PRESENTED BY
MANJUSHREE MASHAL
2GI14LDN07
1
2
 INTRODUCTION
 HISTORY
 MATERIALS AND METHODS
 FIELD IMAGE SPECTROSCOPY
 RESULTS AND DISCUSSION
 IMAGING SPECTROSCOPY APPLICATIONS
 CONCLUSION AND FUTURE WORK
 REFERENCE
NIR spectroscopy is a vibrational spectroscopic method belongs to
the infrared light spectrum which is very close to the visible region
(from about 750 to 2500 nm).
3
Figure 1.1 Range of Electromagnetic Radiation
NIR is a spectroscopic method based on molecular overtones and combination
of C-H ,O-H,N-H ions.
There are two laws which govern the basics of vibrational spectroscopy
 Hooke’s law and
 Frank principle
4
Figure 1.2: Vibrational transition
Chlorophyll Estimation using NIR
 Chlorophyll is an extremely important biomolecule, critical in
photosynthesis, which allows plants to absorb energy from light.
The chlorophyll content in leaf is an indicator of grow health
condition and the soybean yield.
 Imaging spectroscopy has great potential for estimating chlorophyll
content of soybean dynamic, rapidly.
 The objective of this presentation is to combine random forests and
field imaging spectroscopy for estimating chlorophyll content in
soybean at leaf scale.
5
Three centuries ago Sir Isaac Newton published in his ‘Treatise of Light’
the concept of dispersion of light.
 The history of NIR is begins with William Herschel in 18th century.
 The term spectroscopy was first used in the late 19th century and provided the
empirical foundations for atomic and molecular physics.
 A large number of spectral indices have been developed for the study of
chlorophyll content based on leaf reflectance. Reviews of different spectral
indices developed for estimating chlorophyll content are offered by Bannari et
al. (2007), He et al. (2006) and Haboudane et al. (2008).
6
7
8
A. Study site
Field spectroscopy was carried out with an ASD FieldSpec3 in summer 2009,
at the farmlands of city of Chang’chun, Jinlin province. Chlorophyll content
of soybean was taken as researching target.
The spectral range between 350-1250 nm was used for the retrieval of leaf
chlorophyll concentration. Leaf chlorophyll concentration in soybean was
measured by SPAD502.
SPAD 502 Plus Chlorophyll Meter
9
B. Field campaign data
Field campaigns in the soybean field were carried out under clear sky
conditions. Reflectance of soybean was collected by ASD3 spectrometer,
with a 350-2500nm spectral range.
C. PROSPECT model
PROSEPCT is a radiative transfer model to simulate the leaf reflectance
spectra with the range of 400 to 2500 nm. And it only needs a small amount
of input parameters like chlorophyll content, moisture, dry matter content
and structure parameter.
 Random forests is a classification and regression
algorithm originally designed for statistical machine
learning method, which is created by Breiman.
 Random forests were adopted to train the training
data set, in order to establish leaf chlorophyll content
estimation model.
 Advantages: Non parametric,
Non sensitive to overfitting,
Calculate classification error.
10
11
FIELD IMAGE SPECTROSCOPY
A field imaging spectrometer system was designed for agriculture applications.
