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Montana State University
Pestalotiopsis in the World’s Rainforests
Endophytes Identification: morphological methods
Pestalotiopsis microspora an Endophytic Fungus
1. One of the most frequently observed
   endophytes.
2. Found in all rainforests and most plant
  species.
3. It has hundreds of synonyms.
4. Exists as many biotypes and makes hundreds
  of natural products.
5. Some biotypes are easily genetically
  transformed.
Pestalotiopsis microspora (culture and
                spores)
The Acervulus and Conidia of
 Pestalotiopsis microspora
Pestalosphaeria hansenii on Barley
The Perithecium and Ascopsores of
Pestalosphaeria hansensii, Perfect Stage of
Pestalotiopsis microspora
Endophytes Identification: morphological methods
Endophytes Identification: morphological methods
Picking the Spines of Cholla Cactus
To Support the Bioassay Chemicals
Developing a Bioassay for Sex
Inducing Chemicals found in Plants
Endophytes Identification: morphological methods
Ability of Various Fractions of T cuspidata to
Induce the formation of fruitng structures
The Induced Perithecia of Pestalosphaeria
Endophytes Identification: morphological methods
Endophytes Identification: morphological methods
Protoplasts of P. microspora Ready
    for Genetic Transformation
Selection of Hygromycin Resistant Isolates
Endophytes Identification: morphological methods
Endophytes Identification: morphological methods
Endophytes Identification: morphological methods
Endophytes Identification: morphological methods
Methylene chloride extracts
of P. microspora isolates from
       around the world
Sepik River Area of Papua New Guinea
Sepik River Area of Papua New Guinea
Crystals of Pestacin
Pestacin-a
Powerful
Antioxidant
Isopestacin- an
antioxidant
Sampling trees for Endophytic
Fungi in the Highlands of Papua
New Guinea
Pestalotiopsis jesterii, a fungal endophyte
Conidiospores of P. jesterii
Endophytes Identification: morphological methods
Endophytes Identification: morphological methods
Endophytes Identification: morphological methods
Test Fungus       Natural   Synthetic
                    MIC       MIC

P. Ultimum            25        25

R. Solani             25        50


G. Candidum          100        100

P. oryzae             25        25


S. sclerotiorum      100        100
Huli Wigmen of Papua New Guinea
Pestalotiopsis
microspora as
an endophytic
rainforest
fungus has
made the “big
time” as a
producer of
new natural
products
The Orchid -Dendrochilum sp.
Solid State NMR of Ambuic Acid
Ambuic Acid is
produced by
various isolates of
Pestalotiopsis
microspora
obtained in various
rainforest
locations around
the world
Torreya taxifolia and torreyanic acid
Torreyanic acid
Anticancer
agent
Pestaloside

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Endophytes Identification: morphological methods