2 D stage for inverted microscope
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This document summarizes a low-cost 2D stage system designed for use with an inverted microscope. The stage uses a piezo stage with 0.06nm resolution for precise X-Y positioning. Linear actuators provide 100nm resolution over 28mm of travel in the X and Y dimensions. The total cost of the stage was around $2300, without the piezo stage. A joystick and LabVIEW software were used to control the stage and allow efficient scanning of large sample areas under the microscope. The stage was designed to support experiments using optical tweezers to manipulate and study DNA samples.
2 D stage for inverted microscope
- 1. 2D Stage for Inverted Microscope (Economy) Introducing a 2D (X-Y) Joystick controlled stage I designed and built for Optical Tweezers, but could be used with any inverted microscope. This was a low budget stage system composed of parts from different vendors. The X-Y linear stage had 100nm and X-Piezo stage had .06nm resolution. The stage cost around $2300 (without piezo). Youtube: https://youtu.be/3hyVFVzXgvY
- 2. Stage Assembly • Sample slide holder • X-Piezo stage: MAD CITY LABS:- Nano OP-30, 30µm travel .06nm resolution • Miniature Linear Actuators: ZABER:- T-LA28A, 28mm travel 100nm resolution • X-Y linear stage: NEWPORT:- 460A-XY, 20mm travel • 2D Platform: THORLABS, 1.5 inches displacement
- 3. Stage Components Sample holder stage designed by me Piezo stage Adapter Piezo stage was mounted on the platform with an adapter This was used to hold a slide right above the objective. The holder was attached to the piezo stage with 4 rubber pads in between. The pads were used to control Tip-Tilt. X-Piezo Stage
- 4. X-Y stage can be prepared from a single 2D stage or two 1D stages by replacing the micrometers with actuators. X-Y stage Two linear stages Stage Components The stage was mounted on X- Y platform, where two plates were installed for rough adjustment in X-Y to align the slide center with objective optical axis. A sturdy and high enough platform was a requirement of the setup (Olympus IX71 Microscope).
- 6. Control Software GUI I used LabVIEW to write the software to control the stages with a Logitech Attack 3 Joystick. Zaber provides a good library of the drivers. The DNA samples I was using had at least 10K tethers distributed over 15X15mm slide. For experiments I had to find the right tethers among all by scanning the sample under the microscope. It was not a very easy task but this stage and the software made it easy.
- 7. Optical Tweezers designed and built by Pranav Rathi (PhD) This was my PhD project: Optical Tweezers for Shotgun DNA mapping (sequencing) and DNA overstretching in water isotopes.