Use cases
- 1. Intereg Project Biomedical Informatics Ljiljana Majnarić Trtica III. Use Cases
- 2. 1) Early diagnosis of cancer Cancer is one of the widespread diseases leading to fatal death Among them, lung cancer and breast cancer accounts the most It has been found that if the disease is being diagnosed at an early stage, the survival rate of the patient could be improved However, in most of cases, the disease is being diagnosed at a later stage
- 3. 1) Early diagnosis of cancer There is an efficacious screening method (high resolution mammography), to be used for screening target female population on breast cancer The similar method does not exist for early detection of lung cancer Hence, the early diagnosis of lung cancer is the right topic to be an objective of predictive modelling, performed within DM and ML methods
- 4. Rajan JR, Prakash JJ. Early diagnosis of lung cancer using a mining tool. IJETICS, ISSN 2278-6856, 2013. The hospital reports of the patients suffering from lung cancer have been collected from various sources and integrated by the classification technique based on using ANN (Arteficial Neural Networks). The expert system, that was defined this way to support the diagnosis of lung cancer, was based on using information on risk factors and symptoms, rather than on images or biomarkers. Such approach is especially appropriate for use in less developed countries (India as an example).
- 5. 2) Predicting response to a drug therapy for cancer Cancer is a clinically and genomically heterogeneous disease Pharmaceutical companies apply DM to huge masses of genomic data to predict how a patient`s genetic makeup determines his/her response to a drug therapy
- 6. The Use Case: Daemen A et al. Modelling precision treatment of breast cancer. Genome Biology 2013; 14:R110 First-generation molecular profiles for human breast cancer have enabled identification of features that can predict therapeutic response However, little is known about how the various data types can best be combined to yield optimal predictors In this paper, collections of breast cancer cell lines was used to set cancer molecular pathobiology (omics- measurements) with biological therapeutic responses, in order to identify the most predictive molecular feature sets
- 7. The Use Case: Daemen A et al. Modelling precision treatment of breast cancer. Genome Biology 2013; 14:R110 Molecular profiling data sets included: profiles for DNA copy number, mRNA expression, transcriptome sequence, promoter methylation, protein abundance and mutation status Least squares-support vector machines and random forest algorithms were used to identify molecular features associated with responses
- 8. The Use Case: Daemen A et al. Modelling precision treatment of breast cancer. Genome Biology 2013; 14:R110 Results showed that matching patients to treatments based on transcriptional subtype will improve response rates, but that inclusion of additional features (from other profiling data types) may provide additional benefit A systems biology strategy has been suggested to guide clinical trials, that patient cohorts most likely to respond to new therapies may be more efficiently identified
- 9. The Use Case: Daemen A et al. Modelling precision treatment of breast cancer. Genome Biology 2013; 14:R110 (Fig.1) Fig.1. Molecular profilling procedures of the panel of breast cancer cell lines
- 10. 3) Using image diagnostics and data mining tools for early detection of other important chronic diseases The art of modern medicine is to identify subjects with early stages of chronic diseases, to enable preventive measures planning and timely treatment A lot of money has been spent in biomarkers development In addition to biochemical and molecular biology biomarkers, advanced imaging techniques have been emerged as a source of biomarkers
- 11. Dai Z, He Y. Disrupted structural and functional brain connectomes in mild cognitive impairment and Alzheimer`s disease. Neurosci Bull 2014; 30(2): 217– 232. Efforts of researchers, in this study, have been oriented towards using standard diagnostic imaging, to diagnose Alzheimer`s disease (AD), especially in its very early stages By using graph theory analyses, this study showed that AD disease and its early phases of development exhibit disrupted topological organization of large-scale brain networks and that this loss correlates well with the decline in cognitive functions By using combination of graph theory analyses and visual computer-based techniques, to enable user-friendly interfaces – very complex disease descriptions, based on using imaging diagnostic techniques, can become standard output reports on the desks of medical doctors and improve their insights into disease pathophysiology
- 12. 4) Using omics-techniques to improve understanding of pathophysiology of chronic diseases The enormous progress in biotechnology and computer-based techniques for massive data analysis, in the last decades, has provided clear benefits in medical practice by implementing genomics, proteomics and other OMICS-techniques in many sectors of health care By using these techniques, it is possible to diagnose many chronic diseases much earlier than before and to enable knowledge on molecular mechanisms and signaling pathways of many disorders - that has not been possible before The highest grade of these information integration has been achieved within systems biology and complex network analysis, holistic approaches aimed at revealing the function of biological regulatory networks
