© Boardworks Ltd 2007
1 of 42
© Boardworks Ltd 2007
2 of 42
© Boardworks Ltd 2007
3 of 42
What’s the missing link?
What is the link between digestion, photosynthesis, bread,
wine and washing powder?
They all depend on enzymes.
© Boardworks Ltd 2007
4 of 42
What are enzymes?
Enzymes are biological catalysts – they speed up the
chemical reactions that take place inside all cells, but
without being used up in the process.
There are many thousands of different types of enzyme,
and each one catalyzes a different reaction.
Enzymes occur naturally in all organisms, but they are
increasingly being used in industrial processes.
© Boardworks Ltd 2007
5 of 42
What are enzymes made of?
Enzymes are protein molecules, and so are made up of
amino acids. Most enzymes contain between 100 and
1,000 amino acids.
These amino acids are joined together in a long chain,
which is folded to produce a unique 3D structure.
© Boardworks Ltd 2007
6 of 42
Why is shape important?
The shape of an enzyme is very important because it has
a direct effect on how it catalyzes a reaction.
An enzyme’s shape is
determined by the sequence of
amino acids in its structure, and
the bonds which form between
the atoms of those molecules.
Why do enzymes have different shapes?
Different types of enzymes have different shapes and
functions because the order and type of amino acids in
their structure is different.
© Boardworks Ltd 2007
7 of 42
Enzymes: true or false?
© Boardworks Ltd 2007
8 of 42
© Boardworks Ltd 2007
9 of 42
Fussy enzymes?
Why are enzymes so specific in their reactions?
© Boardworks Ltd 2007
10 of 42
Why are enzymes so specific?
Enzymes are very specific about which reactions they
catalyze. Only molecules with exactly the right shape will
bind to the enzyme and react. These are the reactant, or
substrate, molecules.
The part of the enzyme to
which the reactant binds is
called the active site.
This is a very specific shape
and the most important part
of the enzyme.
© Boardworks Ltd 2007
11 of 42
What happens at the active site?
In the same way that a key fits into a lock, so a substrate
is thought to fit into an enzyme’s active site. The enzyme
is the lock, and the reactant is the key.
enzyme
reactant
+
enzyme-reactant
complex
↔
products
enzyme
+
↔
+ ↔ ↔ +
© Boardworks Ltd 2007
12 of 42
The lock and key model
© Boardworks Ltd 2007
13 of 42
Why do enzymes speed up reactions?
Enzymes speed up reactions by lowering the activation
energy (Ea) of a reaction. The activation energy is the
energy needed to start a reaction.
Different reactions have different activation energies.
reaction (time)
energy
(kJ)
Ea with enzyme
Ea without enzyme
© Boardworks Ltd 2007
14 of 42
Factors affecting enzymes
The rate of enzyme–catalyzed reactions depends on
several factors. What are some of these?
All enzymes work best at only one particular temperature
and pH: this is called the optimum.
Factors that affect the rate of a reaction include:
 substrate concentration
 temperature
Different enzymes have different optimum temperatures
and pH values.
 pH
 enzyme concentration
 surface area
 pressure.
© Boardworks Ltd 2007
15 of 42
Factors affecting enzymes
If the temperature and pH changes sufficiently beyond an
enzyme’s optimum, the shape of the enzyme irreversibly
changes.
normal denatured
heat
pH
This affects the shape of the active site and means that
the enzyme will no longer work.
When this happens the enzyme is denatured.
© Boardworks Ltd 2007
16 of 42
Enzymes and temperature
© Boardworks Ltd 2007
17 of 42
Enzyme inhibitors
© Boardworks Ltd 2007
18 of 42
Identifying enzyme terms
© Boardworks Ltd 2007
19 of 42
© Boardworks Ltd 2007
20 of 42
Enzymes and cells
Enzymes catalyze the thousands of reactions that need to
take place in order to maintain life. What are some of
these reactions?
 digestion
 respiration
 photosynthesis
(plants and some bacteria)
 protein synthesis.
