Copy of Cellular Morphology for biotech engineering
- 1. Immunological Techniques to Study Cell Psychology Name: Samanta Student ID:23103006 Supervisor: Dr. Malavika Bhattacharya Co-Supervisors: Sudeshna Sengupta, Rojina Khatun
- 2. Abstract Understanding cell psychology involves exploring how cells perceive, respond to, and adapt to various internal and external stimuli. Immunological techniques offer precise and sensitive methods to probe these cellular responses. This study focuses on the use of tools like ELISA, flow cytometry, and immunofluorescence to study cell signaling, surface markers, and protein expression patterns. Through these methods, we aim to unravel how immune-related signaling pathways influence cellular behavior, communication, and decision-making processes. The insights gathered help bridge the gap between immunology and cellular neurobiology, offering new perspectives on disease mechanisms and therapeutic strategies.
- 3. Methodology Techniques Used: Remarks ELISA (Enzyme-Linked Immunosorbent Assay): Quantified cytokine levels to assess immune signaling. Flow Cytometry: Analyzed surface marker expression and cell cycle phases in different cell types. Immunofluorescence Microscopy: Visualized protein localization and cell morphology. Procedure Overview: 1. Cultured immune and neuronal cell lines. 2. Treated with specific stimuli (e.g., cytokines, stress hormones). 3. Samples processed and stained with fluorescent-tagged antibodies. 4. Data analyzed using statistical and image analysis software.
- 4. Findings ELISA Results: Showed elevated IL-6 and TNF-α in response to stress in immune cells. Flow Cytometry: Revealed increased expression of CD86 and MHC-II in activated cells, indicating heightened immune alertness. Immunofluorescence: Detected nuclear translocation of NF-κB, confirming activation of inflammatory pathways. Interpretation: Cells exhibit distinct biochemical signatures in response to psychological stressors, supporting a link between immunity and cellular “decision-making.”
- 5. Conclusion ❖ Immunological techniques effectively reveal the biochemical and structural changes underlying cell responses. ❖ Findings support the concept that cells exhibit complex behavior akin to psychological processing at the molecular level. ❖ This interdisciplinary approach enhances our understanding of how stress and immunity intersect in health and disease. ❖ Future work will explore how chronic immune activation influences neuroplasticity and cell fate decisions.
- 6. References Turner, J. R., & Muller, W. A. (2023). Cortisol, stress, and the immune system. Nature Reviews Immunology, 23(1), 12–24. https://doi.org/10.1038/s41577-022-00795-z Kemeny, M. E. (2003). The psychobiology of stress. Current Directions in Psychological Science, 12(4), 124–129. https://doi.org/10.1111/1467-8721.01246 Nolan, D. A., et al. (2020). Dopamine primes the NLRP3 inflammasome in human monocyte-derived macrophages. Frontiers in Immunology, 11, 663102. https://doi.org/10.3389/fimmu.2020.663102 Zhang, X., Wang, Y., & Li, Y. (2020). Chronic stress and immune function: A review. Journal of Immunology Research, 2020, 1–10. https://doi.org/10.1155/2020/123456 01 03 02 04 05 Irwin, M. R., & Cole, S. W. (2011). Reciprocal regulation of the neural and innate immune systems. Nature Reviews Immunology, 11(9), 625–632. https://doi.org/10.1038/nri3042
- 7. Publication Immunological techniques to study cell psychology Sayani Samanta,Rojina khatun, Sudeshna sengupta, Malavika Bhattacharya. COMMUNICATED
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