Histo Slides with identifecation points (1) (1).pdf
- 3. TS of Bone
- 4. T.S BONE 1.TS section of haversian system with concentric lamellae around haversian canal is seen. 2.Lacunae containing osteocytes present. 3.Circumferential lamellae and interstitial lamellae seen. 4.Periosteum present
- 5. LS of Bone
- 6. L.S OF BONE 1.L.S of haversian system with central haversian canals. 2.Volkmanns canal - interconnecting haversian canals seen. 3.Lacunae containing osteocytes seen.
- 8. HYALINE CARTILAGE Identification Points: • Dense Irregular CT (perichondrium) • Chondrocytes within lacunae • Chondrocytes in the form of isogenous groups • Naked eye examination: Bluish white translucent appearance
- 10. ELASTIC CARTILAGE Identification Points: • Perichondrium is present • Elastic fibres in matrix • Basophilic ground substance around lacunae • Chondrocytes in lacunae in the form of isogenous groups
- 12. FIBRO CARTILAGE Identification Points: • No perichondrium • Chondrocytes within lacunae, arranged in rows • Collagen fibres arranged parallel
- 13. Skeletal Muscle
- 14. SKELETAL MUSCLES Identification Points: • Characteristic striated appearance • Peripheral nuclei • Nucleus ovoid in shape
- 15. Cardiac Muscle
- 16. CARDIAC MUSCLE Identification Points: • Branched • Striated • Intercalated discs
- 17. Large Vein
- 18. Large vein 1.Three layers tunica media tunica media, tunic adventitia is present. 2 .tunica externa thickest with longitudinal fibers present.
- 19. Large Artery
- 20. LARGE /ELASTIC ARTERY Identification Points: • No distinct outer and inner elastic membranes • Tunica adventitia: merges with CT, contains vasa vasorum • Tunica media: elastic fibres are present in a large amount • Tunica intima: Simple squamous endothelium, subendothelial CT
- 22. MEDIUM SIZE/MUSCULAR ARTERY Identification Points: • Tunica intima: darkly stained • Tunica media: smooth muscle fibres arranged in circular patterns • Tunica adventitia: collagen (lightly stained); elastic (darkly stained)
- 23. Lymph Node
- 24. LYMPH NODE Identification Points: • Primary follicles/nodules without germinal centre • Secondary follicles/nodules with germinal centre • Irregular anastomosing ‘medullary cords’ • Subcapsular sinuses
- 25. Palatine Tonsil
- 26. TONSIL Identification Points: • Stratified squamous non keratinized epithelium • Crypt • Trabeculae • Lymphatic nodules
- 27. Thymus
- 28. THYMUS Identification Points: • Medulla is lightly stained • Hassall’s corpuscles in medulla • Epithelioreticular cells; stellate shaped
- 29. Spleen
- 30. SPLEEN Identification Points: • Red pulp: splenic cord and venous sinuses • White pulp: contains lymphatic nodules and germinal centre • Splenic sinuses • No differentiation into cortex and medulla
- 32. MIXED SALIVARY GLAND : 1.Serous acini with biphasic staining of pyramid cells. 2.Mucous acini with empty looking larger and flat basal nucleus and lumen are seen. 3.Serous demilunes capping mucous acini seen.
- 34. MUCOUS SALIVARY GRAND 1.empty looking larger mucous acini with flat basal nuclei and large lumen. 2.interlobular ducts present
- 36. SEROUS SLIVARY GLAND 1. Serous acini with biphasic staining of pyramidal cells. 2. Striated and interlobular ducts seen.
