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100422-intro,setup
- 1. 2010/04/22 kaneko.satoko(at)ocha.ac.jp
- 2. 10
- 5. 1953 DNA 1966 ( ) 1972 1975‐7 1985 PCR 1986 1993 1995 DNA 1997 E.coli ( ) (4.6Mb) 2000 2003 (3.3Gb)
- 6. 1972 1975‐7 (Sanger Maxam‐Gilbert ) 1985 PCR (6kb = 6000bp/day) 1986 DNA 1993 2000 (600kb = 600,000bp/day) 2007 Microarray like sequence (600Mb = 600,000,000bp/day) 2010 ? (100Gb = 100,000,000,000bp/hour) (3.3Gb) 4
- 7. 1971 MEDLINE 1980 DNA EMBL(European Molecular Biology Laboratory) 1982 DNA GenBank 1985 FASTA 1986 Swiss‐Prot 1988 NCBI(Na[onal Center for Biotechnology Informa[on) Human Genome Ini[a[ve Bioinforma[cs CLUSTAL 1990 BLAST 1991 World Wide Web 2000 Ensembl , 2001 UCSC
- 8. 1991 Linda B. Buck and Richard Axel (The Nobel Prize in Physiology or Medicine for 2004) G primer PCR (a) (b) (c) (18 loci) (a) (b) (c) (from & ) 1992 1998
- 9. 1999 Richard Axel John Carlson 2007 Drosophila 12 Genomes Consor[um Drosophila melanogaster( ) 11 12 (812 loci) (from )
- 10. (1) ATGC 35 1946 (DNA ) 8/20=40% 16/19=84%
- 11. DNA (2): ‐ (omics)‐
- 12. 1958 Francis Crick (DNA) DNA RNA RNA replica[on transcrip[on transla[on ( ) ( ) ( ) reverse transcrip[on ( ) splicing ( ) RNA (non‐coding RNA)
- 13. (2): exon1 exon2 exon3 exon4 exon5 exon6 5' 3' intron1 intron2 intron5 intron3 intron4 exon: ( ) intron: exon( ) exon 5' UTR: 5' mRNA ( ) 3' UTR: 3' mRNA ( ) coding sequence (CDS): ( ) ( ) 1) exon1 exon6 mRNA (transcrip[on) 2) exon ( splicing ) 3) (transla[on) alterna[ve splicing: exon splicing exon exon1+2+3+4+5+6 exon1+2+5+6 exon1+2+3+6
- 14. ‐ (omics)‐ (omics) (genome) (DNA) (transcpritome) (mRNA) gene + ome transcript + ome omics (proteome) ( ) (metabolome) ( ) (phenome) ( )
- 15. DNA GenBank( ) EMBL( ) DDBJ( ) UCSC( ) (SNPs) dbSNP( ) JSNP( ) OMIM( ) Muta[on database( ) SWISS‐PROT( ) PIR( ) Pfam( ) PROSITE( ) BLOCKS( ) PDB( ) SCOP( ) CATH( ) KEGG( ) MEDLINE( ) NCBI( ) Ensembl( )
- 16. PC Mac OS MacBook Pro – ‐ Dock gcc (Xcode) X11 CotEditor MacPorts Unarchiver
- 17. Mac OS Mac OS
- 18. (1)
- 19. (2) ( 10~20 )
- 20. Mac OS ( ) Mac OS
- 21. Finder Finder Macintosh HD Finder Finder : : :
- 22. Mac Mac OS X dock dock dock Finder Safari ( ) Safari
- 23. (1) Machintosh HD/ /Symantec Solu[ons Live Update
- 24. (2) Symantec Scheduler
- 25. (3) Mac Symantec An[Virus 10.1.1 for Macintosh
- 26. (4)
- 27. (5) 3
- 28. (1) Machintosh HD/ /Symantec Solu[ons Symantec An[virus Macintosh HD
- 29. (2) Symantec An[Virus Symantec An[Virus
- 30. MacBook Pro – ‐ Dock Dock Finder
- 31. Dock Macintosh HD/ / Dock ( Dock )
- 32. gcc (Xcode) (1) MacBook Pro MacBook Pro Mac OS X Install Disc 1 MacBook Pro Mac OS X Install DVD Xcode (IDE) gcc (GNU Compiler Collec[on) gcc C C++ Java
- 33. gcc (Xcode) (2‐1) Op[onal Installs → Xcode Tools → Xcode Tools.mpkg Xcode Tools.mpkg
- 34. gcc (Xcode) (2‐2) ( MacBook Pro ) → Xcode Tools → Xcode Tools Xcode Tools
- 35. gcc (Xcode) (3‐1)
- 36. gcc (Xcode) (3‐2) ( MacBook Pro )
- 37. gcc (Xcode) (4)
- 38. X11 (1‐1) MacBook Pro Mac OS X Install Disc 1 (Xcode ) Op[onal Installs → Op[onal Installs.mpkg X11 MacOSX X Window System X Window System XWS UNIX GUI X
- 39. X11 (1‐2) ( MacBook Pro ) MacBook Pro Mac OS X Install Disc 1 (Xcode ) → Op[onal Installs X11 MacOSX X Window System X Window System XWS UNIX GUI X
- 40. X11 (2)
- 41. X11 (3‐1) X11 *
- 42. X11 (3‐2) hop://xquartz.macosforge.org/ XQuartz 2.5.0 released ‐> Download X11‐2.5.0.dmg (Leopard)
- 43. X11 (4)
- 44. (CotEditor) (1) (CotEditor) 5 (2009 5 1 CotEditor 1 0 1)
- 45. (CotEditor) (2) 101 CotEditor
- 46. MacPorts (1) UNIX MacPorts (2010 4 19 MacPorts‐1.8.2)
- 47. MacPorts (2)
- 48. MacPorts (3) PATH $echo $PATH [enter return ] $ /opt/local/bin (:) PATH $ export PATH=$PATH:/opt/local/bin ( page , page ) $echo $PATH PATH
- 49. MacPorts (4) port /opt/local/bin/ /opt/local/bin/ OS *
- 50. (1) tg04
- 51. (2) Unarchiver 8
- 52. PC ‐ ‐ UNIX (NCBI Ensembl) viewer