Development and commercialization for bio pesticide by using pseudomonas

DEVELOPMENT AND
COMMERCIALIZATION FOR BIO-
PESTICIDE BY USING
PSEUDOMONAS FLUORESCENCE

INTRODUCTION
 P. fluorescence is gram negative bacillus
 These bacteria are easy to culture, as they grow on
minimal media and at 25-30 degrees Celsius
 They destroy certain toxins and pollutants including
styrene, polycyclic aromatic hydrocarbons, and TNT
 They also protect plants from infection by pathogens by
producing secondary metabolites like antibiotics and
hydrogen cyanide which kill other bacteria and fungi

ABSTRACT
 In physical characteristic test in various media
P. fluorescence shows positive growth
 In biochemical characteristic on various media
P. fluorescence shows positive result
 In anti-pathogenic activity test of P. fluorescence also
shows positive result against F. oxysporum. This test
confirm that P. fluorescence can be use as bio-fertilizer

MATERIAL AND METHOD
 All the pure culture of P. fluorescence are taken from DESIGN
BIOSIS
 All chemical & media were prepared to evaluate the
biochemical assay, physical appearance & anti-pathogenic
activity test of P. fluorescence
 Also microscopic observation of P. fluorescence was done

Physical activity test
various media was used to determine the physical appearance of
P. fluorescence
Those are
 King’s B agar media
 King’s Broth media
 Starch agar and starch broth media
 LB Broth media
 pikovaskaya's agar and broth media

Biochemical activity test
various media was use to determine the Biochemical activity test
for P. fluorescence
Those are
 Citrate agar slant media
 Starch agar media
 Czapek agar medium

Anti-pathogenic activity test
various media was use to determine the anti-pathogenic activity
test for P. fluorescence
Those are
 LB agar media
 Potato dextrose agar medium

Physical characteristic photos
King’s B agar medium

King’s B Broth media LB Broth media

Starch Broth media Starch agar media

Pikovaskaya's agar media Pikovaskaya's agar media

Biochemical activity test photos
Citrate agar medium

In PDA media F.oxysporum
P. fluorescence
Mat based inhibition of F.oxysporium(Plant
Pathogen)

In PDA media
F. oxysporum P. fluorescenceF.oxysporum
Inoculum based inhibition of F.oxysporium
(Plant -Pathogen)

In LB agar media
F. oxysporum
P. fluorescence
F. oxysporium is inoculated in middle and P. fluorescence Inoculated
in around that F. oxysporium(Plant pathogen)

In LB agar media
F. oxysporum
P. fluorescence
Mat based inhibition of F.oxysporum (Plant Pathogen)

Mass Culture, Bio-formulation and
commercialization
Blander

Mass culture of
Pseudomonas
fluorescence
Market product

RESULT
Physical activity test
 From the above photos we can observed that in starch
agar media the growth of Pseudomonas fluorescence
is greater than Kings B media, pikovaskaya's media.
 In fact in pikovaskaya's agar slant media was to less.

Biochemical activity test
 The biochemical assay confirmed that Pseudomonas
fluorescence has citrate utilizing, starch hydrolysis and
cellulose activity.
 This experiment shows the proof and practical identification
and confirmation method for P. fluorescence.

 The anti-pathogenic activity of P. fluorescence against F.
oxysporum shows that the mat based formulation of bio-
fertilizer is effective against F. oxysporum rather than liquid
based preparation.
 Also P. fluorescence can be added in combination with other
soil friendly microorganisms to inhibit of the plant pathogens
including F. oxysporum in it’s soil based application

Mass Culture, Bio-formulation and
commercialization
 The bio-formulation of P. fluorescence is an easy way if can be
done by P. fluorescence Mat mixing with Talc powder and
CMC.
 The commercialization of this bio-formulation can be widely
used as the plant pathogen eradication or control by using
natural resources are very few in Agriculture sector.

REFERENCE
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Development and commercialization for bio pesticide by using pseudomonas

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