Detection of cellulose in milk
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The document discusses the detection of cellulose in milk using an iodine-zinc chloride reagent, which produces a blue color indicating its presence. Cellulose is a polysaccharide found in plant cell walls, and its content varies across different materials, with cotton fiber being the purest source. The document also details a laboratory procedure for isolating cellulose from milk and its various applications in industrial use.
Detection of cellulose in milk
- 1. Detection of Cellulose in Milk F a r h a n g H a j i H m a i d I R A Q - K u r d i s t a n - s u l a y m a n i y a h + 9 6 4 7 7 0 1 4 2 0 9 6 2 F a r h a n g p d k . h a m i d @ g m a i l . c o m 5 / 2 0 / 2 0 1 6 Farhang Hamid Cellulose isalongchainof linkedsugarmoleculesthatgiveswoodits remarkable strength.Itisthe maincomponentof plantcell walls,and the basic buildingblockformanytextiles andforpaper.Cottonisthe purestnatural formof cellulose.Inthe laboratory, ashless filterpaperis a source of nearlypure cellulose.
- 2. Detection of Cellulose in Milk حليب في سليولوز تقدير Cellulose in milk gives blue color with Iodine – Zinc Chloride reagent. Cellulose is an organic compound with the formula (C6H10O5) n, a polysaccharide consisting of a linear chain of several hundred to many thousands of β(1→4) linked D-glucose units Cellulose is an important structural component of the primary cell wall of green plants, many forms of algae and the oomycetes. Some species of bacteria secrete it to form biofilms. Cellulose is the most abundant organic polymer on Earth. The cellulose content of cotton fiber is 90%, that of wood is 40–50% and that of dried hemp is approximately 57%. Cellulose is mainly used to produce paperboard and paper. Smaller quantities are converted into a wide variety of derivative products such as cellophane and rayon. Conversion of cellulose from energy crops into biofuels such as cellulosic ethanol is under investigation as an alternative fuel source. Cellulose for industrial use is mainly obtained from wood pulp and cotton. Some animals, particularly ruminants and termites, can digest cellulose with the help of symbiotic micro-organisms that live in their guts, such as Tri chonympha. In humans, cellulose acts as a hydrophilic bulking agent for feces and is often referred to as a "dietary fiber". Reagent: Iodine-Zinc Chloride reagent: Dissolve 20.0 gm ZnCl2 in 8.5 ml water and when cool, introduce the iodine solution (3.0 gm potassium iodide and 1.5 gm iodine in 60 ml water) drop by drop until iodine begins to precipitate. Procedure: -1 Take about 10.0 gm of milk in a 100 ml beaker. -2 Add 50 ml of hot water and stirred thoroughly for about 2 minutes. -3 Pour the mixture on a nylon cloth and wash the residue with 50 ml of hot water twice. -4 Scrape the residue with a spatula and place it in a spotting plate. -5 Stain a part of residue with Iodine-Zinc Chloride reagent and another part with iodine solution. -6 Development of blue color in Iodine-Zinc Chloride reagent and absence of blue color in Iodine Solution confirms presence of cellulose. Note: The method is also applicable to milk products like curd, rabri and evaporated milk.