AEC COUNT
- 1. AEC Ms Ankita R Bhatiya Assistant Professor Shree P.M.Patel COLLEGE OF PARAMEDICAL SCIENCE N TECHNOLOGY
- 2. It include: 1.What is AEC? 2. Method for detection of AEC.
- 3. AEC Introduction: Definition: An absolute eosinophil count is a blood test that measures the number of one type of white blood cells called eosinophils. Eosinophils become active when you have certain allergic diseases, infections, and other medical conditions.
- 4. AEC Aim: To determination of absolute Eosinophil count Clinical Significance: Eosinophil count has a close relationship with the adrenal function & Eosinophil count can be diagnostic for adrenal related disorder. Increased Eosinophil count is often associated with allergic condition, parasitic infection & certain leukaemia etc. Increase in the adrenal function (hyperadrenalism or Cushing's syndromes) is associated with a fall in Eosinophil count. Method: Haemocytometry method
- 5. AEC Normal Range: 40 - 440 /cumin Principle: AEC diluting fluid is called Hingleman's solution. Blood is diluted 1:10 with a special diluting fluid, which remove red cells & stains the eosinophil red. These cells are then counted under low power in a known volume of fluid by using counting chamber. Requirements: Microscope, Neubauer's chamber, Hb pipette, cotton Test tube, diluting fluid Diluting fluid contains: - Yellow eosin (0.5g), 95% phenol (0.5m1), formalin (0.5m1), Distilled Water (99m1). Sample: EDTA blood sample
- 6. AEC Procedure: 1. Identify the right patient. 2.Collect all the requirements. 3. Do the labelling. 4. Collect require amount of blood by capillary or vein puncture. 5.In a labelled test tube take 0.36 ml of AEC diluting fluid. 6. Add 0.04 ml of blood (use Hb pipette, twice). 7. Mix it well & keep it for 10 min. 8. Charge the Neubauer's chamber & let it stand under a moist Petridis for about 2 to 3 min. 9. Count the Eosinophil under the low power of microscope in all the nine squares. 10. Do calculation. 11.Clean the working area 12. Put all the requirements at them of place 13. Wash the hand.
- 7. AEC Calculation: Precaution: Eosinophil disintegrates in the diluting fluid hence the count should not be delayed for more than 30 minute after diluting the blood.