CAPFITOGEN tools. Facilitated spatial and ecogeographical germplasm analysis for efficient PGR conservation and utilization
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The document outlines the development of tools for spatial and ecogeographical analysis to enhance the conservation and utilization of plant genetic resources (PGR) in Latin America. Funded initiatives included training and capacity building programs, focusing on ex situ and in situ conservation methods while generating ecogeographical characterizations and diversity maps for better germplasm collection strategies. The project aimed to strengthen national plant genetic resource capacities through various workshops and the release of tools in multiple languages.
CAPFITOGEN tools. Facilitated spatial and ecogeographical germplasm analysis for efficient PGR conservation and utilization
- 1. Tools Facilitated spatial and ecogeographical germplasm analysis for efficient PGR conservation and utilization Mauricio Parra Quijano (ITPGRFA - Programme Coordinator) Francisco López (ITPGRFA) Elena Torres (UPM-Spain) José Iriondo (URJC-Spain)
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- 3. Funded by: And with technical support of:
- 4. Development Transfer Training Building Capacity National Programs and institutions from Latin America
- 5. Idea + methodology development Theoretical and practical scientific validation Publication (peer review) Data preparation R script development Java / tomcat interface Tool + documentation Assistance CAPFITOGEN tool release / distribution GIS and ecogeographic approaches on PGR
- 8. 40° 20’ 33.4’’ N 03° 11’ 52.1’’ W Or paper maps… Gazeteers… Georeferencing software Old records… Evaluate the quality of your collecting sites coordinates and locality description
- 9. Quality assessment on the georeferencing of the collecting sites included in passport data
- 11. Based on: Parra-Quijano et al., 2012. Gen Res Crop Evol 59(2):205-217
- 12. Obtaining Ecogeographic Land Characterization (ELC) maps for a single species or group of related (specific maps) or unrelated species (generalist maps)
- 13. Ecogeographic germplasm (collecting sites) characterizations Up to 105 ecogeographic variables Ecogeographic Characterization matrix Georeferenced Passport data GIS Elevation Annual mean temp Organic carbon on soil pH soil …. …. Y X
- 14. Perform ecogeographic characterizations of the georeferenced collecting sites
- 15. • Is your collection representative of the species’ environmental adaptive range? Check the ecogeographical representativeness of your ex situ collection GAPS! REPRESENTED
- 16. Evaluation of the ecogeographical representativeness of the germplasm collections
- 17. • Ocurrence of the highest levels of diversity in terms of average genotypic, phenotypic or ecogeographic distances among local Identify where is located the diversity hotspots using phenotypic, genotypic or ecogeographic data Maize study Andean region Ecuador Inspired on Van Zonneveld et al., 2012. PLoS ONE 7(1): e29845
- 18. Obtaining phenotipic, genotypic or ecogeographic “diversity” maps
- 19. Select a subset of accessions which represents the species’ ecogeographical range in your collection A B A A A A B BB B C BA A B C A A A AB B B B CBA C A A A A A A A A CAB A B CEcogeographic Core Collection Based on Parra Quijano et al., 2011. Crop Science 51:656-666
- 20. Generation of ecogeographic core collections based on ELC maps
- 21. Creating subsets of accessions with high probability to contain interesting traits Annual precipitation Clay content Slope 40 4 10% selection intensity FIGS subset (by filtering method) Based on FIGS approach by Mackay,M., Street, K., Bari, A., Endresen, D. and other researchers Waterlogging tolerance
- 22. Generation of subsets of breeding interest traits based on ecogeographic conditions of the collecting sites (Focused Identification Germplasm Strategy – filtering method)
- 23. CAPFITOGEN tools offer: • Avoiding collecting in sites where other have been collecting in past expeditions • Collecting germplasm in under or non-represented environments • Collecting germplasm in sites with high probably of traits of interest occurrence
- 24. GEOQUAL + ELCmapas + Representa + FIGS_R
- 27. 105 ecogeographical variables to select With data available for more than 160 countries (national work frames) Two sub-continental work frames (South and Meso- America) One continent (Europe) and a world-wide work frame 1x1, 5x5, 10x10 and 20x20 km resolutions Free access, free distribution
- 28. Programme to Strengthen National Plant Genetic Resource Capacities in Latin America Phase 1: 2012-2014 Seven tools focused on ex situ conservation User guides Three languages (Spanish, English, Portuguese) Two regional (Latin American) workshops Four national workshops (Cuba, Spain, Honduras, Uruguay) About 150 trained technicians
- 29. Programme to Strengthen National Plant Genetic Resource Capacities in Latin America Phase 2: 2014-2016 Three new tools focused on in situ conservation User’s networks E-Learning methods Tools on server (internet connection required, no installation) One final regional (Latin America + Spain + Portugal) workshop Four national workshops
- 30. Madrid, Spain, Nov 2013 Bogotá, Colombia, March 2013 Colonia, Uruguay, April 2014 Comayagua, Honduras, Nov 2013 Sancti Spitirus, Cuba, Sept 2013 Florianópolis, Brazil, May 2014 Birmingham, UK, Feb 2014