Worm sorting in Amplitude Modulated Microfluidic channels
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The document discusses a study by Parashar et al. (2011) that explores the adaptability of C. elegans locomotion within amplitude-modulated sinusoidal microchannels. Key findings demonstrate that wild-type C. elegans exhibit smooth movement in adaptable channel amplitude ranges, while encountering difficulties outside these ranges, as illustrated by varied velocity and stop duration metrics. The study utilizes high-resolution imaging and tracking programs to analyze locomotion patterns across different C. elegans strains.
![FIG. 7. (a) Snapshots of a L4-stage N2 C. elegans whose body positions and centroid are tracked by a worm tracking program. The tracks show the relative levels of
difficulty faced by the worm in the different sections of the channel [(i): lower cut-off region, (ii): adaptable region, and (iii): higher cut-off region (see Ref. 29)] (b)
Representative tracks of the body centroid for C. elegans (N2, lev-8 and unc-38) along the modulated sinusoidal channel.
Parashar, A., Lycke, R., Carr, J. A., & Pandey, S. (2011). Amplitude-modulated sinusoidal microchannels for observing adaptability in C. elegans locomotion. Biomicrofluidics, 5(2), 024112. https://doi.org/10.1063/1.3604391
https://aip.scitation.org/doi/10.1063/1.3604391](https://image.slidesharecdn.com/amplitude-230218152513-97e05423/85/Worm-sorting-in-Amplitude-Modulated-Microfluidic-channels-7-320.jpg)
Worm sorting in Amplitude Modulated Microfluidic channels
- 1. Parashar, A., Lycke, R., Carr, J. A., & Pandey, S. (2011). Amplitude-modulated sinusoidal microchannels for observing adaptability in C. elegans locomotion. Biomicrofluidics, 5(2), 024112. https://doi.org/10.1063/1.3604391 https://aip.scitation.org/doi/10.1063/1.3604391 FIG. 1. (a) Experimental setup comprising a microfluidic chip with sinusoidal channels, high-resolution microscope, and worm tracking program. (b) Magnified images of the two (i.e., with increasing and decreasing amplitude) modulated sinusoidal channels with vertical markers.
- 2. FIG. 2. Wild-type C. elegans (encircled) crawling in different sections of a modulated sinusoidal channel. The worm shows relatively smooth movement in the adaptable range of channel amplitudes (b-e). In sections beyond this adaptable range (a, f), the worm is unable to move forward. Parashar, A., Lycke, R., Carr, J. A., & Pandey, S. (2011). Amplitude-modulated sinusoidal microchannels for observing adaptability in C. elegans locomotion. Biomicrofluidics, 5(2), 024112. https://doi.org/10.1063/1.3604391 https://aip.scitation.org/doi/10.1063/1.3604391
- 3. FIG. 3. (a) Average forward velocity versus channel amplitude is plotted for the three L4-stage C. elegans strains (N2, lev-8 and unc-38). (b) Average forward velocity and ratio of amplitude to wavelength (A;) for the three C. elegans strains on 2.5% agarose plates are shown. Parashar, A., Lycke, R., Carr, J. A., & Pandey, S. (2011). Amplitude-modulated sinusoidal microchannels for observing adaptability in C. elegans locomotion. Biomicrofluidics, 5(2), 024112. https://doi.org/10.1063/1.3604391 https://aip.scitation.org/doi/10.1063/1.3604391
- 4. FIG. 4. Average number (a) and duration (b) of stops versus channel amplitude are plotted for the three C. elegans strains (N2, lev-8 and unc-38). Parashar, A., Lycke, R., Carr, J. A., & Pandey, S. (2011). Amplitude-modulated sinusoidal microchannels for observing adaptability in C. elegans locomotion. Biomicrofluidics, 5(2), 024112. https://doi.org/10.1063/1.3604391 https://aip.scitation.org/doi/10.1063/1.3604391
- 5. FIG. 5. (a) Illustration of the range of contact angle for a L4-stage N2 C. elegans in two sections of the modulated sinusoidal channel. (b) Range of contact angle versus channel amplitude is plotted for the L4-stage C. elegans (N2, lev-8 and unc-38). Parashar, A., Lycke, R., Carr, J. A., & Pandey, S. (2011). Amplitude-modulated sinusoidal microchannels for observing adaptability in C. elegans locomotion. Biomicrofluidics, 5(2), 024112. https://doi.org/10.1063/1.3604391 https://aip.scitation.org/doi/10.1063/1.3604391
- 6. FIG. 6. The lower and upper cut-off regions in the modulated sinusoidal channels are shown for the N2, lev-8 and unc-38 C. elegans. Parashar, A., Lycke, R., Carr, J. A., & Pandey, S. (2011). Amplitude-modulated sinusoidal microchannels for observing adaptability in C. elegans locomotion. Biomicrofluidics, 5(2), 024112. https://doi.org/10.1063/1.3604391 https://aip.scitation.org/doi/10.1063/1.3604391
- 7. FIG. 7. (a) Snapshots of a L4-stage N2 C. elegans whose body positions and centroid are tracked by a worm tracking program. The tracks show the relative levels of difficulty faced by the worm in the different sections of the channel [(i): lower cut-off region, (ii): adaptable region, and (iii): higher cut-off region (see Ref. 29)] (b) Representative tracks of the body centroid for C. elegans (N2, lev-8 and unc-38) along the modulated sinusoidal channel. Parashar, A., Lycke, R., Carr, J. A., & Pandey, S. (2011). Amplitude-modulated sinusoidal microchannels for observing adaptability in C. elegans locomotion. Biomicrofluidics, 5(2), 024112. https://doi.org/10.1063/1.3604391 https://aip.scitation.org/doi/10.1063/1.3604391