(June 10th, 2021) Learn More About Live Cell Imaging and Analysis
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The document reviews Cytosmart's live cell imaging systems: Lux2, Lux3 FL, and Omni, highlighting their advantages for non-invasive, continuous monitoring of cell cultures. Key features include compact designs, cloud-based image analysis, and compatibility with various culture vessels, facilitating efficient and effective cellular research. Each system offers unique specifications and applications, suitable for tracking cell growth, migration, and other biological processes.
(June 10th, 2021) Learn More About Live Cell Imaging and Analysis
- 1. A CytoSMART product review: The Lux2, Lux3 FL and OMNI Live Cell Imaging Systems Katie Parkins, PhD Applications Scientist
- 2. WWW.SCINTICA.COM Topics of discussion 2 What is live cell imaging? Comparing the options: Lux2, Lux3 FL, OMNI Potential applications What are the advantages?
- 3. WWW.SCINTICA.COM What is live cell imaging? • Cells need to be monitored for cell confluency, cell number, among other things in order to study the fundamentals of cell growth dynamics. • Opening the door of the incubator and removing cells to take measurements and images prevent accurate measurements under physiological conditions • Live cell culture monitoring can be done with compact brightfield microscopes that fit within incubators • Basic brightfield and fluorescent imaging • Routine cell culture processes i.e. tracking cell confluency 3
- 4. WWW.SCINTICA.COM What are the advantages? • Less time consuming- more time for other experiments • Enables noninvasive (no dyes needed) longitudinal studies rather than just “snapshots” • No need for cells to undergo temperature shock by removing cells from incubator • Monitor your cells anytime and anywhere • Less training, personnel, and variability 4
- 5. WWW.SCINTICA.COM System Components • Hand-held design • Compact design • Fits inside incubator • Microscope • Fluorescent Brightfield live cell imaging • User friendly • Compatible with wide range of culture vessels • Software • CytoSMART Cloud • Integrated image analysis (FL + BF) • Notifications when cells are ready 5
- 6. WWW.SCINTICA.COM The CytoSMART product line 6 Lux2 Lux3 FL OMNI
- 8. WWW.SCINTICA.COM Lux2: Technical Specifications 8 Specifications Lux2 System dimensions 133 x 90 x 100 mm Weight 0.5 kg (1.1 lb) Optics Brightfield with digital contrast Magnification 10x fixed objective, digital zoom to 20x Light source LED Camera 5MP CMOS Image size 1280 x 720 pixels Field of view 2.4 x 1.5 mm Culture vessels Flasks, well plates, petri dishes, slides Storage 50 GB cloud storage Operating conditions 5-40°C, 20-95% humidity Warranty 1 year parts & labor Image analysis Cloud-based
- 9. WWW.SCINTICA.COM Monitor your cells throughout their entire culture period 9
- 10. WWW.SCINTICA.COM Monitor your cells throughout their entire culture period 10
- 11. WWW.SCINTICA.COM Analysis Software 11 Confluency Algorithm Scratch / Wound Healing Algorithm
- 12. WWW.SCINTICA.COM Research Applications 12 • Image cell division • Stem cell differentiation • Monitor cell growth and confluency • Analyze cell migration and wound healing • Monitoring colony formation • Study chemotaxis
- 16. WWW.SCINTICA.COM Multi Lux • Combine up to 4 compact Lux2 devices • Automated live-cell imaging designed for long-term comparison studies and large laboratory teams • All devices connect to a single laptop optimizing lab space and remote access 16
- 19. WWW.SCINTICA.COM Lux3 FL: Technical Specifications 19 Specifications Lux3 FL System dimensions 166 x 140 x 135 mm Weight 1.3 kg Channels Brightfield, green and red fluorescence Magnification 10x fixed objective, digital zoom to 20x Fluorescence filters Excitation: 425/45 (green), 561/14 (red), Emission: Emission: 512/23 (green), 630/90 (red) Camera 6.4MP CMOS Image size 2072 x 2072 pixels Field of view 1.45 x 1.45 mm Culture vessels Flasks, well plates, petri dishes, slides Storage Unlimited cloud storage Operating conditions 5-40°C, 20-95% humidity Warranty 1 year parts & labor Image analysis Cloud-based
