CV_ABUL_2015
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This document provides a summary of Abul Hasan Sardar's education, research experience, publications, and skills. It summarizes that he has a PhD in Microbiology from the University of Calcutta, has worked as a Senior Research Fellow studying Leishmania donovani at Rajendra Memorial Research Institute of Medical Sciences since 2010, and has authored several publications on proteomic analyses of L. donovani stress responses and drug resistance mechanisms.
CV_ABUL_2015
- 1. Abul Hasan Sardar Division of Molecular Biology, Rajendra Memorial Research Institute of Medical Sciences, Affiliated with University of Calcutta, Kolkata Kolkata, WB 700019, India Mobile- 91-8820122772, 91-9475055633 Email-abulhasansardar82@gmail.com EDUCATION University of Calcutta, Department of Microbiology, Kolkata, India Doctor of Philosophy, Microbiology; Emphasis: Quantitative Proteomics, Mechanism of Stress Management, December’ 2014 (Thesis submitted) The Global Open University, Nagaland, India Master of Philosophy, Biochemistry; Emphasis: Differential Proteomics, Drug Resistance, March 2010 University of Kalyani, Kalyani, India Master of Science, Microbiology, December 2004 University of Calcutta, Kolkata, India Bachelor of Science, Microbiology, January 2002 RESEARH EXPERIENCE Rajendra Memorial Research Institute of Medical Sciences, India Senior Research Fellow 2010 – Present Supervisor: Dr. Pradeep Das Contributed to and led multiple research programs like identification of differentially expressed proteins of Leishmania donovani (causative agent of visceral leishmaniasis) under exposure to different stress environments using iTRAQ reagents and subsequent mass spectroscopy, study the mechanism of stress management, functional characterization of two proteins i.e. argininosuccinate synthase and peroxiredoxin by generating knock-out L. donovani strain, studies on T-cell mediated response of PBMC samples isolated from visceral leishmaniasis (VL) patients to develop DNA vaccine against VL (under LEISHDNAVAX project funded by European Commission). McGill University, Institute of Parasitology, Macdonald campus, Canada Graduate Research Trainee 15th November’2011 to 15th May’2012 Supervisor: Prof. Armando Jardim Trained regarding generation of knock-out strain of L. donovani for specific gene by means of homologous recombination and subsequent transfection. Contributed in developing a novel antigen based detection method for early diagnosis of VL Patient. Rajendra Memorial Research Institute of Medical Sciences, India Research Assistant 2008-2010 Supervisor: Dr. Pradeep Das Contributed in the study for evaluation of rapid diagnostic test for detection visceral leishmaniasis and in the study of anti-leishmanial activity of antimicrobial peptide from sand-fly origin.
- 2. TEACHING EXPERIENCE Sammilani Mahavidyalaya affiliated with University of Calcutta, Kolkata, India Part-Time Lecturer January’2008 to March’2008 Responsibilities: Teaching the undergraduate course in Microbiology. JOB EXPERIENCE Begampur Laboratory, Public Health Engineering Department, Govt. of West Bengal, Kolkata. Bacteriologist 2006-2008 Responsibilities: Routine microbiological and chemical analysis of water sample for drinking purpose. PUBLICATIONS ARTICLES PUBLISHED 1. A. H. Sardar, S. Kumar, A. Kumar, B. Purkait, S. Das, A. Sen, M. Kumar, K. K. Sinha, D. Singh, A. Equbal, V. Ali, P. Das. Proteome changes associated with Leishmania donovani adaptation to oxidative and nitrosative stress. Journal of Proteomics. 81 (2013) 185-189. 2. A. H. Sardar, S. Das, S. Agnihorti, M. Kumar, A. K. Ghosh, K. Abhishek, A. Kumar, B. Purkait, M.Y. Ansari, P. Das. Spinigerin induces apoptotic like cell death in a caspase independent manner in Leishmania donovani. Experimental Parasitology. 135 (2013) 715–725. 3. B. Purkait, A. Kumar, N. Nandi, A. H. Sardar, S. Das, S. Kumar, K. Pandey, V. N. Ravidas, M. Kumar, T. De, D. Singh and P. Das. Mechanism of Amphotericin B resistance in clinical isolates of Leishmania donovani. Antimicrobial Agents and Chemotherapy. 56 (2) (2012) 1031-41. 4. A. Kumar, S. Das, B. Purkait, A. H. Sardar, A. K. Ghosh, M. R. Dikshit, K. Abhishek. Ascorbate Peroxidase: a key molecule regulating the Amphotericin B resistance in clinical isolates of Leishmania donovani. Antimicrobial Agents and Chemotherapy. 