© Boardworks Ltd 2007
21 of 42
What are digestive enzymes?
Not all enzymes work inside cells. In what process do
enzymes work outside cells?
Here the enzymes help
to break down large food
molecules into smaller
molecules that are more
easily absorbed.
Digestive enzymes are produced by specialized cells in
the pancreas and digestive tract.
These enzymes pass
out of the cells and into
the stomach and small
intestine.
© Boardworks Ltd 2007
22 of 42
Enzymes of digestion
© Boardworks Ltd 2007
23 of 42
Digestion in the stomach
When food enters the stomach it stimulates the secretion
of hydrochloric acid (HCl) from the stomach wall. HCl
increases the acidity of the stomach to about pH2 – the
optimum pH for stomach enzymes.
oesophagus
duodenum
mucus cells
parietal cells
(acid-producing)
gastric gland
© Boardworks Ltd 2007
24 of 42
Digestion in the small intestine
The liver produces bile (an alkali), which is stored in the
gall bladder and released into the small intestine.
Bile neutralizes the
acidic contents coming
from the stomach,
creating the alkaline
environment that the
intestinal enzymes
need to work.
Digestive enzymes found in the small intestine are damaged
by a strongly acidic pH.
How does the body avoid this problem?
gall
bladder
duodenum bile duct
pancreas
hepatic
duct
© Boardworks Ltd 2007
25 of 42
What is aerobic respiration?
Aerobic respiration is a series of reactions that release
energy from glucose using oxygen.
Aerobic respiration can be summarized by this equation:

+ oxygen carbon dioxide water
glucose + + energy
In animals, plants and
fungi, most of the reactions
in aerobic respiration take
place in mitochondria.
Mitochondria are the energy-
producing part of the cell.
© Boardworks Ltd 2007
26 of 42
© Boardworks Ltd 2007
27 of 42
Enzymes in the home
How many items are made using enzymes?
© Boardworks Ltd 2007
28 of 42
Breadmaking
© Boardworks Ltd 2007
29 of 42
Making alcohol
© Boardworks Ltd 2007
30 of 42
Confectionary
A type of enzyme called isomerase converts the sugar
glucose into fructose, another type of sugar.
Fructose is sweeter than glucose, so a smaller amount is
needed. This makes fructose syrup a useful ingredient in
slimming foods.
Invertase is used to create soft-centered chocolates. The
centre initially contains sucrose (cane sugar) and is hard.
The invertase breaks
down the sucrose into the
simpler sugars glucose
and sucrose, making the
centre soft and runny.
© Boardworks Ltd 2007
31 of 42
How do enzymes help to clean clothes?
The enzymes are coated with a special wax that melts in the
wash, releasing the enzymes. Once the stains have been
broken down, they are easier for the detergent to remove.
Biological washing powders and liquids contain enzymes
that help remove stains.
 Proteases break down proteins
in stains such as grass, blood
and sweat.
 Lipases break down stains
containing fat and oil.
 Carbohydrases break down
carbohydrate-based stains,
such as starch.
© Boardworks Ltd 2007
32 of 42
Producing enzymes
Many of the enzymes used in the home and in industry are
naturally produced by micro-organisms such as bacteria
and yeast.
These enzymes are very useful in manufacturing processes
because they catalyze reactions in a very predictable way
and can replace harmful chemical supplements.
Bacteria and yeast can
be manipulated and/or
genetically engineered to
produce these enzymes
on an industrial scale.
This process is called
fermentation. bacteria
© Boardworks Ltd 2007
33 of 42
Fermentation
© Boardworks Ltd 2007
34 of 42
Production processes
Enzymes are used in two main types of industrial process:
 batch production – reactants are converted into
products, and the enzymes are destroyed in the process.
Immobilized enzymes are attached to unreactive materials
such as alginate beads (derived from seaweed).
 continuous production – a constant stream of
reactants is passed over immobilized enzymes so
products are continuously formed. The enzymes are
not destroyed.
