CURCUMIN (Curcuma longa)
- 2. OUTLINE • Synonym • Biological source • Chemical constituents • Isolation • Identification • Analysis • Uses
- 3. SYNONYM: Indian Saffron, Turmeric, Haldi, Haridra BIOLOGICAL SOURCE: Turmeric cosists of dried as well as fresh “Rhizomes” Of the plant Curcuma longa FAMILY: Zingiberaceae COLOUR:Orangeyellowtoyellow ODOUR:Aromatic TASTE:Warmlyaromatic&Bitter ORGANOLEPTICCHARACTERS
- 4. PLANTHABITAT • Turmericisaperennialherbaceousplant • Theleavesarealternateandarrangedintworows CHEMICALCONSTITUENTS Majorconstituents:Curcumin 50-60%Essentialoil2-7%withhighcontentbisabolane derivatives ItalsocontainsDesmethoxycurcumin(DMC), Bisdesmethoxycurcumin(BDMC), commonphytosterols,ar-tumerone,Zingiberenefattyacidsandpolysaccharides.
- 5. ISOLATION OF CURCUMIN Curcumin can be obtained by different process 1. Turmeric powder is extracted with alcohol in soxhlet extractor The alcoholic extract is concentrated under reduced pressure and dried
- 6. 2. In another procedure, turmeric powder is first extracted with hexane followed by Acetone The acetone extract is concentrated with hexane followed by acetone The acetone extract is concentrated & dried to yield CURCUMIN
- 7. IDENTIFICATION TESTS Chemical Test: • Powered drug + sulphuric acid gives Crimson colour • The aqueous solution of turmeric + boric acid gives reddish brown colour which on addition of alkali changes to greenish blue TLC method Sample preparation : Dissolve 1mg of curcumin in 1mL of methanol Stationary phase : Silica gel G Mobile phase : Chloroform:Ethanol:Glacial acetic acid (94:5:1) Detecting Agent : Anisaldehyde sulphuric acid reagent Rf valve : 0.79,0.60 and 0.43 Colour Spot : Bright yellow fluorescent
- 8. ANALYSIS OF CURCUMIN METHODS : • By spectrophotometeric Method • Estimation by HPLC • Estimation by Alkalimetric Method ESTIMATION BY HPLC Column : 5um Nucleosil NH2 (Chromapack) Mobile Phase : Etahnol Flow rate : 1.2mL/min Detection : UV-254nm Peak retention time: Bisdemethoxy curcumin 4.5-5 minutes Demethoxy curcumin 6-7 minutes Curcumin 11-12 minutes