ADO.NET acts as a mediator between client-side and server-side code. It allows applications written in different languages to interact with various backend databases. ADO.NET provides classes like Connection, Command, DataReader, DataAdapter and DataSet to connect to databases, execute queries, retrieve results and display them to users. The Connection class establishes a connection, Command passes queries and operations, DataReader reads query results sequentially, DataAdapter fills DataSet/DataTables with results, and DataSet stores results in memory.