What are the advantages and disadvantages of batch
and continuous production?
© Boardworks Ltd 2007
35 of 42
Are enzymes the future?
© Boardworks Ltd 2007
36 of 42
© Boardworks Ltd 2007
37 of 42
Glossary (1/2)
activation energy – The energy needed to start a
chemical reaction.
active site – The part of the enzyme into which the
reactant molecule fits.
catalyst – A substance that changes the rate of a
reaction without being used up.
denatured – The state of an enzyme when it has been
irreversibly damaged and has changed shape.
enzyme – A biological catalyst.
fermentation – The conversion of sugar to ethanol and
carbon dioxide by enzymes in yeast.
© Boardworks Ltd 2007
38 of 42
Glossary (2/2)
inhibitor – A molecule that decreases the rate of
enzyme-catalysed reactions.
lock and key – A model of how enzymes work and the
importance of their shape.
optimum – The temperature and pH at which an
enzyme-catalyzed reaction proceeds at the fastest rate.
reactant – The molecule that binds to and reacts with
the enzyme.
respiration – The series of reactions that release
energy from glucose, in the presence of oxygen.
© Boardworks Ltd 2007
39 of 42
Anagrams
© Boardworks Ltd 2007
40 of 42
Match the reactant
© Boardworks Ltd 2007
41 of 42
Name the structure
© Boardworks Ltd 2007
42 of 42
Multiple-choice quiz

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7. Enzymes v1.0.ppt boardworks powerpoint

  • 1. © Boardworks Ltd 2007 1 of 42
  • 2. © Boardworks Ltd 2007 2 of 42
  • 3. © Boardworks Ltd 2007 3 of 42 What’s the missing link? What is the link between digestion, photosynthesis, bread, wine and washing powder? They all depend on enzymes.
  • 4. © Boardworks Ltd 2007 4 of 42 What are enzymes? Enzymes are biological catalysts – they speed up the chemical reactions that take place inside all cells, but without being used up in the process. There are many thousands of different types of enzyme, and each one catalyzes a different reaction. Enzymes occur naturally in all organisms, but they are increasingly being used in industrial processes.
  • 5. © Boardworks Ltd 2007 5 of 42 What are enzymes made of? Enzymes are protein molecules, and so are made up of amino acids. Most enzymes contain between 100 and 1,000 amino acids. These amino acids are joined together in a long chain, which is folded to produce a unique 3D structure.
  • 6. © Boardworks Ltd 2007 6 of 42 Why is shape important? The shape of an enzyme is very important because it has a direct effect on how it catalyzes a reaction. An enzyme’s shape is determined by the sequence of amino acids in its structure, and the bonds which form between the atoms of those molecules. Why do enzymes have different shapes? Different types of enzymes have different shapes and functions because the order and type of amino acids in their structure is different.
  • 7. © Boardworks Ltd 2007 7 of 42 Enzymes: true or false?
  • 8. © Boardworks Ltd 2007 8 of 42
  • 9. © Boardworks Ltd 2007 9 of 42 Fussy enzymes? Why are enzymes so specific in their reactions?
  • 10. © Boardworks Ltd 2007 10 of 42 Why are enzymes so specific? Enzymes are very specific about which reactions they catalyze. Only molecules with exactly the right shape will bind to the enzyme and react. These are the reactant, or substrate, molecules. The part of the enzyme to which the reactant binds is called the active site. This is a very specific shape and the most important part of the enzyme.
