compPcompA are two component systems for regulating response and ge.pdf
- 1. compP/compA are two component systems for regulating response and gene expression in response to cell density. Genetic competence in Bacillus Subtilis is that physiological state which enables bacteria to bind to attain high molecular weight exogenous DNA. In Bacillus subtilis, competence develops postexponentially and only in particular media. One of the mechanism is Natural competence, and it is subjected to three regulatory modes: growth stage specific, nutritionally responsive, and cell type specific. Virulence proteases expression by ComP/ComA : When an ComP/ComA complex shows impact on neamtoxic activites in B. subtilis. We can see change in protein hydrolysis and mortality rare of nematode. When an strain ComP mutant and comP, we can see in comP protease activity has been lost. In case of Compotent ComP mutant these functions are normal. This proves the there is a strong propational relationship between ComP/ComA complex and protein hydrolysis for virulence . comP, comA, mutations were known to severely impair competence. Each mutation also causes a reduction in srfA expression. by expressing srfA independently of ComP, ComA, and SpoOK and determining whether this will permit competence development. The results showed that the PspacsrfA construction provides the means to address this issue, as expression of Pspac-srfA does not require ComP, ComA. Solution compP/compA are two component systems for regulating response and gene expression in response to cell density. Genetic competence in Bacillus Subtilis is that physiological state which enables bacteria to bind to attain high molecular weight exogenous DNA. In Bacillus subtilis, competence develops postexponentially and only in particular media. One of the mechanism is Natural competence, and it is subjected to three regulatory modes: growth stage specific, nutritionally responsive, and cell type specific. Virulence proteases expression by ComP/ComA : When an ComP/ComA complex shows impact on neamtoxic activites in B. subtilis. We can see change in protein hydrolysis and mortality rare of nematode. When an strain ComP mutant and comP, we can see in comP protease activity has been lost. In case of Compotent ComP mutant these functions are normal. This proves the there is a strong propational relationship between ComP/ComA complex and protein hydrolysis for virulence . comP, comA, mutations were known to severely impair competence. Each mutation also causes
- 2. a reduction in srfA expression. by expressing srfA independently of ComP, ComA, and SpoOK and determining whether this will permit competence development. The results showed that the PspacsrfA construction provides the means to address this issue, as expression of Pspac-srfA does not require ComP, ComA.