Development of inoculum ppt
- 2. Synapsis :- Introduction Definition of Inoculum Constituent of Inoculum media Steps Involving Inoculum development Examples of Inoculum media used in various Industrial Production Conclusion References
- 3. Introduction :- Inoculum production is a critical stage in an Industrial fermentation process. Obviously, one loop of celline requires a prolonged period if it is directly introduced in to fermentation. Thus, inoculum is prepared as a stepwise sequence employing increasing volumes of media.
- 4. Definition of inoculum :- Inoculum is the mixture of cultured microbes Along with media in which it is growing.
- 5. Constituent of Inoculum media :- Chemical composition :- The Inoculum media must have a suitable Chemical composition. Generally, the medium should contain a source of carbon, a source of Nitrogen, growth factors and Mineral salts. Buffering Capacity :- Maintenance of the PH in the optimum range is necessary for making the process successful. In order the control the PH of the medium, buffers (e.g. CaCo ) Should be added to the medium.
- 6. Avoidance of foaming :- 1.Foaming is a serious problem in a fermentation Industry. 2.Hence, defoamers (e.g. oil mixed with Octadecanol for penicillin fermentations) should be used for controlling foam. Consistency :- 1. Proper airation and agitation.
- 7. Examples of Inoculum media used in various Industrial production :- 1. Compostions of Bennett’s medium used in the prepartion of Inoculum for vitamin productions :- Component Amount (g./Litre) Yeast extract Beef extract N-Z-Amine A Glucose Distilled water 1.0 gm 1.0 gm 2.0 gm 10.0 gm 15.0 ml.