cre-lox and cre recombinases in Mouse Genome Informatics (MGI): Module 2
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The document discusses the use of the cre-lox system for targeted gene editing in mice, highlighting the generation of conditional alleles through breeding floxed mice with cre recombinase mice. It details the characterization and discovery of recombinase activities across various tissues and references a database for locating information about targeted alleles. For further inquiries, users are encouraged to contact MGI support.
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cre-lox and cre recombinases in Mouse Genome Informatics (MGI): Module 2
- 1. Module 2. cre-lox and cre recombinases in MGI
- 2. The cre-Lox system 1. mouse with a targeted “floxed” allele 2. mouse with a cre recombinase enzyme expressed under the control of a transgenic promoter Breed together to generate a line that carries both Tissue specific deletion of floxed allele in tissues where cre recombinase is expressed
- 3. Targeted conditional and conditional-ready alleles
- 4. Summary of Brca1 alleles: use category to identify attributes
- 5. Locating information about a targeted allele Reference
- 6. cre recombinase transgenes: by driver or by tissue
- 8. cre recombinases with activity examined in bone marrow
- 11. Systems level recombinase activity detail
- 12. Summary MGI allows users to discover of conditionally targeted alleles and appropriate recombinase Transgene recombinase alleles are characterized across the whole mouse with data presented from multiple sources contact mgi-help@jax.org with any questions