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fruitbreedomics

This document summarizes a technical session on pyramiding scab and mildew resistance genes in apple breeding at Agroscope. The objective is to cross parent lines with different resistance genes to combine two or more genes against the same pathogen. Two crosses were made between parent lines containing different resistance genes for apple scab (Rvi6 and Rvi2) and powdery mildew (Pl2). SNP markers were used to analyze the crosses and determine which resistance genes were passed to the progeny. The document demonstrates using Excel to interpret the results of the marker analysis and determine which resistance genes were combined in the progeny for future disease resistance.

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FruitBreedomics – Technical Session Pyramiding scab and mildew resistance in the Agroscope breeding program I. Baumgartner (Agroscope) San Giuseppe di Comacchio (Italy), June 19th 2015
Overview • Objective: pyramiding resistance genes  two or more genes against the same pathogen • Crosses: parent selection (traits? pedigree?) • SNP markers • Polymorphism in parents • Interpretaion of results: demonstration in Excel
Mother (Resistances) Father (Resistances) Flowers Fruits Seeds ACW 11303 (Rvi6=Vf) ACW 18522 (Rvi6=Vf, Rvi2=Vh2) 2246 352 2227 ACW 13652 (Rvi6=Vf, Pl2) ACW 11567 (Rvi2=Vh2) 1370 323 2793 Progeny 1 Progeny 2 Pilot studies crosses at Agroscope
• Phenotypic symptoms (single resistance genes)? • Pedigree? Be sure about the resistances
LG1 Rvi6 resistance to apple scab Scab and mildew resistances located on different chromosomes LG2 LG3 LG4 LG5 LG6 LG7 LG8 LG9 LG10 LG11 LG12 LG13 LG14 LG15 LG16 LG17 Rvi2 resistance to apple scab Pl2 resistance to powdery mildew Apple genetic linkage map (17 chromosomes)
Trait / Locus Marker Scab resistance Rvi6=Vf (LG1) Rvi6_42M10SP6_Y124 (SNP) Scab resistance Rvi2=Vh2 (LG2) Rvi2_region53_M417 (SNP) Mildew resistance Pl2 (LG11) Pl2_3_Y211 (SNP) Fruit texture Md-PG1 (LG10) PG_FEM_LC_19 (SNP) Shelf life Md-ACO1 (LG10) ACO_FEM_cg_4 (SNP) Shelf life Md-ACS1 (LG15) ACS_FEM_cg_5 (SNP) Crispness (LG16) Crispness_SNP1 (ss475881704; SNP1 from RosBREED) Acidity (LG16) Acidity_SNP2 (ss475876558; SNP2 from RosBREED) Markers used
Literature on SNPs for scab and mildew resistance
Code SNP M A/C R A/G W A/T S C/G Y C/T K G/T H A/C/T V A/C/G D A/G/T B C/G/T Jänsch et al. 2015
Polymorphism in parents Cross 1206 Rvi6_42M10SP6_Y124 Rvi2_region53_M417 Pl2_3_Y211 ACW13652 CT CC CT ACW11567 CC CA CC T = Rvi6 resisance A = Rvi2 resistance T = Pl2 resistance Cross 1205 Rvi6_42M10SP6_Y124 Rvi2_region53_M417 ACW11303 CT CC ACW18522 CT CA T = Vf resisance A = Rvi2 resistance
Demonstration in Excel
Thank you for your attention powdery mildew Agroscope good food, healthy environment

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04 baumgartner

  • 1. FruitBreedomics – Technical Session Pyramiding scab and mildew resistance in the Agroscope breeding program I. Baumgartner (Agroscope) San Giuseppe di Comacchio (Italy), June 19th 2015
  • 2. Overview • Objective: pyramiding resistance genes  two or more genes against the same pathogen • Crosses: parent selection (traits? pedigree?) • SNP markers • Polymorphism in parents • Interpretaion of results: demonstration in Excel
  • 3. Mother (Resistances) Father (Resistances) Flowers Fruits Seeds ACW 11303 (Rvi6=Vf) ACW 18522 (Rvi6=Vf, Rvi2=Vh2) 2246 352 2227 ACW 13652 (Rvi6=Vf, Pl2) ACW 11567 (Rvi2=Vh2) 1370 323 2793 Progeny 1 Progeny 2 Pilot studies crosses at Agroscope
  • 4. • Phenotypic symptoms (single resistance genes)? • Pedigree? Be sure about the resistances
  • 5. LG1 Rvi6 resistance to apple scab Scab and mildew resistances located on different chromosomes LG2 LG3 LG4 LG5 LG6 LG7 LG8 LG9 LG10 LG11 LG12 LG13 LG14 LG15 LG16 LG17 Rvi2 resistance to apple scab Pl2 resistance to powdery mildew Apple genetic linkage map (17 chromosomes)
  • 6. Trait / Locus Marker Scab resistance Rvi6=Vf (LG1) Rvi6_42M10SP6_Y124 (SNP) Scab resistance Rvi2=Vh2 (LG2) Rvi2_region53_M417 (SNP) Mildew resistance Pl2 (LG11) Pl2_3_Y211 (SNP) Fruit texture Md-PG1 (LG10) PG_FEM_LC_19 (SNP) Shelf life Md-ACO1 (LG10) ACO_FEM_cg_4 (SNP) Shelf life Md-ACS1 (LG15) ACS_FEM_cg_5 (SNP) Crispness (LG16) Crispness_SNP1 (ss475881704; SNP1 from RosBREED) Acidity (LG16) Acidity_SNP2 (ss475876558; SNP2 from RosBREED) Markers used
  • 7. Literature on SNPs for scab and mildew resistance
  • 8. Code SNP M A/C R A/G W A/T S C/G Y C/T K G/T H A/C/T V A/C/G D A/G/T B C/G/T Jänsch et al. 2015
  • 9. Polymorphism in parents Cross 1206 Rvi6_42M10SP6_Y124 Rvi2_region53_M417 Pl2_3_Y211 ACW13652 CT CC CT ACW11567 CC CA CC T = Rvi6 resisance A = Rvi2 resistance T = Pl2 resistance Cross 1205 Rvi6_42M10SP6_Y124 Rvi2_region53_M417 ACW11303 CT CC ACW18522 CT CA T = Vf resisance A = Rvi2 resistance
  • 11. Thank you for your attention powdery mildew Agroscope good food, healthy environment