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1-) Why did we have an unstained sample- a CD4-PE only sample- and a.pdf

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The document discusses the necessity of including various samples in flow cytometry, such as unstained, CD4-PE only, and CD8-FITC only samples, alongside double-stained samples. It highlights the application of basic immunological principles in the staining process of cells through flow cytometry. Additionally, it explores the role of flow cytometry in biological research.

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1.) Why did we have an unstained sample, a CD4-PE only sample, and a CD8-FITC only sample in addition to the same stained with CD4-PE and CD8-FITC? How does flow cytometry utilize basic principles of immunology when staining cells? How can flow cytomery be utilized in biological studies?

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1-) Why did we have an unstained sample- a CD4-PE only sample- and a.pdf

  • 1. 1.) Why did we have an unstained sample, a CD4-PE only sample, and a CD8-FITC only sample in addition to the same stained with CD4-PE and CD8-FITC? How does flow cytometry utilize basic principles of immunology when staining cells? How can flow cytomery be utilized in biological studies?