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LIST OF CELL BASED ASSAY

 S. No  Assay Code    Name of the Assay                           Stimulant                            Model
IMMUNOMODULATION
   1   CA-IM001                                                    Naive
   2   CA-IM001A                                                   Con A
   3   CA-IM001B   Lymphocyte Proliferation                         LPS
                                                                                       Murine Macrophage/Human PBMC
   4   CA-IM001C   Assay                                            PHA
   5   CA-IM001D                                                   PWA
   6   CA-IM001E                                                  Anti-CD3
   7   CA-IM002    Macrophage Phagocytosis                           ---               Murine Macrophages
   8   CA-IM003    Mediator Release (IL-2)                         Con A
   9   CA-IM004    Mediator Release (IL-2)                          PHA
                                                                                       Murine Splenocytes/ Human PBMC
  10   CA-IM005    Mediator Release (IL-2)                        Anti-CD3
  11   CA-IM006    Mediator Release (IL-2)                         PWA
  12   CA-IM007    Mediator Release (IL-3)                           Con A             Murine Splenocytes/ Human PBMC
  13   CA-IM008      Mediator Release (IL-4)                         Con A             Murine Splenocytes/ Human PBMC
  14   CA-IM009      Mediator Release (IL-6)                       Con A
  15   CA-IM010      Mediator Release (IL-6)                        LPS
  16   CA-IM011      Mediator Release (IL-6)                        PHA                Murine Splenocytes/ Human PBMC
  17   CA-IM012      Mediator Release (IL-6)                       PWA
  18   CA-IM013      Mediator Release (IL-6)                      Anti-CD3
  19   CA-IM014      Mediator Release (IL-10)                      Con A
                                                                                       Murine Splenocytes/ Human PBMC
  20   CA-IM015      Mediator Release (IL-10)                       LPS

                    R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India.

                      Tel: 91-080-40209996-9, Fax: 91-080-40209817, E-mail: cvc@naturalremedy.com
LIST OF CELL BASED ASSAY

 S. No Assay Code     Name of the Assay                           Stimulant                            Model
IMMUNOMODULATION
  21   CA-IM016   Mediator Release (IL-12)                            LPS              Murine Splenocytes/ Human PBMC
  22   CA-IM017   Mediator Release (IFN-γ)                           Con A             Murine Splenocytes/ Human PBMC
  23   CA-IM018   Nitric oxide Release                                 ---             Murine macrophages
ANTI-INFLAMMATION
  24   CA-AI001   Nitric oxide scavenging                             LPS              Murine Macrophages
  25   CA-AI002   Mediator Release (IL-1β)                            LPS              Murine Macrophages
  26   CA-AI003   Mediator Release (IL-6)                             LPS              Murine Macrophages
  27   CA-AI004   Mediator Release (TNF-α)                            LPS              Murine Macrophages
  28   CA-AI005   Mediator Release (PGE2)                             LPS              Murine Macrophages
                                                                                       Human promyelocytic leukemia
 29    CA-AI006    Mediator Release (LTB4)                          A23187
                                                                                       cells
 30   CA-AI007     Mediator Release (TXB2)                          A23187             Murine Macrophages
 31   CA-AI008     Mediator Release (MCP-1)                          LPS               Murine Macrophages
ANTI-ALLERGY
 32   CA-AA001     Mediator Release (IL-5)                           Con A             Murine Splenocytes/ Human PBMC
                   Mediator Release
 33    CA-AA002                                                     A23187             Rat Basophilic leukemia cells
                   (Histamine)
COSMETOLOGY
 34  CA-CO001      Collagen Release                                   ---              Human Dermal Fibroblast
 35  CA-CO002      Melanin Release                                  α-MSH              Human Melanocytes



                    R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India.

                      Tel: 91-080-40209996-9, Fax: 91-080-40209817, E-mail: cvc@naturalremedy.com
LIST OF CELL BASED ASSAY

S. No Assay Code    Name of the Assay                              Stimulant                           Model
DIABETES AND OBESITY
  36  CA-DO001 Insulin Release                                           ---            Mouse beta cell insulinoma cell
  37  CA-DO002 Adipogenesis                                              ---            Mouse embryonic fibroblast
  38  CA-DO003 Adipolysis                                                ---            Mouse embryonic fibroblast
LIVER HEALTH
  39  CA-LH001 Hepatoprotection                                     t-BH
  40  CA-LH002 Hepatoprotection (OPC)                             Diazinon
                                                                              Human hepatocellular liver
  41  CA-LH003 Hepatoprotection (OC)                                Aldrin
                                                                              carcinoma cell
  42  CA-LH004 Hepatoprotection                                 Aflatoxin B1
  43  CA-LH005 Hepatoprotection                                 Galactosamine
ONCOLOGY
                                                                                        Human hepatocellular liver
 44    CA-ON001    Cellular antioxidant assay                            ---
                                                                                        carcinoma cell
  45   CA-ON002 Anti-Proliferation                                       ---            Human breast adenocarcinoma cell
IN-VITRO GENOTOXICITY
                Chromosomal aberration
  46   CA-IG001                                                          ---            Chinese Hamster Ovary
                assay
                                                                                        cell/Human PBMC
  47   CA-IG002 Micronucleus test                                        ---
                Bacterial Reverse Mutation
  48   CA-IG003                                                          ---            Salmonella typhimurium, E. Coli
                Assay
OTHERS
  49   CA-OA001 Cytotoxicity Assay                                       ---            Mouse embryonic fibroblast


                    R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India.