  • 11. © Boardworks Ltd 2007 11 of 42 What happens at the active site? In the same way that a key fits into a lock, so a substrate is thought to fit into an enzyme’s active site. The enzyme is the lock, and the reactant is the key. enzyme reactant + enzyme-reactant complex ↔ products enzyme + ↔ + ↔ ↔ +
  • 12. © Boardworks Ltd 2007 12 of 42 The lock and key model
  • 13. © Boardworks Ltd 2007 13 of 42 Why do enzymes speed up reactions? Enzymes speed up reactions by lowering the activation energy (Ea) of a reaction. The activation energy is the energy needed to start a reaction. Different reactions have different activation energies. reaction (time) energy (kJ) Ea with enzyme Ea without enzyme
  • 14. © Boardworks Ltd 2007 14 of 42 Factors affecting enzymes The rate of enzyme–catalyzed reactions depends on several factors. What are some of these? All enzymes work best at only one particular temperature and pH: this is called the optimum. Factors that affect the rate of a reaction include:  substrate concentration  temperature Different enzymes have different optimum temperatures and pH values.  pH  enzyme concentration  surface area  pressure.
  • 15. © Boardworks Ltd 2007 15 of 42 Factors affecting enzymes If the temperature and pH changes sufficiently beyond an enzyme’s optimum, the shape of the enzyme irreversibly changes. normal denatured heat pH This affects the shape of the active site and means that the enzyme will no longer work. When this happens the enzyme is denatured.
  • 16. © Boardworks Ltd 2007 16 of 42 Enzymes and temperature
  • 17. © Boardworks Ltd 2007 17 of 42 Enzyme inhibitors
  • 18. © Boardworks Ltd 2007 18 of 42 Identifying enzyme terms
  • 19. © Boardworks Ltd 2007 19 of 42
  • 20. © Boardworks Ltd 2007 20 of 42 Enzymes and cells Enzymes catalyze the thousands of reactions that need to take place in order to maintain life. What are some of these reactions?  digestion  respiration  photosynthesis (plants and some bacteria)  protein synthesis.
  • 21. © Boardworks Ltd 2007 21 of 42 What are digestive enzymes? Not all enzymes work inside cells. In what process do enzymes work outside cells? Here the enzymes help to break down large food molecules into smaller molecules that are more easily absorbed. Digestive enzymes are produced by specialized cells in the pancreas and digestive tract. These enzymes pass out of the cells and into the stomach and small intestine.
  • 22. © Boardworks Ltd 2007 22 of 42 Enzymes of digestion
  • 23. © Boardworks Ltd 2007 23 of 42 Digestion in the stomach When food enters the stomach it stimulates the secretion of hydrochloric acid (HCl) from the stomach wall. HCl increases the acidity of the stomach to about pH2 – the optimum pH for stomach enzymes. oesophagus duodenum mucus cells parietal cells (acid-producing) gastric gland
  • 24. © Boardworks Ltd 2007 24 of 42 Digestion in the small intestine The liver produces bile (an alkali), which is stored in the gall bladder and released into the small intestine. Bile neutralizes the acidic contents coming from the stomach, creating the alkaline environment that the intestinal enzymes need to work. Digestive enzymes found in the small intestine are damaged by a strongly acidic pH. How does the body avoid this problem? gall bladder duodenum bile duct pancreas hepatic duct
  • 25. © Boardworks Ltd 2007 25 of 42 What is aerobic respiration? Aerobic respiration is a series of reactions that release energy from glucose using oxygen. Aerobic respiration can be summarized by this equation:  + oxygen carbon dioxide water glucose + + energy In animals, plants and fungi, most of the reactions in aerobic respiration take place in mitochondria. Mitochondria are the energy- producing part of the cell.
  • 26. © Boardworks Ltd 2007 26 of 42
  • 27. © Boardworks Ltd 2007 27 of 42 Enzymes in the home How many items are made using enzymes?
  • 28. © Boardworks Ltd 2007 28 of 42 Breadmaking
  • 29. © Boardworks Ltd 2007 29 of 42 Making alcohol
  • 30. © Boardworks Ltd 2007 30 of 42 Confectionary A type of enzyme called isomerase converts the sugar glucose into fructose, another type of sugar. Fructose is sweeter than glucose, so a smaller amount is needed. This makes fructose syrup a useful ingredient in slimming foods. Invertase is used to create soft-centered chocolates. The centre initially contains sucrose (cane sugar) and is hard. The invertase breaks down the sucrose into the simpler sugars glucose and sucrose, making the centre soft and runny.