- 20. WWW.SCINTICA.COM Lux3 FL: Technical Specifications 20 Specifications Lux3 FL System dimensions 166 x 140 x 135 mm Weight 1.3 kg Channels Brightfield, green and red fluorescence Magnification 10x fixed objective, digital zoom to 20x Fluorescence filters Excitation: 425/45 (green), 561/14 (red), Emission: Emission: 512/23 (green), 630/90 (red) Camera 6.4MP CMOS Image size 2072 x 2072 pixels Field of view 1.45 x 1.45 mm Culture vessels Flasks, well plates, petri dishes, slides Storage Unlimited cloud storage Operating conditions 5-40°C, 20-95% humidity Warranty 1 year parts & labor Image analysis Cloud-based
- 21. WWW.SCINTICA.COM Why add fluorescence? 21 Brightfield Brightfield + Fluorescence
- 22. WWW.SCINTICA.COM Why add fluorescence? 22 Fluorescent /brightfield area
- 23. WWW.SCINTICA.COM Research Applications 23 • Viability • Transfection • Co-culture • Cellular processes • Nanoparticles
- 24. WWW.SCINTICA.COM OMNI: Technical Specifications 24 Specifications Omni System dimensions 396 x 345 x 171 mm Weight 9 kg Optics Brightfield with digital contrast Magnification 10x fixed objective – 517 pixels/mm Light source LED Camera 5MP CMOS Image size 99 x 131 pixels Output options Raw images, processed images and data Culture vessels Flasks, well plates, petri dishes, slides Storage 1-,5-, or 10 years of cloud storage Operating conditions 5-40°C, 20-95% humidity Warranty 1 year parts & labor Image analysis Cloud-based
- 25. WWW.SCINTICA.COM OMNI: Technical Specifications 25 Specifications Omni System dimensions 396 x 345 x 171 mm Weight 9 kg Optics Brightfield with digital contrast Magnification 10x fixed objective – 517 pixels/mm Light source LED Camera 5MP CMOS Image size 99 x 131 pixels Output options Raw images, processed images and data Culture vessels Flasks, well plates, petri dishes, slides Storage 1-,5-, or 10 years of cloud storage Operating conditions 5-40°C, 20-95% humidity Warranty 1 year parts & labor Image analysis Cloud-based
- 26. WWW.SCINTICA.COM Why the OMNI over the Lux2? 26
- 30. WWW.SCINTICA.COM Comparing the options… 30 Specifications Lux2 Lux3 FL OMNI System dimensions 133 x 90 x 100 mm 166 x 140 x 135 mm 396 x 345 x 171 mm Weight 0.5 kg 1.3kg 9 kg Optics Brightfield with digital contrast Brightfield, green and red fluorescence Brightfield with digital contrast Magnification 10x fixed objective, digital zoom to 20x 10x fixed objective, digital zoom to 20x 10x fixed objective – 517 pixels/mm Light source LED LED LED Fluorescence filters N/A Excitation: 425/45 (green), 561/14 (red) Emission: 512/23 (green), 630/90 (red) N/A Camera 5MP CMOS 6.4MP CMOS 5MP CMOS Image size 1280 x 720 pixels 2072 x 2072 pixels Variable FOV 2.4 x 1.5 mm 1.45 x 1.45 mm 91 x 131 mm Culture vessels Flasks, well plates, petri dishes, slides Flasks, well plates, petri dishes, slides Flasks, well plates, petri dishes, slides Storage Unlimited cloud storage Unlimited cloud storage 1-,5-, or 10 years of cloud storage Operating conditions 5-40°C, 20-95% humidity 5-40°C, 20-95% humidity 5-40°C, 20-95% humidity Warranty 1 year parts & labor 1 year parts & labor 1 year parts & labor Image analysis Cloud-based Cloud-based Cloud-based
- 31. WWW.SCINTICA.COM Get started immediately… 31 • Can use all channels separately and/or combined • Change the focus for each channel individually for all channels using Lock Z • Set the light intensity, exposure time and gain for each channel
- 34. WWW.SCINTICA.COM Summary 34 • Automated image capture: Don’t waste valuable time. Let the CytoSMART products capture fluorescent and brightfield images for you. • Time-lapse movies: Get the whole story. Observe the development of cellular events while they occur. • Red & green fluorescence: Broaden your horizon. Expand the number of variables you can examine using fluorescent labelling. • Integrated image analysis: Immediate insight in fluorescent area or fluorescent object number. • Incubator friendly: Small size fits all. Fluorescence live-cell imaging alongside cell culturing in the same incubator. • Plug-and-play: Get started instantly. Just a short training will get you up-and-running. • Cloud-based system: Inspect your cells anywhere, anytime. No more unnecessary lab visits. • Cost-effective system: Microscope with brightfield, green & red fluorescence, laptop and unlimited storage.
- 35. Audience Poll
- 36. WWW.SCINTICA.COM Scintica Instrumentation Phone: +1 (519) 857-6199 kparkins@scintica.com Q&A SESSION: To ask a question, click the Q&A Button, type your question and click send. Any questions that are not addressed during the live webinar will be answered following the event. Thank you for participating! Katie Parkins, PhD Applications Scientist