FISS was used to gather spectral information from soybean leaves. Leaf
estimation model of chlorophyll concentration was applied to the validation data
set to estimate leaf chlorophyll content of soybean in the research area and
validation data set was established based on proximal hyperspectral data.
FISS CONSISTS OF
 Opto-mechanical system: Scanning mirror, Optical lenses,
Spectroscopic devices and a Charge-coupled device (CCD)
camera.
 Electronic system: Power and Motor control circuits
 computer system: hardware and software
Hardware is a portable laptop computer, and the software
includes the FISS operating software, data acquisition
software and data processing software.
12
13
Actual photograph of FISS field measurements.
 Linear regression (LR)
 Stepwise multiple linear regression (SMLR)
 Partial least squares regression (PLSR)
 Support-vector machine (SVM) regression
14
15
STATISTICAL DESCRIPTION OF SOYBEAN AT
SAMPLING SITES
16
Effect of number of trees in RF-PROSPECT model
for estimating chlorophyll content.
17
Validation of the soybean chlorophyll content
estimation model.
• Fast
• Simple sample preparation
• Non destructive
• Online for process applications
• Need for calibration
• Opportunity for data analysis
Overlapping bands (combination), not easy to interpret.
Usually not for trace level analysis.
20
APPLICATIONS
Forecasting corn yield with imaging spectroscopy.
Image spectroscopy to mapping canopy concentration in the
nitrogen.
Near Infrared Spectroscopic Analysis in the Food Industry and
Research.
Image spectroscopy in Forest application.
Other Application are
 Imaging spectroscopy for scene analysis.
 Mineral mapping and Application.
 Medical field
 Industrial uses
 Astronomical spectroscopy
21
CONCLUSION
22
This work established the soybean chlorophyll based on random forests and
PROSPECT, The estimation model can be used as an effective tool for estimation
of soybean chlorophyll content, and can be adopted to precision agriculture
management.
Future study will concentrated on scaled up the field estimation model to
satellite remote sensing level, which will monitor the soybean’s health condition in
a large scale.
2. D.Sathis Kumar et al“ Near Infra Red Spectroscopy- An Overview” International
Journal of ChemTech Research CODEN( USA): IJCRGG ISSN : 0974-4290 Vol. 3,
No.2, pp 825-836, April-June 2011.
3.M.E. Schaepman, S. L. Ustin, and A. J. Plaza, “Earth system science related
imaging spectroscopy—An assessment, ”. Remote Sensing of Environment, vol.
113, pp. 123-S137, 2009.
4.J. Delegido,L. Alonso, and L.G. Gonzalez, “Estimating chlorophyll content of crops
from hyperspectral data using a normalized area over reflectance curve (NAOC)”.
International Journal of Applied Earth Observation and Geoinformation, vol. 13,
pp. 165-174, 2010.
5. P.O. Gislason, J.A. Benediktsson, and J.R. Sveinsson, “Random Forests for land
cover classification”, Pattern Recognition Letters, vol. 27, pp. 294-300, 2006.
23
1. Jie Lv, Zhenguo Yan “Estimating Leaf Chlorophyll Concentration in Soybean
Using Random Forests and Field Imaging Spectroscopy”IEEE Transactions On
Geoscience And Remote Sensing.
24
THANK YOU
25

