This document discusses biological species and speciation. It defines a biological species as members of a population that can breed and produce fertile offspring. It describes reproductive isolating mechanisms that prevent interbreeding between species, including prezygotic mechanisms like behavioral, temporal, ecological, mechanical, and gametic isolation and postzygotic mechanisms like zygotic mortality, hybrid inviability, and hybrid infertility. Speciation occurs through allopatric speciation by physical separation of populations or sympatric speciation within a single population through disruptive selection on extreme traits.