58(10) (2014) 6172-6184. 5. S. Das, K. Pandey, A. Kumar, A. H. Sardar, B. Purkait, M. Kumar, S. Kumar , V. N. Ravidas, S. Roy, D. Singh and P. Das. TGF-β1 re-programs TLR4 signaling in L. donovani infection: enhancement of SHP-1 and ubiquitin- editing enzyme A20. Immunology and Cell Biology. 90 (2012) 640-654. 6. B. Purkait, R. Sing, K. Wasnik, S. Das, A. Kumar, M. Paine, M. R. Dikshit, A. H. Sardar, A. K. Ghosh and P. Das. Up-regulation of Silent Information Regulator 2 is associated with Amphotericin B resistance in clinical isolates of Leishmania donovani. Journal of Antimicrobial Chemotherapy (Accepted on 30th November’2014). 7. A. Equbal, S. S. Suman, S. Anwar, K. P. Singh, A. Zaidi, A. H. Sardar, P. Das and Vahab Ali. Stage-Dependent Expression and Up-Regulation of Trypanothione Synthetase in Amphotericin B Resistant Leishmania donovani”. PLOS ONE, 2014. 8. S. Kumar, R. Banerjee, N. Nandi, A. H. Sardar and P. Das. Anokis Potential of Entameba histolytica Secretary Proteins Cysteine proteases: Evidence of Contact independent Host Cell Death. Microbial Pathogenesis. 52 (1) (2012) 69-76.
- 3. 9. D. Singh, K. Pandey, V. N. R. Das, S. Das, N. Verma, A. Ranjan, S. C. Lal, R. K. Topno, S. K. Singh, R. B. Verma, A. Kumar, A. H. Sardar, B. Purkait and P. Das. Evaluation of rk-39 strip test using urine for diagnosis of Visceral Leishmaniasis in an endemic region of India. Am. J. Trop. Med. Hyg. 88(2) (2013) 222-226. ABSRACTS PUBLISHED IN PROCEEINGS/SYMPOSIA 1. A. H. Sardar, S. Kumar, A. Jardim, P. Das et. al. “iTRAQ chemistry explore the proteome changes associated Leishmania donovani promastigote adaptation to oxidative and nitrosative stress at the 81st Annual Symposium of Society for Biological Chemist (SBC-2012), 8-11 Nov’2012, Kolkata, India. 2. A. H. Sardar, S. Kumar, A. Kumar, A. Jardim, P Das et. al. “Proteome changes associated Leishmania donovani promastigote adaptation to oxidative and nitrosative stress” at the 4th Annual Symposium of Canadian National Proteomics Network, April 23-25, 2012, Toronto, Canada. 3. A. Kumar, A.H. Saradar, M.R. Dikshit, Singh, P. Das et. al. “Ascorbate peroxidase over expression protects drug resistance L. donovani cells against reactive oxygen species mediated auto-oxidation of Amphotericin-B” at the Antimicrobial Resistance conference, 6th -8th Feb’2012, Allahabad, India. 4. B. Purkait, A. Kumar, A. H. Sardar, S. Das, P. Das et. al. “Amphotericin-B resistance mechanism in clinical Isolates of L. donovani” at the Antimicrobial Resistance conference, 6th -8th Feb’2012, Allahabad, India. 5. A. H. Sardar, S. Kumar, K. K. Sinha, D. Singh, P. Das et. al. “Proteome profiling explores metabolic changes to adapt Leishmania donovani on exposure to oxidative and nitrosative stress” at the 4th Bihar Vigyan Congress at Indira Gandhi Planetarium, 2011, Patna, India. 6. S. Agnihorti & A. H. Sardar, S. Das, D. Singh and P. Das et. al., “The cationic AMP spinigerin induces apoptotic like cell death in a caspase independent manner in Leishmania donovani” at the 4th Bihar Vigyan Congress at Indira Gandhi Planetarium, 2011, Patna, India. 7. Sen, A. Kumar, S. Das, A. H. Sardar, B. Purkait, N. Nandi and P. Das “20S Proteasome beta subunit and Amastin are important factors for survival and pathogenesis of leishmaniasis: A DNA microarray study” at Tropacon’ 16-19 Nov’2009 at PGIMER, Chandigarh, India. 8. B. Purkait, N. Nandi, A. Kumar, A. H. Sardar, S. Kumar, P. Das. “Analysis of the involvement of thiol metabolic pathway in Amb resistant mechanism for Leishmania donovani.” At the 4th Workshop of the Seattle-India Joint Research Training Programme “Research Training on Intracellular Pathogens” 2-7 Feb’ 2010, Goa. MANUSCRIPT UNDER REVISION 1. A. K. Ghosh, A. H. Sardar, A. Mandal, S. Saini, K. Abhishek, A. Kumar, B. Purkait, R. Singh, S. Das, R. Mukhopadhyay, S. Roy and P. Das. Metabolic reconfiguration of the central glucose metabolism: a crucial strategy of Leishmania donovani for its survival during oxidative stress”, The FASEB journal (major revision). MANUSCRIPT UNDER CONSTRUCTION 2. A. H. Sardar, A. Jardim, A. K. Ghosh, A. Mandal , S. Das, S. Saini, K. Abhishek, B. Purkait and P. Das. Genetic manipulation of Leishmania donovani to explore the involvement of argininosuccinate synthase in oxidative stress management. To be communicated in PLOS PATHOGENS.
