PHP Data Objects (PDO) is a PHP extension that provides a lightweight and consistent interface for accessing databases. It works as a connector between PHP applications and supported databases like MySQL, PostgreSQL, SQLite, and others. PDO supports prepared statements to protect against SQL injection and allows data to be fetched as associative arrays, numeric arrays, objects, or custom class objects. In TYPO3 Flow, PDO is used through the PDOConnection class to connect to and query databases configured in Settings.yaml.