  • 31. © Boardworks Ltd 2007 31 of 42 How do enzymes help to clean clothes? The enzymes are coated with a special wax that melts in the wash, releasing the enzymes. Once the stains have been broken down, they are easier for the detergent to remove. Biological washing powders and liquids contain enzymes that help remove stains.  Proteases break down proteins in stains such as grass, blood and sweat.  Lipases break down stains containing fat and oil.  Carbohydrases break down carbohydrate-based stains, such as starch.
  • 32. © Boardworks Ltd 2007 32 of 42 Producing enzymes Many of the enzymes used in the home and in industry are naturally produced by micro-organisms such as bacteria and yeast. These enzymes are very useful in manufacturing processes because they catalyze reactions in a very predictable way and can replace harmful chemical supplements. Bacteria and yeast can be manipulated and/or genetically engineered to produce these enzymes on an industrial scale. This process is called fermentation. bacteria
  • 33. © Boardworks Ltd 2007 33 of 42 Fermentation
  • 34. © Boardworks Ltd 2007 34 of 42 Production processes Enzymes are used in two main types of industrial process:  batch production – reactants are converted into products, and the enzymes are destroyed in the process. Immobilized enzymes are attached to unreactive materials such as alginate beads (derived from seaweed).  continuous production – a constant stream of reactants is passed over immobilized enzymes so products are continuously formed. The enzymes are not destroyed. What are the advantages and disadvantages of batch and continuous production?
  • 35. © Boardworks Ltd 2007 35 of 42 Are enzymes the future?
  • 36. © Boardworks Ltd 2007 36 of 42
  • 37. © Boardworks Ltd 2007 37 of 42 Glossary (1/2) activation energy – The energy needed to start a chemical reaction. active site – The part of the enzyme into which the reactant molecule fits. catalyst – A substance that changes the rate of a reaction without being used up. denatured – The state of an enzyme when it has been irreversibly damaged and has changed shape. enzyme – A biological catalyst. fermentation – The conversion of sugar to ethanol and carbon dioxide by enzymes in yeast.
  • 38. © Boardworks Ltd 2007 38 of 42 Glossary (2/2) inhibitor – A molecule that decreases the rate of enzyme-catalysed reactions. lock and key – A model of how enzymes work and the importance of their shape. optimum – The temperature and pH at which an enzyme-catalyzed reaction proceeds at the fastest rate. reactant – The molecule that binds to and reacts with the enzyme. respiration – The series of reactions that release energy from glucose, in the presence of oxygen.
  • 39. © Boardworks Ltd 2007 39 of 42 Anagrams
  • 40. © Boardworks Ltd 2007 40 of 42 Match the reactant
  • 41. © Boardworks Ltd 2007 41 of 42 Name the structure
  • 42. © Boardworks Ltd 2007 42 of 42 Multiple-choice quiz

Editor's Notes

  • #3: Photo credit: © 2007 Jupiterimages Corporation
  • #7: Teacher notes This true-or-false activity could be used as a plenary or revision exercise on enzymes, or at the start of the lesson to gauge students’ existing knowledge of the subject matter. Coloured traffic light cards (red = false, yellow = don’t know, green = true) could be used to make this a whole-class exercise.
  • #9: Teacher notes This illustration contains several discussion points relating to enzymes, including: Thermostat Different enzymes have different optimum temperatures. Beyond their optimum temperature enzymes denature (breakdown). The thermostat in the illustration is set to a high temperature, and most of the enzymes seem uncomfortably hot. Arguments The shape of an enzyme’s active site determines which reactants it can bond with. In this illustration, incompatible enzyme–reactant pairs are shown either arguing or ignoring each other. ‘The happy couple’ Enzymes and reactants that are compatible are said to fit together like a ‘lock and key’. The happy couple shown in the bottom left of the illustration have corresponding shapes, and seem to be reacting well to each other in the warm environment.