- 4. Gene Sequences Submitted in NCBI 1. Leishmania donovani oleate desaturase mRNA, partial cds, , Kumar, M., Sinha, K., Sardar, A. H. and Das, P. (ACCESSION HM454284). 2. Leishmania donovani NAD-dependent histone deacetylase silent information regulator 2 (Sir2RP) mRNA, complete cds. Purkait, B. Kumar, A. Sardar, A. H. Das, S. Kumar, M. and Das,P. (ACCESSION JN621326), 3. Leishmania donovani thioredoxin mRNA, complete cds., Purkait, B. Kumar, A. Sardar, A. H. Das, S. Kumar, M. and Das,P. (ACCESSION JQ796816). 4. Leishmania donovani ascorbate peroxidase (APx) mRNA, complete cds., Kumar, A. Purkait, B. Sardar, A. H. Das, S. Kumar, M. and Das, P. (ACCESSION JN802370). SYMPOSIA ATTENDED 1. 81st Annual Symposium of Society for Biological Chemist (SBC-2012), 8-11 Nov’2012, Kolkata, India. 2. 4th Annual Symposium of Canadian National Proteomics Network, April 23-25, 2012, Toronto, Canada. 3. 4th Bihar Vigyan Congress at Indira Gandhi Planetarium, 2011, Patna, India. TECHNICAL SKILLS Molecular Biologist with a strong working background in quantitative proteomics, genomic techniques, cell biology and microscopy. Biochemical: Protein isolation and purification, 2-D Gel Electrophoresis, Quantitative proteomics (Mass Spectroscopy, large scale proteome data analysis), Enzyme assay, HPLC, FPLC, SDS and native polyacrylamide gel electrophoresis. Spectroscopic: Ultraviolet, fluorescence, visible absorption; Spectrofulorometry. Microscopy: Fluorescence microscopy; Phase contrast microscopy. Molecular Biology: Microarray; Vector construction and cloning of gene; Isolation DNA and RNA from protozoa and other types of cells; Plasmid DNA isolation; Transformation of bacteria; Expression of recombinant protein; Site-directed mutagenesis, generation of knock-out strains,Western blot; Northern Blot; Southern Blot; PCR; Semiquantitative PCR; Realtime PCR; DNA sequencing using automated DNA sequencer; Transfection by electroporation in Bacteria and Protozoa; ELISA, ELISPOT and other commonly used microbial techniques.
- 5. AWARDS/HONORS McGill University, Institute of Parasitology, Macdonald campus, Canada Canadian Commonwealth Fellow 2011-2012 Canadian National Proteomics Travel Award 2012 Rajendra Memorial Research Institute of Medical Sciences, India Senior Research Fellow (Intramural Project, funded by Indian Council of Medical Research) 2012-2014 Senior Research Fellow (LEISHDNAVAX project, Funded by European Commission) 2010-2012 REFEREES Dr. Pradeep Das Dr. Armando Jardim Dr. Kislay Kumar Sinha Dr. Samir Kumar Mukherjee (PhD Supervisor) Associate Professor Asst. Prof. Biotechnology Associate Professor Director Director, Centre for National Institute of Department of Microbiology Rajendra Memorial Research Host-Pathogen Interactions Pharmaceutical Education University of Kalyani Institutes of Medical Sciences Institute of Parasitology and Research Kalyani-741235, Agamkuan, Patna-800007, McGill University EPIP, Jalandhar Road, Hajipur Tel-033-25827315 (ext-332) Mobile-91-9431012380 21 111, Lakeshore Road Vaishali-844101, India Fax-033-2582-8282 drpradeep.das@gmail.com QC H9X3V9 Mobile No:+91-9279133837 Mobile-91-9433136617 Tel: (514)398-7727 kksinha@yahoo.com dr.samirmukherjee@gmail.com Fax: (514) 398-7857 armando.jardim@mcgill.ca