- 13. 4) Using omics-techniques to improve pathophysiology understanding The human microbiome is composed of microbes (mostly bacteria), that reside in the gut These microbes have tremendous potential to impact our physiology, both in health and in disease They contribute metabolic functions, protect against pathogens, educate the immune system, and, through these basic functions, affect directly or indirectly most of our physiologic functions
- 14. 4) Using omics-techniques to improve pathophysiology understanding The study of the human microbiome has been furthered by technological advancements for performing culture-independent analyses In most studies, the bacterial constituents of a microbial population are identified by sequencing of the 16S rRNA-encoding gene followed by comparison to known bacterial sequence databases Metagenomic analysis by sequencing all microbial DNA in a complex community has the additional advantage of assessing the genetic potential of the microbial population Other methodologies to analyze the microbial transcriptome, proteome, and metabolome provide additional information at successive levels of microbial physiology
- 15. The Use Case: Qin J, et al. A human microbial gene catalogue established by metagenomic sequencing. Nature 2010; 464(7285): 59- 65.(Fig. 2) Fig. 2. Bacterial species abundance differentiates patients with inflammatory bowel disease (ulcerative collitis & Crohn`s disease) and healthy individuals
- 16. 5) The vaccines development At the end of the 20th century, most of the vaccines that have been based on using traditional technologies (by killing and attenuating viruses causing the diseases) had been developed New technologies have been required to conquer the remaining pathogens, being refractory to vaccine development Remarkable progress was made during this period by the introduction of new technologies such as recombinant DNA and chemical conjugation of proteins to polysaccharides, as well as advances in the use of novel adjuvants Additionally, a powerful tool came from the ability to access the genomes of microorganisms This technological revolution allowed for the first time the capacity to move beyond the rules of Pasteur, using the computer to rationally design vaccines starting with information present in the genome, without the need to grow the specific microorganisms This new approach was denominated “reverse vaccinology”
- 17. The use case: Sette A, Rappuoli R. Reverse vaccinology: Developing vaccines in the era of genomics. Immunity 2010; 33(4): 530-41. (Fig. 3)
- 18. The use case: Sette A, Rappuoli R. Reverse vaccinology: Developing vaccines in the era of genomics. Immunity 2010; 33(4): 530-41. Fig. 3 description The first pathogen addressed by the reverse vaccinology approach was Meningococcus B (MenB) This pathogen was refractory to vaccine development because its capsular polysaccharide is identical to a human self- antigen, whereas the bacterial surface proteins are extremely variable Many attempts to develop a vaccine using the traditional technologies – have failed The project to sequence the MenB genome and to use the genomic information to develop a vaccine Gene sequences were analyzed, and over 600 potential antigens were tested for antigenicity Candidate sequences were expressed in Escherichia coli, and sera from immunized mice was obtained against each of them Analysis of the sera revealed more than 90 previously unknown surface located proteins (only 12 surface antigens were known, of them, only 4-5 showed bacterial activity) 29 out of 90 discovered antigens, were able to induce antibodies that could kill the bacteria in vitro in the presence of the complement In subsequent years, the antigens inducing the best and broadest bactericidal activity, were selected and inserted into prototype vaccines that were able to induce protective immunity against most of the MenB strains in mice After successful preclinical studies, the MenB vaccine entered the long path of vaccine development
- 19. 6)Prediction of health outcomes by using time series analysis A time serie is a serie of data points indexed in time order Most commonly, a time serie is a sequence taken at successive equally spaced points in time - thus, a sequence of discrete-time data There are two main goals of time series analysis: Recognition of patterns (trends) in some process evolution Prediction of outcomes
- 20. The use case: Sacchi L, Dagliati A, Segagni D, Leporati P, Chiovato L, Bellazzi R. Improving risk-stratification of Diabetes complications using temporal data mining. IEEE, 2015 Starting with the stratification of patients based on using temporal patterns of CSA (the index of temporal Medication Acquisition) Authors considered the clinical variables that characterize the patients’ clinical condition associated with the diagnosis of diabetes type 2 The stability in drug purchases turns out to be a marker for a group of patients who have an overall more complex clinical situation Chronic complications were shown to be more frequent in a group of patients more prone for medication changes over time
- 21. The use case: Sacchi L, Dagliati A, Segagni D, Leporati P, Chiovato L, Bellazzi R. Improving risk-stratification of Diabetes complications using temporal data mining. IEEE, 2015 (Fig. 4) Fig. 4. Predicting chronic complications in diabetic patients based on time series analysis of medication purchase