More Related Content

PPTX
RECENT DIGNOSTIC TECHNIQUES AND AMELIORATIVE MEASURES OF NUTRIENTS DEFICIENCI...
PPTX
B Sc Agri II Sc,Sf & Nm, U 1 Soil And Plant Nutrient
PPTX
Functions and deficiency of Iron, boron and zinc
PPTX
kiwifruit production in india
PPTX
Various media for propagating nursery plants
PPTX
Scarification and stratification of seeds
PPTX
contact fungicide
PPT
Red Cotton Bug
RECENT DIGNOSTIC TECHNIQUES AND AMELIORATIVE MEASURES OF NUTRIENTS DEFICIENCI...
B Sc Agri II Sc,Sf & Nm, U 1 Soil And Plant Nutrient
Functions and deficiency of Iron, boron and zinc
kiwifruit production in india
Various media for propagating nursery plants
Scarification and stratification of seeds
contact fungicide
Red Cotton Bug

What's hot (20)

PPTX
Inflorescence, Flower, Flower structures, Mode of Pollination, Seed fromation...
PPTX
Exercise-4 Preservation.pptx
PPT
Nutrient recycling through agricultural and industrial wastes:potential and l...
PPTX
Soil fertility evaluation
PPTX
B.sc. agri i po h unit 4.7 cultivation practices of mango
PDF
Meterological instruments
PPTX
Introduction, importance and Scope of horticulture.pptx
PPTX
Production technology of ginger
PPT
Fruit set, growth & developmental stages of various fruits
PDF
Determination of total phosphorus in plant materials
DOCX
Production Technology Of Sugarcane
PPTX
Nutrient use efficiency
PPTX
Marigold
PPT
Grafting in vegetable
PDF
Unit 1 lecture-1 soil fertility and soil productivity
PPTX
Ssac 353 lecture no. 13 and 14 n fertilizers classification, fate of n fert...
PPTX
PPTX
Soil fertility evaluation and fertilizer recommendation
PPTX
Grey Blight in Horticultural Crops
PPTX
Pigeonpea
Inflorescence, Flower, Flower structures, Mode of Pollination, Seed fromation...
Exercise-4 Preservation.pptx
Nutrient recycling through agricultural and industrial wastes:potential and l...
Soil fertility evaluation
B.sc. agri i po h unit 4.7 cultivation practices of mango
Meterological instruments
Introduction, importance and Scope of horticulture.pptx
Production technology of ginger
Fruit set, growth & developmental stages of various fruits
Determination of total phosphorus in plant materials
Production Technology Of Sugarcane
Nutrient use efficiency
Marigold
Grafting in vegetable
Unit 1 lecture-1 soil fertility and soil productivity
Ssac 353 lecture no. 13 and 14 n fertilizers classification, fate of n fert...
Soil fertility evaluation and fertilizer recommendation
Grey Blight in Horticultural Crops
Pigeonpea
Ad

Viewers also liked (16)

PDF
13_CV_Johannes Gerard
PPTX
2017 9-17教學連繫
PDF
unlocking the full potential of women in Czech business
DOCX
La descolonización a nivel mundial
PPTX
Semana 7 formulacion
PPTX
Nsm goa 2016
PPTX
Planificación familiar
PPTX
metodos anticonceptivos
PDF
PROJECT REPORT ON Introduction of SIGNA Cabins in Prima Trim Line 1
PDF
Variables operacionalización
PPTX
Imageprocessing
PPTX
Metodos Anticonceptivos - Barrera, hormonales, conductales y otros.
PPT
Introduction to Greenhouse Diseases Seminar for ProGreen2009
PPTX
PPTX
Media and Information Literacy (MIL) - Digital Citizenship, Netiquette, Digit...
PDF
Role of Talent Management
13_CV_Johannes Gerard
2017 9-17教學連繫
unlocking the full potential of women in Czech business
La descolonización a nivel mundial
Semana 7 formulacion
Nsm goa 2016
Planificación familiar
metodos anticonceptivos
PROJECT REPORT ON Introduction of SIGNA Cabins in Prima Trim Line 1
Variables operacionalización
Imageprocessing
Metodos Anticonceptivos - Barrera, hormonales, conductales y otros.
Introduction to Greenhouse Diseases Seminar for ProGreen2009
Media and Information Literacy (MIL) - Digital Citizenship, Netiquette, Digit...
Role of Talent Management
Ad

Similar to Leaf chlorophyll concentration using random forest (20)

