Biochemistry Spotters
Aarushi Bhardwaj
1. Glass pipette
• to transport a measured volume
of liquid
• 2ml, 5ml, 10ml
• presume tip as 1ml
2. Funnel
• plastic
• used to transfer liquids into
containers that have narrow
mouth
• can also be used a filter, when
filter paper is used with it
3. Borosilicated test tube
• heat resistant
• 13ml
• used for performing experiments
4. Plastic rack
• to keep test tubes
5. Test tube holder
• wood and metal
• has lock and key
6. Borosilicated conical flask
• graduated
• heat resistant
• used for experiments
• put it in a stand
with a mesh and
spirit lamp below
7. Borosil glass beaker
• hold, mix and heat reagents
• 50ml, 100ml, 80ml, 200ml,
500ml, 1000ml, 2000ml
8. Glass measuring cylinder
• 25ml,50ml, 100ml, 1000ml
• A graduated cylinder / cylinder
measuring / mixing cylinder is
used to measure the volume of a
liquids, chemicals or solutions
9. Paper chromatography
• substances are distributed
between a stationary phase and
a mobile phase.When a coloured
sample is placed on a filter
paper, the colours separate from
the sample by placing one end of
the paper in a solvent
10. Litmus paper
• test both for neutral
• red to blue (basic)
• blue to red (acidic)
11. Spatula
• steel
• used to take reagent and mix in
test tube
12. Urinometer
• works on principle of
buoyancy/ upward thrust
• used to measure specific
gravity of urine
• add 0.001 to 16° (move every
3°)
• if temp. < (every 3°) = -0.001
• if temp. > (every 3°) = +0.001
13. Glass cuvette
• take the absorbance/ optical
density (OD) in colorimeter
• fix it on the mark
14. Plastic dropper
• to measure and transport small
amount of reagent or sample
• eg- benedict's test
15. Glass bottle to store reagent
16. Albuminometer
• use overnight and see
sedimentation in morning
• borosilicated
• test tube with cork (red)
17. pH meter
• principle - measures pH onthe
basis of concentration of H+ ions
• measure pH of fluids
• make solution of particular pH
• has AgCl in
electrode
18. Colorimeter
• principle- based on Beer-
Lambert's Law ; when a
monochromatic light passes
through an absorbing medium
the absorbance is directly
proportional to the
concentration of the medium
and the path length
19. Vortex shaker
• to mix small amount of liquids
quickly in circular motion
20. Semi auto analyser
• samples and reagents are mixed
and read manually
• based on colorimetry principle
21. Water bath
• the temp. of water is maintained
at a constant level.
• boiling point of water - 100°C
• it is used to incubate samples
over a period of time at a
constant temp.
22. Glucose tolerance test curves
23. Plasma protein electrophoresis
• A - albumin
• B - alpha 1
• C - alpha 2
• D - Beta globulins
• E - Gamma globulins
• diagnosis - multiple myeloma
(cancer of plasma cells)
• tumor marker - bence jone's
proteins
• abnormal band between beta
globulins and gamma globulins
Benedicts reagent (dark blue)
Sulphur powder
(light yellow)
Biuret reagent (light blue)
Picric acid
(dark yellow)
Thankyou

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Biochemistry Spotters SHOWING VARIOUS INSTRUMENTS

  • 2. 1. Glass pipette • to transport a measured volume of liquid • 2ml, 5ml, 10ml • presume tip as 1ml
  • 3. 2. Funnel • plastic • used to transfer liquids into containers that have narrow mouth • can also be used a filter, when filter paper is used with it
  • 4. 3. Borosilicated test tube • heat resistant • 13ml • used for performing experiments
  • 5. 4. Plastic rack • to keep test tubes
  • 6. 5. Test tube holder • wood and metal • has lock and key
  • 7. 6. Borosilicated conical flask • graduated • heat resistant • used for experiments • put it in a stand with a mesh and spirit lamp below
  • 8. 7. Borosil glass beaker • hold, mix and heat reagents • 50ml, 100ml, 80ml, 200ml, 500ml, 1000ml, 2000ml
  • 9. 8. Glass measuring cylinder • 25ml,50ml, 100ml, 1000ml • A graduated cylinder / cylinder measuring / mixing cylinder is used to measure the volume of a liquids, chemicals or solutions
  • 10. 9. Paper chromatography • substances are distributed between a stationary phase and a mobile phase.When a coloured sample is placed on a filter paper, the colours separate from the sample by placing one end of the paper in a solvent
  • 11. 10. Litmus paper • test both for neutral • red to blue (basic) • blue to red (acidic)
  • 12. 11. Spatula • steel • used to take reagent and mix in test tube
  • 13. 12. Urinometer • works on principle of buoyancy/ upward thrust • used to measure specific gravity of urine • add 0.001 to 16° (move every 3°) • if temp. < (every 3°) = -0.001 • if temp. > (every 3°) = +0.001
  • 14. 13. Glass cuvette • take the absorbance/ optical density (OD) in colorimeter • fix it on the mark
  • 15. 14. Plastic dropper • to measure and transport small amount of reagent or sample • eg- benedict's test
  • 16. 15. Glass bottle to store reagent
  • 17. 16. Albuminometer • use overnight and see sedimentation in morning • borosilicated • test tube with cork (red)
  • 18. 17. pH meter • principle - measures pH onthe basis of concentration of H+ ions • measure pH of fluids • make solution of particular pH • has AgCl in electrode
  • 19. 18. Colorimeter • principle- based on Beer- Lambert's Law ; when a monochromatic light passes through an absorbing medium the absorbance is directly proportional to the concentration of the medium and the path length
  • 20. 19. Vortex shaker • to mix small amount of liquids quickly in circular motion
  • 21. 20. Semi auto analyser • samples and reagents are mixed and read manually • based on colorimetry principle
  • 22. 21. Water bath • the temp. of water is maintained at a constant level. • boiling point of water - 100°C • it is used to incubate samples over a period of time at a constant temp.
  • 23. 22. Glucose tolerance test curves
  • 24. 23. Plasma protein electrophoresis • A - albumin • B - alpha 1 • C - alpha 2 • D - Beta globulins • E - Gamma globulins
  • 25. • diagnosis - multiple myeloma (cancer of plasma cells) • tumor marker - bence jone's proteins • abnormal band between beta globulins and gamma globulins
  • 26. Benedicts reagent (dark blue) Sulphur powder (light yellow)
  • 27. Biuret reagent (light blue) Picric acid (dark yellow)