- 38. DIGESTIVE SYSTEM
- 39. Pancreas
- 40. PANCREAS 1.serous acini with biphasic stain and centroacinar cells 2.islets of Langerhans seen
- 41. Liver
- 42. LIVER 1.hexagonal-shaped hepatic lobules with central vein and radiating hepatocytes with sinusoids in between seen 2.portal traid—containing branch of portal vein, hepatic artery and bile ductule present at the corners of lobules seen
- 43. Gall Bladder
- 44. GALLBLADDER 1.mucosa thrown into folds lined by simple tall columnar epithelium with brush border 2.fibromuscular wall seen
- 45. Tongue
- 46. Tongue 1.surface lined by stratified squamous non keratinized epithelium 2.surface shows projections called papillae 3.three types of papillae are-circumvallate/fungiform/filiform 4.circumvallate and fungiform papillae contain taste buds 5.cut sections of muscles and seromucous glands seen
- 47. Oesophagus
- 48. ESOPHAGUS 1.mucosa lined by non keratinized stratified squamous epithelium 2.mucus secreting esophageal glands in the submucosal layer
- 49. Stomach Fundus
- 50. STOMACH:FUNDUS AND BODY 1.mucosal lining is simple columnar epithelium 2.shallow gastric pits present 3. Lamina propria filled with deep straight tubular glands with parietal and chief cells
- 51. Stomach Pylorus
- 52. STOMACH:PYLORUS 1.mucosal lining is simple columnar epithelium 2.deep gastric pits 3.lamina propria filled with coiled tubular glands
- 53. Duodenum
- 54. DUODENUM 1.mucosal villi,lined by columnar epithelium with microvilli(brush border)and goblet cells 2.submucosal brunner's glands present
- 55. Jejunum
- 56. JEJUNUM 1.mucosal villi,lined by columnar epithelium with microvilli(brush border) and lots of globet cells 2.intestinal glands(crypts of lieberkiihn)present 3.no submucosal glands or Peyer's patch
- 57. Iieum
- 58. ILEUM 1.mucosal villi,lined by columnar epithelium with microvilli (brush border)and lots of globet cells 2.peyer's patch in mucosal layer extending to submucosa
- 59. Appendix
- 60. APPENDIX 1.lining epithelium is tall columnar with lots of globet cells 2.lamina propria filled with lymphoid follicles and few crypts of lieberkiihn 3.no vill,no taenia coil
- 61. Large Intestine
- 62. LARGE INTESTINE 1. lining epithelium is tall columnar with lots of globet cells 2.lamina propria filled with crypts of lieberkiihn 3.taenia coil in the muscularis externa
- 64. Lung
- 65. LUNG 1. Cut sections of intrapulmonary bronchi and bronchioles are seen 2.Alveoli lined by Simple Squamous Epithelium
- 66. Trachea
- 67. TRACHEA- 1.Pseudostratified ciliated columnar epithelium with goblet cells lines the mucosa 2.Serous and mucus glands in the submucosa seen. 3.Thick layer of Hyaline Cartilage present.
- 68. URINARY SYSTEM
- 69. Kidney
- 70. Kidney 1.Outer Cortex-Renal corpuscles and sections of proximal and distal convoluted tubules seen 2.Inner Medulla-Sections of collecting ducts and loop of Henle seen
- 71. Ureter
- 72. Ureter 1.Mucosal folds lined by transitional epithelium 2.Muscular tube-inner longitudinal,outer circular layer of smooth muscles.
- 73. Urinary Bladder
- 74. Urinary Bladder 1.Mucosa lined by transitional epithelium. 2.Thick muscular wall made of three ill-defined layers. 3.Thick lamina propria.
- 76. Testis
- 77. Testis 1.Sections of Seminiferous tubules lined by spermatogonia,different stages of spermatocytes and Sertoli cells. 2.Interstitial cells of Leydig in between the tubules seen.
- 78. Epididymis
- 79. Epididymis 1.Sections of tubule lined by Pseudostratified columnar epithelium with stereocilia. 2.Lumen filled with sperms. 3.Each tube surrounded by smooth muscle cells.
- 80. Prostat e
- 81. Prostate 1.Prostatic glands (acini or follicles) lined by simple columnar epithelium. 2.Mucosa thrown into folds,lumen contains Corpora amylacea. 3.Acini separated by thick fibromuscular stroma.
- 82. Vas Deferens
- 83. Vas deferens 1.Mucosa lined by Simple Columnar cells (towards the distal end,it is lined by Pseudostratified Columnar epithelium). 2.Thick muscular tube.
