This document discusses dental plaque biofilm and various toothbrushing methods and techniques. It begins by explaining how plaque biofilm leads to dental caries and periodontal diseases if not removed, and discusses the history of toothbrushes from ancient chewing sticks to modern designs. It then describes different manual toothbrushing techniques like the Bass method and Rolling method. Further sections cover powered toothbrushes and recommended toothbrushing time and frequency.