B4FA 2012 Ghana: Transgenic Crop Projects in Ghana - IDK Atokple
- 1. BtBt--Cotton ProjectCotton Project Biosafety Testing ofBiosafety Testing of ‘‘HighHigh--ProteinProtein’’ SweetSweet potato in Ghanapotato in Ghana Nitrogen Use Efficiency and Salt Tolerance inNitrogen Use Efficiency and Salt Tolerance in RiceRice MarucaMaruca--resistant Cowpea Project.resistant Cowpea Project.
- 2. Biosafety Testing ofBiosafety Testing of ‘‘HighHigh--ProteinProtein’’ Sweet potato in GhanaSweet potato in Ghana Collaborative Research TuskegeeCollaborative Research Tuskegee University, AL, USA /CSIRUniversity, AL, USA /CSIR––CRICRI STARTED 2008STARTED 2008STARTED 2008STARTED 2008
- 3. Sweetpotato is a major source of energy, minerals, and vitamins In West Africa, sweetpotato is gradually becoming an important crop because of its potential as a food and industrial crop, and can be produced in almost all the agro-ecological zones in the sub- region. Sweetpotato is relatively poor in essential amino acids with tryptophan, methionine, and Lysine being the most limiting and to a lesser extent isoleucine, theorine and leucine. An increase in these essential amino acids in the crop will be more beneficial.
- 4. H I J K L M A B C D E F G N P
- 5. INCREASE IN PROTEIN CONTENTINCREASE IN PROTEIN CONTENT Sweetpotato plants engineered with the ASP1 gene showed a Three to Five Fold Increase in the protein content
- 6. 0 100 200 300 400 500 600EAAContent Ile Leu Lys Met Phe Thr Trp Val Control TA3
- 7. Accomplishments Enhanced protein content Increased overall Essential Amino Acids (EAA) No yield penalty in Transgenic Sweet potato lines slide 6
- 9. Maruca-Resistant Cowpea Project Funded by Africa Agricultural Technology Foundation, (AATF), Nairobi, Kenya
- 11. Pod damage
- 12. IITA and NARS in West Africa dedicated over 30 years to cowpea research and development. Breeding for pest, disease and abiotic stresses Insect pests: Aphids, flower thrips, pod borers, pod -sucking bugs, bruchids.
- 13. Establishment of T0 plantlets in soil Successful Genetic transformation - stable
- 14. Single Copy inserts: Southern blotting Integrity of inserts: DNA sequencing Cry1Ab gene transmits to next generation and segregates in Bt cowpeas Bt protein is expressed in green tissues (pods, young seeds), pollen etc
- 15. 81% Survival 0% Survival 0% Survival Control cv. IT86D Tg1 67A5-11 Tg2 67A5-14 Non Transgenic Transgenic Cowpea
- 16. Bt cowpeas are protected from Helicoverpa armigera
- 17. Bt cowpea is highly toxic to H.armigera Non Transgenic Transgenic Line Transgenic Line Transgenic Line Transgenic Line Transgenic Line Transgenic Line Transgenic Line Transgenic Line Transgenic Line Transgenic Line Transgenic Line IT86D-1010 CP81A 705A 705C 705D 703C 705B 705F 706A 706B 706C 710B % Mortality 6% 100% 100% 94% 94% 100% 100% 83% 100% 100% 93% 88%
- 19. • Formation of the IBC • Research Team • Training in Field Management Skills? • Logistics/Infrastructure
- 22. Layout of the CFT site iin Offic e and store house Experimental FieldExperimental Field Tanks Pit SH Screen house Drying Platfor m
- 24. 24 Front view of Confined Field Trial Site, CSIR-SARI
- 25. Ghana has signed the Cartagena Protocol on Biosafety The national biosafety framework and regulations have been developed. Environment safety : testing of Bt cowpea must be conducted according to international and national regulations Ensures food and feed safety
- 28. Application Permit Planting Harvest/Termination Completion Technical Review Regulatory Decision Legal Authorization With Terms & Conditions Regulatory Inspections Current Season Post-Harvest Period
- 29. Aim to: Prevent dissemination of new genes into the environment (i.e., prevent pollen flow) Prevent the persistence in the environment of the experimental biotech plants and progeny (i.e., contain seed and planting material) Prevent introduction of the experimental material into livestock feed or human food pathways
- 30. Template for Risk Assessment of Activities with Transgenic Organisms Summary of Considerations Date: ________________________ Application No.: _____________________________________________________________________ Transgenic organism: _________________________________________________________________ Activity: Confined / Contained / General release / Import / Export / ____________________________ Concern Potential hazard Likelihood Consequence Management? Acceptable risk? Risk Assessment recommendation: _________________________________________________________________________________________ Terms and Conditions of the approved use attached: Yes / No Names and signatures of reviewers:
- 31. AATF-PBS training effort on biosafety & regulatory compliance
- 32. Maruca Resistant Cowpea Project EnablingFunctions IP and Licensing Public Acceptance and Communication Regulatory Science and Regulatory Affairs Activities Biosafety Assessment Approval Strategy formulation Permit application for efficacy testing Permit applications Environ. safety evaluation Feed/food safety assessment Compositional analysis Permit applications Agronomic trials Variety testing Seed increase Interaction with regulatory agencies Seek permit for commercial release Develop specific detection kits Develop resistant management plans Efficacy testing Elite event selection Agronomic trials Proof of Concept TechDevelopment Protocol optimization Event generation Mol. charaterization Trait integration Variety dev. & release Seed bulk- up Commercial mktg. Early Prod Dev. DeploymentAdv. Pr. Dev. S T E W A R D S H I P
- 33. Developed and submit a confined field trial application to NBC Implement confined field trial of these crops to select successful lines for further use.