more vesicles fuse, the cell plate enlarges until it merges with the cell walls at the periphery of the cell. • Enzymes use the glucose that has accumulated between the membrane layers to build a new cell wall. The Golgi membranes become parts of the plasma membrane on either side of the new cell wall
- 25. • Not all cells adhere to the classic cell-cycle pattern in which a newly formed daughter cell immediately enters interphase, closely followed by the mitotic phase. • Cells in the G0 phase are not actively preparing to divide. • Some cells enter G0 temporarily until an external signal triggers the onset of G1 • Other cells that never or rarely divide, such as mature cardiac muscle and nerve cells, remain in G0 permanently.
- 26. 1. Cooper GM (2000). "Chapter 14: The Eukaryotic Cell Cycle". The cell: a molecular approach (2nd ed.). Washington, D.C: ASM Press. ISBN 978-0- 87893-106-4. 2. De Souza CP, Osmani SA (September 2007). "Mitosis, not just open or closed". Eukaryotic Cell. 6 (9): 1521–7. doi:10.1128/EC.00178-07. PMC 2043359. PMID 17660363. 3. Morgan DO (2007). The cell cycle : principles of control. London: New Science Press. ISBN 978-0-19-920610-0. OCLC 70173205. 4. Nelson DM, Ye X, Hall C, Santos H, Ma T, Kao GD, et al. (November 2002). "Coupling of DNA synthesis and histone synthesis in S phase independent of cyclin/cdk2 activity". Molecular and Cellular Biology. 22 (21): 7459–72. doi:10.1128/MCB.22.21.7459-7472.2002. PMC 135676. PMID 12370293. 5. Rubenstein I, Wick SM (2008). "Cell". World Book Online Reference Center. Archived from the original on 30 May 2011. Retrieved 10 July 2009.