The document studies the effect of cyclic prefix (CP) length on inter-symbol interference (ISI) in OFDM systems using 16 QAM modulation, highlighting its impact on bit error rate (BER) in both Gaussian and Rayleigh noise environments. Through MATLAB simulations, it establishes that the CP mitigates ISI and inter-carrier interference (ICI) by transforming linear convolution into discrete forms, thus improving signal reception. The paper provides analytical models and simulation results to demonstrate the efficiency of CP in realistic communication scenarios.