- 85. Uterus
- 86. Uterus: Proliferative 1.Inner endometrium-lined by simple columnar cells Stroma contains tubular Uterine glands and spiral arteries. 2.Middle thick muscular layer-Myometrium 3.Outer Perimetrium
- 87. Uterus
- 88. Uterus: Secretory 1.Inner endometrium-lined by simple columnar cells Stroma contains tubular Uterine glands and spiral arteries. 2.Middle thick muscular layer-Myometrium 3.Outer Perimetrium
- 89. Fallopian Tube
- 90. Fallopian tube 1.Lining epithelium-simple columnar cells with cilia. Mucosa thrown into folds filling the lumen. 2.Muscular tube.
- 91. Ovary
- 92. Ovary 1.Outer Cortex-shows ovarian follicles in different stages of development- Primordial,Primary,Secondary and Graffian follicle. 2.Inner Medulla-contains connective tissue with blood vessels.
- 93. Placenta
- 94. Placenta 1.Sections of Chorionic villi seen. 2.Villi with embryonic connective tissue core containing fetal capillaries and surrounded by outer Syncytiotrophoblasts and inner Cytotrophoblasts.
- 95. Umbilical Cord
- 96. Umbilical Cord 1.Two muscular arteries and one vein seen. 2.Wharton's jelly (embryonic mesenchyme) surrounding the vessels with outer amniotic covering.
- 97. ENDOCRINE GLANDS
- 98. Thyroid Gland
- 99. Thyroid gland 1.Sections of follicles of various sizes filled with colloidal,lined by cuboidal cells. 2.Parafollicular cells present. 3.Highly vascular tissue-plenty of capillaries seen.
- 100. Pitutary Gland
- 101. Pituitary gland 1.Adenohypophysis with Chromophobes and Chromophills arranged in groups. 2.Neurohypophysis with nerve fibres and pituicytes.
- 102. Suprarenal Gland
- 103. Suprarenal gland 1.Cortex and medulla differentiated. 2.Cortex with outer Zona glomerulosa,middle Zona fasciculata and inner Zona reticularis. 3.Medulla with polyhedral Chromaffin cells in groups.
- 104. NERVOUS TISSUE
- 105. Spinal Cord
- 106. SPINAL CORD Identification Points: • Central canal • Butterfly shaped grey matter • Dorsal median sulcus and ventral median fissure (depending on the portion under examination)
- 107. Cerebrum
- 108. CEREBRUM Identification points: • Pyramidal and non pyramidal cells • Arranged in the form of layers (mention the names) – properly visible only by ‘Nissl staining method’
- 109. Cerebellum
- 110. CEREBELLUM Identification Points: From outwards to inwards: • Molecular layer with fan shaped dendrites, stellate cells and basket cells (but cell population is very low) • Flask shaped Perkinge cells • Granular layer (deeply basophilic appearance of closely packed nuclei)
- 111. Sympathetic Ganglion
- 112. Sympathetic Ganglion 1.Small multipolar neurons with eccentric nucleus surrounded by satellite cells. 2.Scattered between the nerve fibres. 3.Thin connective tissue capsule.
- 113. Spinal Ganglion
- 114. Spinal Ganglion/Dorsal root Ganglion 1.Large pseudounipolar neurons with central nucleus surrounded by satellite cells. 2 Arranged in groups between bundles of nerve fibres. 3.Thick connective tissue capsule.
- 115. SPECIAL SENSES
- 116. Cornea
- 117. Cornea 1.Five layered Structure. 2.Outer Stratified squamous epithelium-non keratinized. 3.Middle thick layer of Substantia propria.
- 118. Retina
- 119. Retina 1.Ten layered structure. 2.Outer most pigment cell layer,nuclear and ganglion cell layer are prominent. 3.Rods and cones are present.
- 120. Thick Skin
- 121. THICK SKIN Identification Points: • Stratified squamous keratinized epithelium • No hair roots and follicles • Pacinian corpuscles
- 122. Thin Skin
- 123. THIN SKIN Identification Points: • Stratified squamous keratinized epithelium • Dense irregular CT beneath • Hair follicles and roots present • Sebaceous glands • Sweat glands