PDF
NIR in Leaf Chlorophyll Concentration Estimation
PPT
Application of spectral remote sensing in agronomic decision by Dr.V.Harihara...
PDF
Nir meat milk
PDF
study and analysis of hy si data in 400 to 500
PDF
Anomaly Detection in Fruits using Hyper Spectral Images
PDF
Laser Ablation Molecular Isotopic Spectrometry for rare isotopes of the light...
PDF
A Infrared hyperspectral imaging technique for non-invasive cancer detection.
PDF
A new technique for infrared remote sensing of solar induced fluorescence and...
PDF
Formatted Invited SPIE paper (2)
PDF
Investigation of Chaotic-Type Features in Hyperspectral Satellite Data
PPTX
Applications of infrared spectroscopy
PDF
Hyperspectral Remote Sensing Of Vegetation Using Red Edge Position Techniques
PDF
Spatial Laser Induced Fluorescence Imaging (SLIFIMG) Analysis to Assess Tissu...
PPT
Application Of Nirs In Feed Industry
PPT
Application Of NIRS In Feed Industry
PPTX
Modern analytical techniques used to characterize organic compounds. Birbhum ...
PDF
Mutli vendor mrsi_2020
PDF
Development and Validation of a Combined Photoacoustic Micro-Ultrasound Syste...
PDF
Diferrentes experimentos a traves de la espectrocopia aplicada en biotecnologias
PDF
Non Invasive Glucose Measurement using Raman Spectroscopy
NIR in Leaf Chlorophyll Concentration Estimation
Application of spectral remote sensing in agronomic decision by Dr.V.Harihara...
Nir meat milk
study and analysis of hy si data in 400 to 500
Anomaly Detection in Fruits using Hyper Spectral Images
Laser Ablation Molecular Isotopic Spectrometry for rare isotopes of the light...
A Infrared hyperspectral imaging technique for non-invasive cancer detection.
A new technique for infrared remote sensing of solar induced fluorescence and...
Formatted Invited SPIE paper (2)
Investigation of Chaotic-Type Features in Hyperspectral Satellite Data
Applications of infrared spectroscopy
Hyperspectral Remote Sensing Of Vegetation Using Red Edge Position Techniques
Spatial Laser Induced Fluorescence Imaging (SLIFIMG) Analysis to Assess Tissu...
Application Of Nirs In Feed Industry
Application Of NIRS In Feed Industry
Modern analytical techniques used to characterize organic compounds. Birbhum ...
Mutli vendor mrsi_2020
Development and Validation of a Combined Photoacoustic Micro-Ultrasound Syste...
Diferrentes experimentos a traves de la espectrocopia aplicada en biotecnologias
Non Invasive Glucose Measurement using Raman Spectroscopy

More from Manjushree Mashal (16)

PPTX
Career in cyber security
PPTX
Cyber security
PPTX
Cyber security
PPTX
Cyber attack
PPTX
Dos attack
PPTX
Sql injection
PPTX
Xss attack
PPTX
Network packet analysis -capture and Analysis
PPTX
Network attacks
PPTX
Network forensic
ODP
TCP/IP FRAME FORMAT
PPTX
Diabetic Retinopathy Analysis using Fundus Image
DOCX
Manjushree_EC_fresher_2016
PPTX
Tvws ppt 1
PPT
Vlsi design and fabrication ppt
PPTX
underwater communication skills for the new way of devine(2)
Career in cyber security
Cyber security
Cyber security
Cyber attack
Dos attack
Sql injection
Xss attack
Network packet analysis -capture and Analysis
Network attacks
Network forensic
TCP/IP FRAME FORMAT
Diabetic Retinopathy Analysis using Fundus Image
Manjushree_EC_fresher_2016
Tvws ppt 1
Vlsi design and fabrication ppt
underwater communication skills for the new way of devine(2)

Recently uploaded (20)

