Labs mas
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The document discusses capacity building efforts at AfricaRice including short term training programs for scientists from various countries in West Africa and the creation of molecular biology labs in 4 countries. It also discusses long term training through PhD programs and the screening and evaluation of Near Isogenic Lines derived from parental lines containing resistance to Rice Yellow Mottle Virus. Traits such as resistance, yield, and plant characteristics were evaluated in multiple countries through collaboration with National Agricultural Research Systems.
Labs mas
- 1. Capacity Building Short term training • Two weeks intensive training at AfricaRice Dr Drissa Hema Mr Guindo Brema • Short term trainees: (Burkina Faso) (Mali) – 41 NARS scientists from Burkina Faso, Guinea, Gambia and Mali – 1 technician – 1 Research Assistant Dr Soungalo Sara (Mali) – 2 PhD students Mr Mon Seraphin (Guinea) • Practical exercise on data analyses Long term training: and interpretation 6 PhD students – Marker assisted selection
- 2. Capacity Building 4 molecular biology labs created in 4 countries Training of a total of 29 NARS scientists (breeders), lab technicians, researc h assistants and Guinea students from Burkina Burkina-Faso Faso, Guinea, Mali, The Gambia The Gambia Mali
- 3. Field screening of Near isogenic Lines (NILs) derived from FRK28 in a RYMV hotspot at Edhozigi (Nigeria) FKR28 parental lines FKR28-NIL 16 + rymv1-2 gene
- 4. Field screening of Near isogenic Lines (NILs) derived from IR64 in a RYMV hotspot at Edhozigi (Nigeria) IR64-NIL 130 IR64 parental lines + rymv1-2 gene
- 5. MAS: NILs lab and in country evaluations NILs derived from the RYMV MAS project and 7 parents Seeds distributed to NARS for in country evaluation 42 lines are virus free Foreground of lines is being check to further confirm the presence of rymv1-2 Background of donors parents genome is being check - Symptoms scored from 1 to 9 at 14, 21, 28, and 42 DAI - Plant Height and SPAD at 28 and 42 DAI - Virus content determined by ELISA - 4 resistant lines were tested in 7 NARS for resistance, yield and related traits for release (Project CORAF-AfricaRice) - 33 others sisters lines are available for selection