                      Tel: 91-080-40209996-9, Fax: 91-080-40209817, E-mail: cvc@naturalremedy.com

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List of cell based assay

  • 1. LIST OF CELL BASED ASSAY S. No Assay Code Name of the Assay Stimulant Model IMMUNOMODULATION 1 CA-IM001 Naive 2 CA-IM001A Con A 3 CA-IM001B Lymphocyte Proliferation LPS Murine Macrophage/Human PBMC 4 CA-IM001C Assay PHA 5 CA-IM001D PWA 6 CA-IM001E Anti-CD3 7 CA-IM002 Macrophage Phagocytosis --- Murine Macrophages 8 CA-IM003 Mediator Release (IL-2) Con A 9 CA-IM004 Mediator Release (IL-2) PHA Murine Splenocytes/ Human PBMC 10 CA-IM005 Mediator Release (IL-2) Anti-CD3 11 CA-IM006 Mediator Release (IL-2) PWA 12 CA-IM007 Mediator Release (IL-3) Con A Murine Splenocytes/ Human PBMC 13 CA-IM008 Mediator Release (IL-4) Con A Murine Splenocytes/ Human PBMC 14 CA-IM009 Mediator Release (IL-6) Con A 15 CA-IM010 Mediator Release (IL-6) LPS 16 CA-IM011 Mediator Release (IL-6) PHA Murine Splenocytes/ Human PBMC 17 CA-IM012 Mediator Release (IL-6) PWA 18 CA-IM013 Mediator Release (IL-6) Anti-CD3 19 CA-IM014 Mediator Release (IL-10) Con A Murine Splenocytes/ Human PBMC 20 CA-IM015 Mediator Release (IL-10) LPS R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India. Tel: 91-080-40209996-9, Fax: 91-080-40209817, E-mail: cvc@naturalremedy.com
  • 2. LIST OF CELL BASED ASSAY S. No Assay Code Name of the Assay Stimulant Model IMMUNOMODULATION 21 CA-IM016 Mediator Release (IL-12) LPS Murine Splenocytes/ Human PBMC 22 CA-IM017 Mediator Release (IFN-γ) Con A Murine Splenocytes/ Human PBMC 23 CA-IM018 Nitric oxide Release --- Murine macrophages ANTI-INFLAMMATION 24 CA-AI001 Nitric oxide scavenging LPS Murine Macrophages 25 CA-AI002 Mediator Release (IL-1β) LPS Murine Macrophages 26 CA-AI003 Mediator Release (IL-6) LPS Murine Macrophages 27 CA-AI004 Mediator Release (TNF-α) LPS Murine Macrophages 28 CA-AI005 Mediator Release (PGE2) LPS Murine Macrophages Human promyelocytic leukemia 29 CA-AI006 Mediator Release (LTB4) A23187 cells 30 CA-AI007 Mediator Release (TXB2) A23187 Murine Macrophages 31 CA-AI008 Mediator Release (MCP-1) LPS Murine Macrophages ANTI-ALLERGY 32 CA-AA001 Mediator Release (IL-5) Con A Murine Splenocytes/ Human PBMC Mediator Release 33 CA-AA002 A23187 Rat Basophilic leukemia cells (Histamine) COSMETOLOGY 34 CA-CO001 Collagen Release --- Human Dermal Fibroblast 35 CA-CO002 Melanin Release α-MSH Human Melanocytes R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India. Tel: 91-080-40209996-9, Fax: 91-080-40209817, E-mail: cvc@naturalremedy.com
  • 3. LIST OF CELL BASED ASSAY S. No Assay Code Name of the Assay Stimulant Model DIABETES AND OBESITY 36 CA-DO001 Insulin Release --- Mouse beta cell insulinoma cell 37 CA-DO002 Adipogenesis --- Mouse embryonic fibroblast 38 CA-DO003 Adipolysis --- Mouse embryonic fibroblast LIVER HEALTH 39 CA-LH001 Hepatoprotection t-BH 40 CA-LH002 Hepatoprotection (OPC) Diazinon Human hepatocellular liver 41 CA-LH003 Hepatoprotection (OC) Aldrin carcinoma cell 42 CA-LH004 Hepatoprotection Aflatoxin B1 43 CA-LH005 Hepatoprotection Galactosamine ONCOLOGY Human hepatocellular liver 44 CA-ON001 Cellular antioxidant assay --- carcinoma cell 45 CA-ON002 Anti-Proliferation --- Human breast adenocarcinoma cell IN-VITRO GENOTOXICITY Chromosomal aberration 46 CA-IG001 --- Chinese Hamster Ovary assay cell/Human PBMC 47 CA-IG002 Micronucleus test --- Bacterial Reverse Mutation 48 CA-IG003 --- Salmonella typhimurium, E. Coli Assay OTHERS 49 CA-OA001 Cytotoxicity Assay --- Mouse embryonic fibroblast R & D Centre: Plot # 5B, 19th K.M Stone, Hosur Road, Bangalore – 560 100, India. Tel: 91-080-40209996-9, Fax: 91-080-40209817, E-mail: cvc@naturalremedy.com