PDF
Abrasive, erosive and cavitation wear.pdf
PPTX
Amdahl’s law is explained in the above power point presentations
PDF
distributed database system" (DDBS) is often used to refer to both the distri...
PPTX
CN_Unite_1 AI&DS ENGGERING SPPU PUNE UNIVERSITY
PPTX
CyberSecurity Mobile and Wireless Devices
PPTX
Feature types and data preprocessing steps
PDF
Applications of Equal_Area_Criterion.pdf
PDF
20250617 - IR - Global Guide for HR - 51 pages.pdf
PPTX
ai_satellite_crop_management_20250815030350.pptx
PDF
Implantable Drug Delivery System_NDDS_BPHARMACY__SEM VII_PCI .pdf
PDF
Java Basics-Introduction and program control
PPTX
mechattonicsand iotwith sensor and actuator
PPTX
Measurement Uncertainty and Measurement System analysis
PPTX
Principal presentation for NAAC (1).pptx
PDF
UEFA_Carbon_Footprint_Calculator_Methology_2.0.pdf
PDF
Design Guidelines and solutions for Plastics parts
PPT
Chapter 1 - Introduction to Manufacturing Technology_2.ppt
PDF
August 2025 - Top 10 Read Articles in Network Security & Its Applications
PDF
LOW POWER CLASS AB SI POWER AMPLIFIER FOR WIRELESS MEDICAL SENSOR NETWORK
PDF
Computer System Architecture 3rd Edition-M Morris Mano.pdf
Abrasive, erosive and cavitation wear.pdf
Amdahl’s law is explained in the above power point presentations
distributed database system" (DDBS) is often used to refer to both the distri...
CN_Unite_1 AI&DS ENGGERING SPPU PUNE UNIVERSITY
CyberSecurity Mobile and Wireless Devices
Feature types and data preprocessing steps
Applications of Equal_Area_Criterion.pdf
20250617 - IR - Global Guide for HR - 51 pages.pdf
ai_satellite_crop_management_20250815030350.pptx
Implantable Drug Delivery System_NDDS_BPHARMACY__SEM VII_PCI .pdf
Java Basics-Introduction and program control
mechattonicsand iotwith sensor and actuator
Measurement Uncertainty and Measurement System analysis
Principal presentation for NAAC (1).pptx
UEFA_Carbon_Footprint_Calculator_Methology_2.0.pdf
Design Guidelines and solutions for Plastics parts
Chapter 1 - Introduction to Manufacturing Technology_2.ppt
August 2025 - Top 10 Read Articles in Network Security & Its Applications
LOW POWER CLASS AB SI POWER AMPLIFIER FOR WIRELESS MEDICAL SENSOR NETWORK
Computer System Architecture 3rd Edition-M Morris Mano.pdf

