This study explored the acute effects of different glycemic index carbohydrates on endothelial function in 56 nondiabetic overweight and obese volunteers. Brachial artery flow-mediated dilation (FMD) was assessed after an overnight fast and 2 hours after consuming glucose, cornflakes, high-fiber cereal, or water. Post-prandial serum glucose levels were higher after glucose and cornflakes compared to fiber. FMD was reduced 2 hours after all meals but the reduction was statistically significant only for high glycemic index glucose and cornflakes. High glycemic index carbohydrate consumption significantly suppresses FMD compared to low glycemic index carbohydrates, suggesting it may enhance cardiovascular risk.