Field portable GC/MS - Technology and applications
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The document presents field-portable GC/MS technology, emphasizing ease of use, speed, and analytical capabilities for applications in food safety and quality. It details the components, processes, and methodologies, including solid phase microextraction and specific performance metrics. Key takeaways highlight its applicability in both on-site and laboratory settings for analyzing freshness, flavor, authenticity, and contaminants.
Field portable GC/MS - Technology and applications
- 1. Field-Portable GC/MS Technology & applications Dr. Joeri Vercammen Managing Expert IS-X www.is-x.com
- 3. Triple quadrupole GC/MSMS Multipurpose autosampler Ultratrace analysis Runtime: 45 minutes Expert knowledge required
- 4. RIVM “MOBILE CHEMICAL & BACTERIOLOGICAL LABORATORY”
- 5. Dimensions: 38 cm x 39 cm x 23 cm Weight: < 14.5 kg Power: LiH battery Carrier gas: Helium cartridge
- 6. Sample prep/extraction GC separation MS detection Data processing 1 3 2 4
- 7. 3 2 4 Sample prep/extraction GC separation MS detection Data processing
- 8. Solid Phase Microextraction (SPME) Fiber coated with polymeric material Expose to sample Enrich components Desorb in GC injection port
- 9. Solid Phase Microextraction (SPME) First described in 1990 by J. Pawliszyn (Waterloo, Canada) # 10.482 search results “SPME” in ScienceDirect # 7.585 search results “SPME + food” 0 100 200 300 400 500 600 700 800 900 1000 1995 1997 1999 2001 2003 2005 2007 2009 2011 2013 2015 #Publications Year
- 10. CUSTODION-SPME “More robust configuration of the classic SPME technique”
- 11. CUSTODION-NT “Triple bed needle trap for dynamic sampling at low concentration”
- 12. CUSTODION-ADS “Ideal for direct sampling of volatiles from surfaces”
- 13. 1 3 4 Sample prep/extraction GC separation MS detection Data processing
- 14. Technology: Low thermal mass GC Column: MXT-5, 5 m x 0.1 mm x 0.4 µm Carrier: Helium @ 25 psig (EPC) Injection: S/SL with Merlin head Oven: 50°C (10 s) to 300°C (10 s) @ 2°C/s R2R time: 5.00 min
- 15. 1 2 4 Sample prep/extraction GC separation MS detection Data processing
- 16. Diameter: 4 mm Trap heater: 150-180°C Vacuum: 2-stage turbo + diaphragm Ionisation: 70 eV Mass range: 45 – 500 Da Scan rate: 10 scans/s
- 17. 1 3 2 Sample prep/extraction GC separation MS detection Data processing
- 18. Each datafile is processed automatically with the software. The algorithm is proprietary and identifies peaks based on spectral matching & retention index. DATA PROCESSING
- 19. START UP PROCEDURE Switch instrument ON Wait for idle (2.00 minutes) Blank analysis: pass/fail? Inject QC standard: pass/fail? Start analyses
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- 23. APPLICATIONS Shelflife & freshness Flavour & fragrance Authenticity Packaging Toxins Pesticides …
- 26. In vivo SPME with in vitro calibration: Determination of off-flavor components in live fish Z. Bai e.a., Anal. Chem. (1993) ,
- 27. 1 Strawberries
- 29. 2.00 min
- 31. Butanoic acid, ethyl ester (86%)
- 32. Hexanoic acid, ethyl ester (77%)
- 33. 0.0E+00 5.0E+09 1.0E+10 1.5E+10 2.0E+10 2.5E+10 0 2 4 6 8 10 12 14 Time (Days) Butanoic acid, ethyl ester Butanoic acid, 2-methyl-, ethyl ester 1-Butanol, 3-methyl-, acetate Hexanoic acid, ethyl ester Acetic acid, hexyl ester Benzoic acid, ethyl ester Ethyl acetate Propanoic acid, 2-methyl-, ethyl ester
- 34. 0.0E+00 5.0E+09 1.0E+10 1.5E+10 2.0E+10 2.5E+10 0 2 4 6 8 10 12 14 Time (Days) Butanoic acid, ethyl ester Butanoic acid, 2-methyl-, ethyl ester 1-Butanol, 3-methyl-, acetate Hexanoic acid, ethyl ester Acetic acid, hexyl ester Benzoic acid, ethyl ester Ethyl acetate Propanoic acid, 2-methyl-, ethyl ester
- 35. 2 Chicken Filet
- 36. 0.0E+00 5.0E+08 1.0E+09 1.5E+09 2.0E+09 2.5E+09 3.0E+09 3.5E+09 4.0E+09 4.5E+09 0 1 2 3 4 5 6 7 8 9 Time (Days) >Disulfide, dimethyl >Dimethyl trisulfide >Ethanol, 2-(2-ethoxyethoxy)- >Benzene, 2,4-diisocyanato-1-methyl- >2H-Benzimidazol-2-one, 1,3-dihydro-5-methyl- >Pyridine, 4-(1-pyrrolidinyl)-
- 37. 0.0E+00 5.0E+08 1.0E+09 1.5E+09 2.0E+09 2.5E+09 3.0E+09 3.5E+09 4.0E+09 4.5E+09 0 1 2 3 4 5 6 7 8 9 Time (Days) >Disulfide, dimethyl >Dimethyl trisulfide >Ethanol, 2-(2-ethoxyethoxy)- >Benzene, 2,4-diisocyanato-1-methyl- >2H-Benzimidazol-2-one, 1,3-dihydro-5-methyl- >Pyridine, 4-(1-pyrrolidinyl)-
- 38. 3 Pistachio Nuts
- 39. 0.0E+00 1.0E+08 2.0E+08 3.0E+08 4.0E+08 5.0E+08 6.0E+08 7.0E+08 8.0E+08 9.0E+08 0 2 4 6 8 10 12 14 Time (Days) Butanal, 3-methyl- Butanal, 2-methyl- Hexanal Ethanol, 2-(2-ethoxyethoxy)- 3-Carene
- 40. 0.0E+00 1.0E+08 2.0E+08 3.0E+08 4.0E+08 5.0E+08 6.0E+08 7.0E+08 8.0E+08 9.0E+08 0 2 4 6 8 10 12 14 Time (Days) Butanal, 3-methyl- Butanal, 2-methyl- Hexanal Ethanol, 2-(2-ethoxyethoxy)- 3-Carene
- 41. KEY TAKE AWAYS Easy-to-use field portable GC/MS system with immediate applicability in food-related analysis. Both ons-site as in the lab!
Editor's Notes
- #13: Accelerated diffusion sampler