Honolulu cloning technique
- 1. Honolulu Cloning Technique By: Ashley Boggs
- 2. What is the Honolulu Cloning Technique? A technique that uses adult cells and nuclei for cloning Used to clone three generations of mice First clone was known as Cumulina
- 3. Honolulu Cloning First used in July of 1998 Experiment was at the University of Hawaii Developed by Ryuzo Yanagimachi and Teruhiko Wakayama
- 4. Steps of the Honolulu Technique The cell’s nucleus is removed A donor nucleus is injected into an egg The new cell is chemically cultured to jumpstart cell growth
- 5. Steps of the Honolulu Technique Continued 4. This created embryos 5. Which are placed into the surrogate mothers to be carried The method is repeated over and over to create 2 nd and 3 rd generations of identical mice
- 6. Pros of the Technique Used to create clones, exact copies; genetic replicas Had very successful nuclei used, cumulus cells More successful cloning rate than the Roslin technique Can help scientists do further research on egg reprogramming in a nucleus
- 7. Cons of Cloning It was a process of trial and error, so before there were good clones there were many mistakes and mutations made Reduces genetic diversity Expensive One virus could knock out an entire species of clones because of a lack of genetic diversity
- 8. Uses of Honolulu Cloning To further cell research To produce transgenic animals To produce drugs from transgenic animals Use clones to “evaluate molecular mechanisms” Critique current techniques and create better ones
- 9. The End.