Bacterial persistence
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This document discusses bacterial persistence, where a subpopulation of dormant bacterial cells called persisters can tolerate antibiotic treatment without being killed. Persisters enter a dormant, non-dividing state that makes them tolerant to antibiotics. They were first discovered in 1944 when it was found that penicillin could kill 99% of bacteria but 1% persisted. Current hypotheses suggest persisters form due to toxin-antitoxin modules that inhibit cellular functions. Persistence differs from antibiotic resistance as persisters are still susceptible to antibiotics when returning to an active state. Persisters are important for multi-drug tolerance, biofilm formation, and chronic infections like tuberculosis.
Bacterial persistence
- 1. Bacterial Persistence Instructor: Dr. Wu, Jia-Feng Chu, Yung-Tsai 2011.01.02
- 2. Outline What is Bacterial Persistence Possible Mechanism Comparison with Antibiotics Resistance Importance Take Home Message
- 3. Bacterial Persistence a state in which a sub-population of dormant cells, or ‘persisters’, tolerates antibiotic treatment A dormant cell has a global slowdown of metabolic processes and does not divide
- 4. Discovery in 1944 Joseph BIGGER demonstrated that penicillin can kill merely 99% of the bacteria. The remaining 1% of the bacteria were persisters. When these persisters were cultured to fresh media, they regained susceptibility to antibiotics.
- 5. Implication 1. Certain sub-population can persist under the antibiotic treatment. 2. They are still susceptible to the antibiotics. These cells have not genetically acquired antibiotic resistance.
- 6. Mechanism Growth Dormant (Dividing) (Persister)
- 7. Current Hypothesis Toxin-antitoxin module the toxin is a protein that inhibits an important cellular function
- 10. Importance Multidrug -Tolerance Biofilms Chronic Infection Treponema pallidum (syphilis) M. tuberculosis (TB) Association with antibiotics resistance
- 11. Take Home Message The entrance of cells into a dormant, persistent state is largely responsible for the multidrug tolerance of infections Persisters are likely to be responsible for Multidrug tolerance of biofilms and latent (chronic) diseases, such as TB
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