  • #10: Photo credit: JC Revy / Science Photo Library The image shows a molecular computer graphics image of ribonuclease A, an enzyme involved in the destruction of messenger RNA (mRNA) in the cytoplasm of bacteria. At left is its substrate, shifted away from the active site. Enzymes are biological catalysts, proteins that speed up the rates of reactions within cells. Each enzyme is specific for a particular reaction; interaction occurs (typically as a weak bond) between an active site on the enzyme & a reactant (or substrate) due to the arrangement of mutually attractive groups of atoms. This image displays the molecular surface (blue) & polypeptide chain: colours are used to represent the polarity of constituent amino acids.
  • #12: Teacher notes This four-stage animation demonstrates the principles of the ‘lock and key’ model. While showing the animation, the specific shape of the active site could be highlighted. Suitable prompts could include: What is special about the shape of the active site? Is the enzyme the ‘lock’ or the ‘key’?
  • #13: Teacher notes See the ‘Energy Transfer’ and ‘Rates of Reaction’ chemistry presentations for more information on activation energy.
  • #16: Teacher notes This five-stage animation could be used as a precursor to running the experiment in the lab, or as a revision exercise.
  • #17: Teacher notes This four-stage animation shows how competitive and non-competitive inhibitors block the action of enzymes by either binding to, or altering the shape of, their active site.
  • #18: Teacher notes This matching activity could be used as a plenary or revision exercise on enzymes. Students could be asked to complete the questions in their books and the activity could be concluded by the completion on the IWB.
  • #22: Teacher notes This activity could be used to check students’ understanding of chemical digestion.
  • #25: Teacher notes See the ‘Animal and Plant Cells’ presentation for more information about mitochondria and respiration.
  • #27: Teacher notes This illustration contains several discussion points relating to items made using enzymes, including: Baby food: Proteases such as trypsin are used to predigest the protein in baby foods. Baking (bread): Fungal enzymes are used to catalyze the breakdown of starch into sugar. The enzymes also produce carbon dioxide gas, which makes the dough rise. Biological detergent (washing powder): Proteases, amylases and lipases are used to remove protein, starch and oily stains from clothes. Brewing (beer): Proteases in barley are released as it is fermented during beer production. The enzymes break-down starch, carbohydrases and proteins, and clarify stored beers. Confectionary (chocolates): Enzymes are used to make soft-centred chocolates. Dairy products (cheese): Rennet (an enzyme from calves’ stomachs) is used to coagulate milk during cheese production, separating the curd (solids) from the whey (liquid). Lipases are used to enhance the flavour and ripening of blue cheeses, and lactases are used to break down-lactose into glucose and galactose. Fruit juice: Enzymes that act on cellulose reduce the cloudiness of fruit juices. Paper (penicillin box): Proteases and cellulases are used to soften and smooth pulped fibres during paper manufacture Photography: Proteases are used to dissolve gelatin from waste film, allowing its silver content to be recovered and recycled. Rubber (place mat in high chair): Catalase is used to convert latex into foam rubber.
  • #28: Teacher notes This three-stage animation shows how enzymes are used in breadmaking. Suitable prompts could include: What happens to the amylase and invertase during the baking process? What is the name of the gas that the yeast produce during fermentation?
  • #29: Teacher notes This activity could be used as a precursor to a wider discussion on drugs use and abuse. See the GCSE Science (Biology) ‘Drug Use and Abuse’ presentation for more information on alcohol.
  • #35: Teacher notes Appropriately coloured voting cards could be used with this classification activity to increase class participation.
  • #40: Teacher notes This matching activity could be used as a plenary or revision exercise on enzymes. Students could be asked to complete the questions in their books and the activity could be concluded by the completion on the IWB.
  • #41: Teacher notes This activity could be used to check students’ understanding of the lock and key model.
  • #42: Teacher notes This multiple-choice quiz could be used as a plenary activity to assess students’ understanding of enzymes. The questions can be skipped through without answering by clicking “next”. Students could be asked to complete the questions in their books and the activity could be concluded by the completion on the IWB.