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- 3. What is TLC?
• • Definition of Thin Layer Chromatography
• • Diagram of a TLC plate (insert image if
needed)
- 5. Components of TLC
• • Stationary phase: Silica gel or alumina
• • Mobile phase: Solvent
• • Sample: Compound to be separated
- 6. Materials Required
• • TLC plates
• • Developing chamber
• • Solvent system
• • Capillary tubes
• • UV lamp
- 7. Preparing the TLC Plate
• • Coating and drying
• • Activation by heating
• • Marking baseline
- 9. Developing the Plate
• • Place in solvent chamber
• • Solvent rises by capillary action
• • Mark solvent front
- 11. Calculating Rf Value
• Rf = Distance moved by compound / Distance
moved by solvent
• • Example: Rf = 2.5 cm / 5.0 cm = 0.5
- 13. TLC vs. Other Chromatography
• • Comparison with paper chromatography
• • Comparison with HPLC/Column
chromatography
- 14. Applications of TLC
• • Drug and pharmaceutical analysis
• • Purity checking
• • Reaction monitoring
• • Food and dye testing
- 16. Limitations of TLC
• • Semi-quantitative results
• • Limited resolution
• • Not for volatile samples
- 17. TLC in Modern Research
• • TLC-MS coupling
• • Preparative TLC
• • Forensic applications
- 19. Summary
• • TLC separates mixtures based on polarity
• • Quick and cost-effective
• • Useful in various fields
