HDT Practical Exp. 7
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This experiment aimed to determine the volume of citral present in a sample of lemongrass oil. Lemongrass oil contains at least 75% aldehyde, including citral, and is used in perfumes and flavorings. The procedure involved adding lemongrass oil to a sodium sulfite solution, heating to allow citral to react and form sodium citrate, then neutralizing and continuing to heat until the solution turned clear, indicating the reaction was complete. The separated oil volume would then indicate the original citral content in the sample. The report would state the exact citral volume determined from this experiment.
HDT Practical Exp. 7
- 1. EXPERIMENT: 7 DETERMINATION OF ALDEHYDE CONTENT By Mrs. Poonam N. Chougule Assistant Professor and HOD of Pharmacognosy department AMCP, Peth- Vadgaon.
- 2. Aim: To determine the volume of Citral present in the given sample Lemon grass oil. Requirement: 5% of Sodium sulphite, lemongrass oil, Phenolphthalein, 30% acetic acid, Cassia flask. Principle: Lemon grass oil is a volatile oil by steam distillation of leaves and aerial parts of Cymbopogon flexous, graminae. It contains NLT 75% of aldehyde, is used as a flavouring agent in perfumery industries and employed as a source of citral from which beta ionone is prepared is prepared which is a starting material for the synthesis of Vitamin A. Citral reacts with sodium sulphide to form monomer sodium sulphide due to the presence of conjugated aldehyde group in citral. Acetic acid is used to neutralize the alkali medium which are formed by heating. The
- 3. Procedure- Take 70 ml of 5% solution of sodium sulphite in conical flask. Add 10ml of lemon grass oil into it and then add sufficient amount of phenolphthalein gives distinct pink color. Heat this mixture in a water bath with shaking. Then, neutralize the 30% acetic acid and continue the heating for 30-35 min. Also add acetic acid and continue until the pink color fails to return. Add sodium sulphide up to the mark and then undissolved oil separates as a layer and measure the volume.
- 4. Report- The amount of citral present in the given sample of lemon grass oil was found to be ----- ----. Thank You….