Leaf chlorophyll concentration using random forest

  • 2. 2  INTRODUCTION  HISTORY  MATERIALS AND METHODS  FIELD IMAGE SPECTROSCOPY  RESULTS AND DISCUSSION  IMAGING SPECTROSCOPY APPLICATIONS  CONCLUSION AND FUTURE WORK  REFERENCE
  • 3. NIR spectroscopy is a vibrational spectroscopic method belongs to the infrared light spectrum which is very close to the visible region (from about 750 to 2500 nm). 3 Figure 1.1 Range of Electromagnetic Radiation
  • 4. NIR is a spectroscopic method based on molecular overtones and combination of C-H ,O-H,N-H ions. There are two laws which govern the basics of vibrational spectroscopy  Hooke’s law and  Frank principle 4 Figure 1.2: Vibrational transition
  • 5. Chlorophyll Estimation using NIR  Chlorophyll is an extremely important biomolecule, critical in photosynthesis, which allows plants to absorb energy from light. The chlorophyll content in leaf is an indicator of grow health condition and the soybean yield.  Imaging spectroscopy has great potential for estimating chlorophyll content of soybean dynamic, rapidly.  The objective of this presentation is to combine random forests and field imaging spectroscopy for estimating chlorophyll content in soybean at leaf scale. 5
  • 6. Three centuries ago Sir Isaac Newton published in his ‘Treatise of Light’ the concept of dispersion of light.  The history of NIR is begins with William Herschel in 18th century.  The term spectroscopy was first used in the late 19th century and provided the empirical foundations for atomic and molecular physics.  A large number of spectral indices have been developed for the study of chlorophyll content based on leaf reflectance. Reviews of different spectral indices developed for estimating chlorophyll content are offered by Bannari et al. (2007), He et al. (2006) and Haboudane et al. (2008). 6
  • 7. 7
  • 8. 8 A. Study site Field spectroscopy was carried out with an ASD FieldSpec3 in summer 2009, at the farmlands of city of Chang’chun, Jinlin province. Chlorophyll content of soybean was taken as researching target. The spectral range between 350-1250 nm was used for the retrieval of leaf chlorophyll concentration. Leaf chlorophyll concentration in soybean was measured by SPAD502. SPAD 502 Plus Chlorophyll Meter
  • 9. 9 B. Field campaign data Field campaigns in the soybean field were carried out under clear sky conditions. Reflectance of soybean was collected by ASD3 spectrometer, with a 350-2500nm spectral range. C. PROSPECT model PROSEPCT is a radiative transfer model to simulate the leaf reflectance spectra with the range of 400 to 2500 nm. And it only needs a small amount of input parameters like chlorophyll content, moisture, dry matter content and structure parameter.
  • 10.  Random forests is a classification and regression algorithm originally designed for statistical machine learning method, which is created by Breiman.  Random forests were adopted to train the training data set, in order to establish leaf chlorophyll content estimation model.  Advantages: Non parametric, Non sensitive to overfitting, Calculate classification error. 10
  • 11. 11 FIELD IMAGE SPECTROSCOPY A field imaging spectrometer system was designed for agriculture applications. FISS was used to gather spectral information from soybean leaves. Leaf estimation model of chlorophyll concentration was applied to the validation data set to estimate leaf chlorophyll content of soybean in the research area and validation data set was established based on proximal hyperspectral data.
  • 12. FISS CONSISTS OF  Opto-mechanical system: Scanning mirror, Optical lenses, Spectroscopic devices and a Charge-coupled device (CCD) camera.  Electronic system: Power and Motor control circuits  computer system: hardware and software Hardware is a portable laptop computer, and the software includes the FISS operating software, data acquisition software and data processing software. 12
  • 13. 13 Actual photograph of FISS field measurements.
  • 14.  Linear regression (LR)  Stepwise multiple linear regression (SMLR)  Partial least squares regression (PLSR)  Support-vector machine (SVM) regression 14
  • 15. 15 STATISTICAL DESCRIPTION OF SOYBEAN AT SAMPLING SITES
  • 16. 16 Effect of number of trees in RF-PROSPECT model for estimating chlorophyll content.
  • 17. 17 Validation of the soybean chlorophyll content estimation model.
  • 18. • Fast • Simple sample preparation • Non destructive • Online for process applications • Need for calibration • Opportunity for data analysis
  • 19. Overlapping bands (combination), not easy to interpret. Usually not for trace level analysis.
  • 20. 20 APPLICATIONS Forecasting corn yield with imaging spectroscopy. Image spectroscopy to mapping canopy concentration in the nitrogen. Near Infrared Spectroscopic Analysis in the Food Industry and Research. Image spectroscopy in Forest application.
  • 21. Other Application are  Imaging spectroscopy for scene analysis.  Mineral mapping and Application.  Medical field  Industrial uses  Astronomical spectroscopy 21
  • 22. CONCLUSION 22 This work established the soybean chlorophyll based on random forests and PROSPECT, The estimation model can be used as an effective tool for estimation of soybean chlorophyll content, and can be adopted to precision agriculture management. Future study will concentrated on scaled up the field estimation model to satellite remote sensing level, which will monitor the soybean’s health condition in a large scale.
  • 23. 2. D.Sathis Kumar et al“ Near Infra Red Spectroscopy- An Overview” International Journal of ChemTech Research CODEN( USA): IJCRGG ISSN : 0974-4290 Vol. 3, No.2, pp 825-836, April-June 2011. 3.M.E. Schaepman, S. L. Ustin, and A. J. Plaza, “Earth system science related imaging spectroscopy—An assessment, ”. Remote Sensing of Environment, vol. 113, pp. 123-S137, 2009. 4.J. Delegido,L. Alonso, and L.G. Gonzalez, “Estimating chlorophyll content of crops from hyperspectral data using a normalized area over reflectance curve (NAOC)”. International Journal of Applied Earth Observation and Geoinformation, vol. 13, pp. 165-174, 2010. 5. P.O. Gislason, J.A. Benediktsson, and J.R. Sveinsson, “Random Forests for land cover classification”, Pattern Recognition Letters, vol. 27, pp. 294-300, 2006. 23 1. Jie Lv, Zhenguo Yan “Estimating Leaf Chlorophyll Concentration in Soybean Using Random Forests and Field Imaging Spectroscopy”IEEE Transactions On Geoscience And Remote Sensing.
  • 24. 24